Effects of pulp bleach plant effluents on hepatic xenobiotic biotransformation enzymes in fish: Laboratory and field studies

The present preliminary report on studies of the influence of pulp bleach plant effluents on hepatic biotransformation enzymes in fish is a part of a major characterisation of biochemical and physiological effects of effluents from pulp industries in Sweden. Our studies on the xenobiotic biotransformation enzymes demonstrate, so far, that the pulp effluents have the potential to strongly induce 7-ethoxyresorufin-O-deethylase activity (EROD) in fish liver and that fish living in the receiving water of an effluent from a pulp bleach plant have induced hepatic EROD activities as well as higher hepatic UDP glucuronyltransferase activities than those living in an unpolluted area. It is thus apparent that measures of induction of biotransformation enzymes in fish liver may be a useful approach in monitoring the presence of potential hazardous compounds released from pulp industries.     

Comparison of cytochrome P450 in three butterflyfish species: Ecological implications of elevated CYP2B and CYP3A in Chaetodon capistratus

The relationship between cytochrome P450 and feeding on terpenoid-rich gorgonian corals was investigated in a species of tropical butterflyfish and compared with two other sympatric congeners that do not feed on gorgonians. Fish were collected from non-polluted waters in Belize and the levels of two cytochrome P450 isozymes (CYP2B and CYP3A) were immunoquantitated in addition to quantification of total P450. Chaetodon capistratus regularly feeds on gorgonian corals and has higher levels of total hepatic microsomal cytochrome P450 than C. ocellatus or C. striatus. The content of hepatic P450 (0.588–0.794 nmol mg⁻¹) in C. capistratus is among the highest ever reported in teleosts from non-polluted waters and is significantly greater than detected in C. ocellatus or C. striatus. Chaetodon capistratus also had a larger hepatic index (g liver per g fish) and more microsomal protein (mg protein per g liver), factors that translate into 3.3- to 8-fold more total P450 per g fish. Sexual differences in total P450 were observed between male and female C. capistratus, but not among the other species. The contents of proteins detected by immunoassay with polyclonal anti-scup P450B (CYP2B) and anti-human P4503A (CYP3A) were 2- to 10-fold and 2- to 20-fold greater, respectively, in C. capistratus than in the congeneric species. CYP2 and CYP3 gene families in mammals are thought to have evolved partially in response to dietary allelochemicals. These results suggest that these P450 isozymes may also be important in marine teleosts that feed on terpenoid-rich prey.     

The effect of effluent high in chromium on marine sediment aerobic heterotrophic bacteria

A survey of the aerobic heterotrophic bacteria present in sediments at Sawyers Bay, New Zealand, receiving tannery effluent high in Cr, and a control site, indicates that the populations present are different and show seasonal variation. The bacterial population present at the polluted site appears more able to tolerate CrIII at concentrations less than 0·2 μmol ml^?1.     

Dynamics of ship collisions

This paper described a procedure for simulation of the outer dynamics in ship collisions.The simulation procedure is derived using the transient equations for the horizontal motion of a ship. The hydrodynamic forces acting on the ships' hull during the collision are calculated by a strip method, where the forces acting on each section are described by means of unit response functions. These functions are determined by cosine transformation of the sectional dampings. The sectional added masses and dampings, and thereby also the sectional unit response functions, are calculated by an approximate method. The deformations of the slip structures during the collisions are modelled as non-linear springs.The resulting system of non-linear equations is solved using a numerical time-integration procedure.A number of different collision situations are simulated by means of the procedure.