The Japanese medaka (Oryzias latipes) model: applicability for investigating the immunosuppressive effects of the aquatic pollutant benzo[a]pyrene (BaP)

Despite the fact that BaP is a carcinogen, mammalian immunosuppressant, and ubiquitous aquatic pollutant, knowledge regarding the effects of BaP on the immune system of fish is still lacking. To begin to fill this gap, studies were conducted in medaka to examine the effects and mechanisms by which BaP exposure might alter host immunocompetence. Fish, exposed by IP injection of BaP (2-600 microg/g BW), were examined after 48 h for effects upon immune function and CYP1A expression/activity. Benzo[a]pyrene, at a concentration below that which increased levels of CYPIA expression/activity (2 microg BaP/g BW) suppressed lymphocyte proliferation. Concentrations of BaP at 20 and 200 microg/g BW. suppressed antibody-forming cell (AFC) numbers, superoxide production, and host resistance against bacteria. In contrast, exposure to the low affinity aryl hydrocarbon receptor (AhR) agonist, benzo[e]pyrene (BeP), neither induced CYP1A expression nor altered immune function. Given the lack of immunosuppressive effects produced by BeP, and the fact that exposure to the AhR antagonist (and CYP1A inhibitor) alpha-naphthoflavone (ANF) ameliorated the suppressive effects of BaP upon AFC numbers, the AhR pathway (including CYP1A-mediated production of reactive BaP metabolites) appears important in mediating BaP-induced immunotoxicity in fish, as in mammals. In the past, the medaka has proven a successful model for assessing carcinogenic agents. These studies have demonstrated its utility for also determining the immunosuppressive effects of an important aquatic contaminant.     

Effect of shallow and narrow water on added mass of cylinders with various cross-sectional shapes

The sway, heave and roll added masses of three uniform cylinders with semi-circular, rectangular and triangular cross-sectional shapes in shallow and narrow water are numerically analysed. The method is based on simulation of the potential flow induced by the cylinder's mode of motion. The effects of shallow and narrow water on added mass are analysed and presented. It is concluded that the shallow and narrow water effects on added mass depend on the different cross-section shapes of the cylinders. In particular, the water depth effect on sway added mass is stronger than that on heave added mass while the narrow water effect on sway is weaker than that on heave. The shallow water effect on added mass tends to weaken the narrow water effect. Lastly the effect of shallow and narrow water on added mass on a rectangular cylinder is the strongest while that on a triangular cylinder is the weakest.     

On the radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas

The radiation and diffraction of linear water waves by an infinitely long rectangular structure submerged in oblique seas of finite depth is investigated. The analytical expressions for the radiated and diffracted potentials are derived as infinite series by use of the method of separation of variables. The unknown coefficients in the series are determined by the eigenfunction expansion matching method. The expressions for wave forces, hydrodynamic coefficients and reflection and transmission coefficients are given and verified by the boundary element method. Using the present analytical solution, the hydrodynamic influences of the angle of incidence, the submergence, the width and the thickness of the structure on the wave forces, hydrodynamic coefficients, and reflection and transmission coefficients are discussed in detail.     

中国近海的蔓足类 Ⅰ.藤壶属

藤壶属(Genus Balanus)是甲壳类蔓足亚纲(Subclass Cirripedia)藤壶科(Family Balanidae)中最大的一属，它在有害海洋附着生物中是主要组成成分。许多种附着于船底、水下设施或建筑物上，致使船舰燃料消耗增加，航速减低，降低浮标浮力，缩小管道通路，侵占某些水产养殖对象的有效附着面，污损养殖架筏和绳索，加快水下金属的腐蚀等，对海防、海运交通、工业和渔业常造成一定危害，同人类的关系颇为密切。随着我国科学事业的发展，特别是附着生物防除工作的进展，对于种类的确切鉴定和生物学及生态学资料需要日益增多，只有在搞清种类的基础上，根据不同种的生物学和生态特征，采取相应的措施，才能有效地进行防除工作。 中国近海的藤壶属，国内外仅有一些零星记载，缺乏全面而系统的报告。我们早在五十年代，就注意到我国沿海常见的一些主要附着生物，特别是当时作为“布纹藤壶” Balanus Amphitrite communis Darwin(=B.reticulatus Utinomi)和“克氏藤壶”Balanus Amphitrite krügeri Nilsson-Cantell(=B.uliginosus Utinomi)描记的两个类型，无论在栖息环境、繁殖习性或幼虫形态上都有显著差异，因而怀疑它们不是一个种内的亚种，而应属于不同的种。为了解决上述和类似的问题，给生态和防除研究工作提供更为准确的基本资料，我们对藤壶亚目的种类进行了系统的研究，本文是研究报告的第一部分。根据的是中国科学院海洋研究所历年采集收藏的标本，共鉴定31种和亚种，分隶于7个亚属，其中有6新种，有9种在我国是首次记录(种名录见190页)。新种的模式标本均保存于中国科学院海洋研究所。     

A low level supplementary heating system for free divers

A simple and effective method of heating divers in extreme cold water has been developed. The local heating concept utilizes a uniformly distributed granular mixture of magnesium and iron particles packed in small sachets. Upon activation by sea water, the two metal mixture behaves as a multitude of short-circuited electrolytic cells, producing thermal energy rather than electrical energy. The 45 mm-square sachets may be placed where heating is required on the diver's body, thus giving rise to the term “local heating”, obviously, the heating system requires no pumping device, or distribution network. Heating rate and duration of output of the sachets are controlled by particle size, and mixture ratio of the constituent Mg and Fe particles.This paper described the development, testing and performance of the heating sachets. Results of live tests in different dive situations are also presented and discussed.     

