Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

大洋在大西洋气候变化中的作用

冰期剧烈的气候变化与大西洋环流的变化有关。在过去 1 0ｋａ间 ,气候较为稳定 ,百年级波动相对温和。目前 ,全球年复一年的气候变化主要由太平洋厄尔尼诺—南方涛动所引起。太平洋的赤道宽度是大西洋的 3倍 ,但不致于影响全球气候 ;大西洋的影响范围虽小 ,然而却非常明显 ,尤其是在环流改变时 ,国际研究项目 (气候变化与可预见性 )将对大西洋气候变化机制和可预见性做深入的探讨。热带大西洋地区大气 海洋相互作用 ,在春季 (3— 5月 )引起巴西至加勒比海的南美东海岸、在秋季 (8— 9月 )引起非洲亚撒哈拉西部年际降雨量和时间的明显变化。…     

中美洲古地理制约了上新世北冰洋软体动物的迁移

白令海峡首次张开和中美洲航道关闭是新生代晚期两个最重要的古地理和海洋事件。除了其区域生物地理效应外 ,这些事件改变了北太平洋和北大西洋航道的形态 ,对北半球大洋环流和气候产生了重要影响。白令海峡首次张开所产生的最引人注目的结果是软体动物在北太平洋、北冰洋和北大西洋之间的交流。这一事件被称为“横越北冰洋的交流” ,涉及２９５个软体动物种 ,其中源于太平洋的２６１个 ,源于大西洋—北冰洋的３４个。由于新第三纪晚期的这种交流 ,现今北冰洋软体动物的大部分源于北大西洋 ,尤其表现在北美北冰洋和西伯利亚东北部海岸 ,而大…     

An Experi mental Study on Wave-Induced Dynamic Stresses in Seabed

1 .IntroductionEvery year ,Taiwanis subjectedtotwo or three typhoonintrusions in average which always causedisasters . Most of the disasters which occur around the coastal zone are due to seabed instability andcoastal structure destruction caused bytyphoo…     

等离子体技术及其在地质上的应用

激光烧蚀微探针等离子体质谱的地质应用。自1989年以来，我们实验室就积极开展了激光烧蚀微探针(LAM)试样引入系统的研究工作，这种技术适合于ICP—MS分析地质样品中小体积矿物(激光烧蚀孔穴直径低于10—100μm)．对某些样品．采用Nd：YAG激光的基本IR波长(1064nm)，作为多数用途，4倍频率(uv)的波长(266nm)能产生较小的直径，并较好地形成孔穴．     

赤道太平洋的环流

一.引 言 赤道太平洋上层海洋环流的主要特征已被建立十多年了。1942年斯维尔德鲁普等人描述了中太平洋的表面流:往西流的北和南赤道流以及往东流的北赤道逆流。随后,南赤道逆流也被用表面动力高度图表示出来(Reia,1961),Cromwell等人(1954)发现了赤道潜流,从而对上述环流的描述进行了主要修正。Hisard和Rual(1970)发现了赤道潜流下面     

Evaluating the effects of protection on fish predators and sea urchins in shallow artificial rocky habitats: a case study in the northern Adriatic Sea

Man-made defence structures (e.g., breakwaters, jetties) are becoming common features of marine coastal landscapes all around the world. The ecology of assemblages of species associated with such artificial structures is, however, poorly known. In this study, we evaluated the density and size of fish predators of echinoids (i.e., Diplodus sargus, Diplodus vulgaris, Sparus aurata), and the density of sea urchins (i.e., Paracentrotus lividus) at defence structures (i.e., breakwaters) inside and outside the marine protected area of Miramare (northern Adriatic Sea) in order to: (1) assess possible differences in fish predator density and size between protected and fished breakwaters; (2) assess whether fish predation may have the potential to affect sea urchin density in artificial rocky habitats. Surveys were carried out at four random times over a period of two years. Total density, and density of medium- and large-sized individuals of the three predatory fishes were generally greater at the protected than at the fished breakwaters, whereas no differences were detected in the density of small-sized individuals. Density of the sea urchin P. lividus did not show any difference between protected and fished breakwaters. The results of this study suggest that: (1) protection may significantly affect predatory fishes in artificial rocky habitats; (2) differences in predatory fish density, and size may be unrelated with the density of the sea urchin P. lividus; (3) protected artificial structures such as breakwaters, originally planned for other purposes, could represent a potential tool for fish population recovery and enhancement of local fisheries.     

旧照片显示澳大利亚珊瑚礁的破坏

旧照片显示澳大利亚珊瑚礁的破坏ＩａｎＡｎｄｅｒｓｏｎ澳大利亚大堡礁的许多部分正在以令人吃惊的速度消失。昆士兰州Ｔｏｗｎｓｖｉｌｌｅ的大堡礁海洋公园管理局的科学家把现代大堡礁的照片与早到１８９０年拍摄的照片进行了比较，为其发现深感震惊。“某种东西正在毁...     

贝加尔裂谷系及邻区断裂现今活动变化及成因(英文)

已有的地质和地球物理方法无法对年、月、旬等现今时间尺度上的断层活动变化进行有效预测。运用基于地震活动定量指数运算程序的地理信息技术可以解决这个问题。这种方法被应用于贝加尔裂谷系(BRS)及邻区的研究当中。研究发现,断层活动变化发生在以几年为周期的时间尺度上,这无法用区域应力场的变化进行合理解释。沿着穿越贝加尔裂谷系的剖面编制了活动断层图以及地震活动定量指数曲线。提出的这种方法可以根据地震活动定量指数对活动断层进行仔细的分类,从而为解决地震中期预报相关问题开辟了重要途径。这种方法还被用来研究断层现今活动的时空变化和形成机制。研究发现,在实时尺度上断层活动具有高频发生的特征,这种现象可能是由于脆性岩石圈板内和块体内部运动产生的慢变形波的干扰导致的。利用变形波的通过速度可以对活动断层进行分组,各组具有不同的地质和地球物理特征参数,同时还可以对变形波波前的方向以及某一区域内主要断层在实时间隔内(地质上的瞬间)的活动情况进行预测。     

The population of Croatia

Croatia is a Central European and Mediterranean state, located in contact with and under the influence of various spheres of civilization, which for centuries have penetrated and conflicted on and around the territory of Croatia. Such a position has resulted in various influences which did not always have a positive effect on the development of Croatia. The gradual narrowing and decrease of the Croatian ethnic territory, as well as the presence of national minorities in it, was also a result of the aforementioned position and outside influences (ulji 1993/1994).The demographic structure of Croatia indicates a series of specificities which were primarily conditioned by the historical development of Croatia and which is particularly expressed in constant emigration since the end of the nineteenth century, the relatively large direct and indirect losses to the population during and immediately after the First and Second World Wars, emigration as a type of population movement in all inter-census periods after 1945, the appearance of a natural decline and the aging of the population on almost one half of the state territory.