Differential developmental toxicity of naphthoic acid isomers in medaka (Oryzias latipes) embryos

Polycyclic aromatic hydrocarbons (PAHs) are widespread persistent pollutants that readily undergo biotic and abiotic conversion to numerous transformation products in rivers, lakes and estuarine sediments. Here we characterize the developmental toxicity of four PAH transformation products each structural isomers of hydroxynaphthoic acid: 1H2NA, 2H1NA, 2H3NA, and 6H2NA. Medaka fish (Oryzias latipes) embryos and eleutheroembryos were used to determine toxicity. A 96-well micro-plate format was used to establish a robust, statistically significant platform for assessment of early life stages. Individual naphthoic acid isomers demonstrated a rank order of toxicity with 1H2NA>2H1NA>2H3NA>6H2NA being more toxic. Abnormalities of circulatory system were most pronounced including pericardial edema and tube heart. To determine if HNA isomers were AhR ligands, spatial-temporal expression and activity of CYP1A was measured via in vivo EROD assessments. qPCR measurement of CYP1A induction proved different between isomers dosed at respective concentrations affecting 50% of exposed individuals (EC50s). In vitro, all ANH isomers transactivated mouse AhR using a medaka CYP1A promoter specific reporter assay. Circulatory abnormalities followed P450 induction and response was consistent with PAH toxicity. A 96-well micro-plates proved suitable as exposure chambers and provided statistically sound evaluations as well as efficient toxicity screens. Our results demonstrate the use of medaka embryos for toxicity analysis thereby achieving REACH objectives for the reduction of adult animal testing in toxicity evaluations.     

A Comparison Between GEBCO Sheet 5.17 and the International Bathymetric Chart of the Arctic Ocean (IBCAO) Version 1.0

In 1979, the General Bathymetric Chart of the Oceans (GEBCO) published Sheet 5.17 in the Fifth Edition of its series of global bathymetric maps. Sheet 5.17 covered the northern polar region above 64° N, and was for long the authoritative portrayal of Arctic bathymetry. The GEBCO compilation team had access to an extremely sparse sounding database from the central Arctic Ocean, due to the difficulty of mapping in this permanently ice covered region. In the past decade, there has been a substantial increase in the database of central Arctic Ocean bathymetry, due to the declassification of sounding data collected by US and British Navy nuclear submarines, and to the capability of modern icebreakers to measure ocean depths in heavy ice conditions. From these data sets, evidence has mounted to indicate that many of the smaller (and some larger) bathymetric features of Sheet 5.17 were poorly or wrongly defined. Within the framework of the project to construct the International Bathymetric Chart of the Arctic Ocean (IBCAO), all available historic and modern data sets were compiled to create a digital bathymetric model. In this paper, we compare both generally and in detail the contents of GEBCO Sheet 5.17 and version 1.0 of IBCAO, two bathymetric portrayals that were created more than 20 years apart. The results should be helpful in the analysis and assessment of previously published studies that were based on GEBCO Sheet 5.17. Ron Macnab: Retired.     

Constituency election campaigning under the alternative vote: the New South Wales Legislative Assembly election 1995

Summary Most studies of the impact of local campaigning on the geography of election results in single-member constituency electoral systems have been conducted in countries where the 'first-past-the-post' system is employed. Australia uses the 'alternative vote' (AV) system for elections to the federal and some state Lower Houses, with parties competing for both first- and lower-preference votes. This paper analyses the relationship between spending and success in the contests for both types of votes at the 1995 New South Wales State Legislative Assembly election, with significant findings: the more intense a party's campaign, ceteris paribus, the more first-preference votes it obtained and the more lower-preference votess when these were reallocated. The AV system was recommended for adoption in the UK by a government-commissioned report in 1998, and the paper draws some implications for the UK situation.     

局部桩筏基础的设计与施工

以加拿大多伦多市某工程为例,介绍了在复杂地质条件下采用局部桩筏基础(PPRF)的设计及施工问题;探讨了设计PPRF的决定性因素;在保持PPRF设计的完整性前提下,提出了单位沉降量的准则,并用于筏板和桩的设计;计算了PPRF的滑移及转动;最后,采用有效方法对该工程采用的局部桩筏基础进行了计算分析.结果表明,PPRF的设计主要取决于侧向土压力、分布不均的建筑荷载以及地基土的非均匀承载力,工程桩应主要布置在沉降较大的区域,即位于筏板基础承受高压力而土体承载力较低的西北部.探讨局部桩筏基础的设计与施工为该类型工程的基础设计提供了一个新的解决途径.     

Decade time-scale modulation of low-mass X-ray binaries

Regular observations by the All-Sky Monitor aboard the Rossi X-ray Timing Explorer satellite have yielded well-sampled light curves with a time baseline of over 10 years. We find that up to eight of the 16 brightest persistent low-mass X-ray binaries (LMXBs) show significant, possible sinusoidal, variations with periods of the order of 10 years. We speculate on its possible origin and prevalence in the population of LMXBs, and we find the presence of a third object in the system, or long-period variability intrinsic to the donor star, as being attractive origins for the X-ray flux modulation we detect. For some of the objects in which we do not detect a signal, there is substantial short-term variation which may hide modest modulation on long time-scales. Decade time-scale modulations may thus be even more common.     