Seasonal variation of Mn adsorption on to calcareous surfaces in the English Channel, and its implication on the manganese distribution coefficient

Dissolved and particulate manganese in seawater samples derived from the English Channel has been analyzed using graphite furnace atomic absorption (GFAAS), and inductively coupled plasma atomic emission (ICP—AES) spectroscopies. Because of the high contents of carbonate minerals found in the suspended matter samples, the English Channel constitutes an ideal field area for the study of the Mn²⁺/Ca²⁺/CaCO₃ system. Owing to the chemical speciation of particulate manganese and the combined use of the X-ray diffraction and electron spin resonance spectroscopy we have shown the importance of the carbonate phase in the stabilization of manganese (II). This has been confirmed by p-pH measurements in the field. All these studies have also indicated that: (1) manganese is associated with calcite in the form of a solid solution, Mn_xCa_1−xCO₃; and (2) significant increases in the concentrations of particulate manganese, especially in offshore waters, occur in summer. This seasonal phenomenon has been attributed to the proliferation of coccolithophorids, which are known to be covered with calcified skeletons at high specific surface areas. To appraise the implication of the coccolithophorid-blooms phenomenon on the Mn²⁺/Ca²⁺/CaCO₃ system, we have used the manganese distribution coefficient, D_i, between the liquid phase and CaCO₃ particles. Overall we have shown that: (1) D_i in summer (i.e. when coccoliths considered as very fine-grained calcite are abundant) is much higher than that obtained in winter; and (2) in the vicinity of the French coast, D_i does not vary significantly even in summer. This is because of the high content of chalk-derived particles found in the near-shore waters.     

The intertidal erosion rate of cohesive sediment: a case study from Long Island Sound

Over the past decades, many attempts have been made to generate useful bottom erosion models for the study of cohesive sediment movement. This study addresses some of the key questions involved in determining the functional relationship between erosion rate and bottom shear stress. Current, wave, and turbidity data were collected from a bottom mounted instrument array in a moderately energetic estuarine environment. The bottom shear stress was calculated from a wave–current interaction model. The erosion rate was derived from the observed sediment concentration using a vertical mixing model. Examination of the relationship between erosion rate and bottom stress showed that the erosion rate varied at intertidal frequency. When averaged over the tidal fluctuation, the erosion rate remained approximately constant at low stress, but increased sharply when the shear stress rose above a critical value. This suggests two-stage erosion. The bed has a layered structure, in which a thin layer of loose, high water content material overlies a more consolidated bed. The top layer of high water content material (fluff) was easily disturbed and re-suspended by tidal currents, but the consolidated bottom layer was eroded only under conditions of high shear stress.     

Post-Miocene stratigraphy and depositional environments of valley-fill sequences at the mouth of Tampa Bay, Florida

Post-Miocene sea-level low stands allowed rivers and karst processes to incise the exposed carbonate platform along the Gulf Coast of Florida. Few Miocene to mid-Pleistocene deposits survived erosion along the present coast except within incised valleys. Since their formation, these valleys have been filled and incised multiple times in response to sea-level changes. The thick sedimentary sequences underlying the mouth of Tampa Bay have been recorded as a range of depositional environments and multiple sea-level incursions and excursions during pre-Holocene time and subsequent to the accumulation of the Miocene carbonate sequences. Sediment analysis of cores collected from a north–south transect across the mouth of Tampa Bay has enabled the identification of lithofacies, ranging from well-sorted, quartz sand to dense, fossiliferous, phosphatic grainstone. These facies were deposited in freshwater, estuarine, and shallow, open marine environments. As a result of channel development and migration within the paleovalley, and cut-and-fill associated with individual transgressions and regressions, correlation of the lithofacies does not extend across the entire transect. Fining-upward sequences truncated by tidal ravinement surfaces that extend throughout the paleovalley can, however, be identified. Age determinations based on 14-C analysis, amino-acid racemization, and strontium isotope analysis dating of numerous samples yield ages of Miocene, Pliocene, early Pleistocene, and late Pleistocene, as well as Holocene for sequences that accumulated and were preserved in this valley-fill complex. Numerous inconsistencies in the stratigraphic organization of the age determinations indicate that there are bad dates, considerable reworking of shells that were dated, or both. For this reason as well as the lack of detailed correlation among the three relatively complete cores, it is not possible to place these strata in a sequence stratigraphic framework.