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51.
Characterization of bacterial community structure in vestimentiferan tubeworm Ridgeia piscesae trophosomes 总被引:1,自引:0,他引:1
Terminal‐restriction fragment length polymorphisms (T‐RFLPs) were used to address the question whether there are multiple phylotypes of bacteria within the trophosome of the vestimentiferan tubeworm Ridgeia piscesae in addition to the known endosymbiont. Clone libraries were constructed to aid in the identification of the additional bacterial phylotypes. Individual R. piscesae specimens were collected from Juan de Fuca Ridge, Axial Caldera and Explorer Ridge, Magic Mountain. Clone library analyses revealed only one bacterial phylotype (expected R. piscesae endosymbiont) within the Explorer Ridge trophosomes. However, the Axial Caldera clone library revealed five operational taxonomic units (OTUs). Three of the resulting phylotypes detected (designated RAE) were putative thioautotrophic symbionts within the γ‐Proteobacteria which belonged to the anticipated endosymbiont group (RAE OTU 1), the Maorithyas hadalis symbiont II group (RAE OTU 3), and the Halothiobacillus group (RAE OTU 4). The remaining two phylotypes were most likely opportunistically derived. RAE OTU 5 was an α‐Proteobacterium within the Roseobacter group and RAE OTU 2 was within the Cytophaga–Flavobacterium–Bacteroidetes. T‐RFLP analyses revealed that all 15 trophosomes evaluated contained at least RAE OTUs 1 and 4. Five of the 15 trophosomes contained at least RAE OTUs 1, 3, and 4 and two of the trophosomes contained at least five RAE OTUs. Cluster analysis of the T‐RFLP data revealed three distinct clusters. The number of taxa within the trophosome may be an indication of the general health of the tubeworm. This study strongly suggests that we have discovered and identified more than one bacterial phylotype within the trophosome of vestimentiferan R. piscesae. 相似文献
52.
53.
The hepatic mixed function oxidase system in the fish differs from that in mammals in its responses to the two classic mammalian inducers. The cytochrome P-448-type inducers (polycyclic aromatic hydrocarbons) stimulate monooxygenase activity, but phenobarbital, a P-450-type inducer, does not.1 We have compared the effects of phenobarbital (PB) and polychlorinated biphenyls (PCB) on the turnover of hepatic microsomal hemoproteins in trout (PCB's are P-448- and P-450-type inducers in mammals, which in fish induce only cytochrome P-448). We show here that neither PCB nor PB treatment changed the turnover rate. However, both the rates of synthesis and degradation were much slower than in the rat. 相似文献
54.
John Hardy Steven Kiesser Liam Antrim Alan Stubin Richard Kocan John Strand 《Marine environmental research》1987,23(4)
The sea surface is an important habitat for the developmental stages (eggs and larvae) of many fish and invertebrates; it is also a concentration point for anthropogenic contaminants entering the sea. Studies were conducted to determine the extent to which the sea surface of Puget Sound was toxic to the early life history stages of fish. Three urban bays with suspected contamination, a rural reference bay, and a Central Sound site were compared. Surface-dwelling eggs and organisms (zooneuston) were collected with a surface-skimming neuston net and their densities enumerated. Sand sole (Psettichthys melanostictus) embryos were exposed in the field and laboratory to the sea-surface microlayer. To develop a useful year-round approach to monitoring sea-surface toxicity, larval development of anchovies, kelp bass, and sea urchins was also evaluated as an indication of sea-surface microlayer toxicity.During the spawning season (February and March), urban boys in Puget Sound had lower concentrations of sand sole eggs and neustonic organisms on the sea surface than did the rural bayor Central Sound reference sites. Compared to the reference sites, laboratory exposure to surface microlayer samples collected from urban bay sites generally resulted in more chromsomal aberrations in developing sole embryos, reduced hatching success of sole larvae, and reduced growth in trout cell cultures. In situ hatching success of sole eggs was reduced by half or more in urban bays compared to reference sites.Toxicity was associated with visible surface slicks and, in urban bays, increased with increasing surface pressure (dynes cm−1). Results to be reported separately (Part II) indicate that toxicity is strongly correlated with the presence of high concentrations of polycyclic aromatic hydrocarbons and metals in the sea-surface microlayer. The toxicity of SMIC samples was similar when evaluated by sole, anchovy, kelp bass, or sea urchin tests. A sea-surface monitoring program could use sea urchin embryos to evaluate site-specific sea-surface toxicity throughout the year. 相似文献
55.