Geomagnetic field intensity: How high can it get? How fast can it change? Constraints from Iron Age copper slag

The intensity of the geomagnetic field varies over different time scales. Yet, constraints on the maximum intensity of the field as well as for its maximum rate of change are inadequate due to poor temporal resolution and large uncertainties in the geomagnetic record. The purpose of this study is to place firm limits on these fundamental properties by constructing a high-resolution archaeointensity record of the Levant from the 11th century to the early 9th century BCE, a period over which the geomagnetic field reached its maximum intensity in Eurasia over the past 50,000 years. We investigate a ¹⁴C-dated sequence of ten layers of slag material, which accumulated within an ancient industrial waste mound of an Iron Age copper-smelting site in southern Israel. Depositional stratigraphy constrains relative ages of samples analyzed for paleointensity, and ¹⁴C dates from different horizons of the mound constrain the age of the whole sequence. The analysis yielded 35 paleointenisty data points with accuracy better than 94% and precision better than 6%, covering a period of less than 350 years, most probably 200 years. We construct a new high-resolution quasi-continuous archaeointensity curve of the Levant that displays two dramatic spikes in geomagnetic intensity, each corresponding to virtual axial dipole moment (VADM) in excess of 200 ZAm². The geomagnetic spikes rise and fall over a period of less than 30 years and are associated with VADM fluctuations of at least 70 ZAm². Thus, the Levantine archaeomagnetic record places new constraints on maximum geomagnetic intensity as well as for its rate of change. Yet, it is not clear whether the geomagnetic spikes are local non-dipolar features or a geomagnetic dipolar phenomenon.     

Micro-relief development in polygonal patterned ground in the Dry Valleys of Antarctica

Polygonal patterned ground in polar regions of both Earth and Mars has received considerable attention. In comparison with the size, shape, and arrangement of the polygons, the diverse micro-relief and topography (termed here simply “relief”) of polygonal patterned ground have been understudied. And yet, the relief reflects important conditions and processes occurring directly below the ground surface, and it can be observed readily in the field and through remote sensing. Herein, we describe the relief characteristic of the simplest and relatively young form of patterned ground in the Dry Valleys of Antarctic. We also develop a numerical model to examine the generation of relief due to subsurface material being shouldered aside contraction cracks by incremental sand wedges growth, and to down-slope creep of loose granular material on the surface. We model the longterm subsurface deformation of ice-cemented permafrost as a non-linear viscous material. Our modeling is guided and validated using decades of field measurements of surface displacements of the permafrost and relief. This work has implications for assessing the activity of surfaces on Earth and Mars, and much larger scale potential manifestations of incremental wedging in icy material, namely the distinct paired ridges on Europa.     

Atmospheric circulation anomalies during two persistent north american droughts: 1932�C1939 and 1948�C1957

We use an early twentieth century (1908?C1958) atmospheric reanalysis, based on assimilation of surface and sea level pressure observations, to contrast atmospheric circulation during two periods of persistent drought in North America: 1932?C1939 (the ??Dust Bowl??) and 1948?C1957. Primary forcing for both droughts is believed to come from anomalous sea surface temperatures (SSTs): a warm Atlantic and a cool eastern tropical Pacific. For boreal winter (October?CMarch) in the 1950s, a stationary wave pattern originating from the tropical Pacific is present, with positive centers over the north Pacific and north Atlantic ocean basins and a negative center positioned over northwest North America and the tropical/subtropical Pacific. This wave train is largely absent for the 1930s drought; boreal winter height anomalies are organized much more zonally, with positive heights extending across northern North America. For boreal summer (April?CSeptember) during the 1930s, a strong upper level ridge is centered over the Great Plains; this feature is absent during the 1950s and appears to be linked to a weakening of the Great Plains low-level jet (GPLLJ). Subsidence anomalies are co-located over the centers of each drought: in the central Great Plains for the 1930s and in a band extending from the southwest to the southeastern United States for the 1950s. The location and intensity of this subsidence during the 1948?C1957 drought is a typical response to a cold eastern tropical Pacific, but for 1932?C1939 deviates in terms of the expected intensity, location, and spatial extent. Overall, circulation anomalies during the 1950s drought appear consistent with the expected response to the observed SST forcing. This is not the case for the 1930s, implying some other causal factor may be needed to explain the Dust Bowl drought anomalies. In addition to SST forcing, the 1930s were also characterized by massive alterations to the land surface, including regional-scale devegetation from crop failures and intensive wind erosion and dust storms. Incorporation of these land surface factors into a general circulation model greatly improves the simulation of precipitation and subsidence anomalies during this drought, relative to simulations with SST forcing alone. Even with additional forcing from the land surface, however, the model still has difficulty reproducing some of the other circulation anomalies, including weakening of the GPLLJ and strengthening of the upper level ridge during AMJJAS. This may be due to either weaknesses in the model or uncertainties in the boundary condition estimates. Still, analysis of the circulation anomalies supports the conclusion of an earlier paper (Cook et?al. in Proc Natl Acad Sci 106:4997, 2009), demonstrating that land degradation factors are consistent with the anomalous nature of the Dust Bowl drought.