An experimental facility is described for the testing of structures under a combined axial load and hydrostatic pressure load. This facility was used to determine the effects of hydrostatic pressure (up to 6000 psi) on the load-carrying capacity of slender metal columns. There appeared to be no significant effect in this case. 相似文献
56.
An automatic sample changer based upon a commercially available 20-port rotary valve has been developed for use in automated seawater nutrient analyses. All parts exposed to seawater are of stainless steel, Teflon®, or Kel-F®. The sampler has proven to be rugged and efficient in both shore and shipboard laboratories. 相似文献
57.
Implementation of an expert system for the design of single-point, subsurface oceanographic moorings
The design of single-point, subsurface moorings is carried out a present by a design engineer with the help of some computer programs. These computer programs are used for analyzing the mooring, but the setting-up of the initial mooring configuration and the subsequent modifications on the basis of the results of the analysis are carried out by the design engineer.An expert system for mooring design is developed to eliminate the human expert from the design process and thus to enable a novice to design a mooring by himself. Using the expert system, an optimum least-weight in air design can be developed in much less time than is usually required in conventional procedures. Perhaps the most significant advantage is that the system can serve as a vehicle for effective transfer of present day expertise for future applications.The system is developed and implemented on an IBM PC/AT. It has a user-friendly, interactive, menu-driven input procedure and a sophisticated output facility. The design process is totally automated and the requirement to consult a human expert is easily eliminated. The system has tremendous flexibility and a knowledge engineer can easily adapt it to the specific requirements of a particular user. The concept of machine learning is introduced by recording the failure data and using this information in the later decision-making processes. A CAD package, AUTOCAD, is used to generate the drawings and an interface is developed between the CAD package and the expert system so that the former is transparent to the user. 相似文献
58.
Geological and geophysical surveys of active submarine volcanoes offshore and southeast of Epi Island, Vanuatu, New Hebrides
Arc, have delineated details of the structure and acoustic stratigraphy of three volcanic cones. These submarine cones, named
Epia, Epib, and Epic, are aligned east-west and spaced 3.5 km apart on the rim of a submerged caldera. At least three acoustic
sequences, of presumed Quaternary age, can be identified on single-channel seismic-reflection profiles. Rocks dredged from
these cones include basalt, dacite, and cognate gabbroic inclusions with magmatic affinities similar to those of the Karua
(an active submarine volcano off the southeastern tip of Epi) lavas. 相似文献
59.
The anthropogenic contribution of trace metals to settling particulate matter (SPM) and surficial sediments was determined on the high-energy continental shelf adjacent to Sydney, Australia. Settling particulate matter and surficial sediment was collected in the vicinity of a major sewage outfall and at five control sites on the middle shelf (80-100 m water depth). Sediment traps were deployed on 10 occasions for up to 2 weeks during the summer and winter of 1995 and SPM was analyzed for Ag, Co, Cr, Cu, Fe, Mn, Ni, Pb and Zn. Cobalt, Fe, Mn and Ni act conservatively in SPM and in sediments regionally and are used as normalizing elements to determine anthropogenic enrichment. Surficial sediments and SPM are enriched in Ag, Cr, Cu, Pb and Zn near a major ocean outfall and at four of the five control sites, although sewage particles contribute < 5% of trace metals in the total sample. Silver is the most sensitive trace metal tracer for establishing the presence of sewage particulate matter. Sewage particulate matter flux near the outfall was estimated using a two end-member mixing model and is below 0.5 g m(-2) day(-1) during all deployment periods. The mean sewage particulate matter flux at sampling locations 30 km and 60 km north of the outfall are <0.13 g m(-2) day(-1) and <0.01 g m(-2) day(-1), respectively, indicating an efficient dispersal of anthropogenic material on this high energy continental shelf. 相似文献
60.
Killifish (Fundulus heteroclitus) from a highly contaminated site on the Elizabeth River are resistant to the acute toxicity and the cytochrome P4501A (CYP1A)-inducing activity of both the sediments from the site and chemically pure polycyclic aromatic hydrocarbons (PAHs). These effects are highly heritable for one generation, but heritable to a lesser degree by subsequent generations, in clean conditions in the laboratory. We show that offspring of this population of Elizabeth River killifish are also resistant to the teratogenicity and P4501A-inducing activity of PCB congener 126, a prototypical coplanar halogenated aromatic hydrocarbon (HAH). Furthermore, the pattern of greater resistance to acute toxicity and P4501A-inducing activity in the first generation and less in subsequent generations is also observed upon exposure to PCB-126. 相似文献