Culture-dependent and independent analyses of subsurface microbial communities in oil-bearing strata of the Sagara oil reservoir

Abstract   Culture-dependent and independent methods were used to evaluate the microbial communities in cores collected at depths up to 200 m in oil-bearing and oil-free strata near the Sagara oil reservoir near Shizuoka, Japan. Direct microbial counts revealed much higher numbers (2.2 × 10⁴−7.9 × 10⁶/g) of microbes in the oil-bearing strata than in the oil-free zones, where counts were uniform at approximately 1.0 × 10⁴/g. Molecular taxonomic analyses via 16S rRNA gene sequence comparisons showed that the oil-free strata were dominated by members of the γ-Proteobacteria including Pseudomonas , Stenotrophomonas and Sphingomonas , whereas the oil-bearing strata were dominated by a single species closely related to Pseudomonas stutzeri . All archaeal clones were phylogenetically affiliated with the uncultured soil group in Crenarchaeota with the exception of a single phylotype that belonged to the genus Thermococcus . Culture-dependent analysis was carried out by most-probable-number culturing as well as direct plating to determine viable cell counts, using both complex organic substrates or native oil and autotrophic media. Both culture-dependent and independent methods revealed the abundant cultivable member was the aerobic oil-degrading bacterium Pseudomonas stutzeri and neither autotrophs nor anaerobic heterotrophs could be detected in the oil-bearing strata.     

Chemical reaction diversity of geofluids revealed by hydrothermal experiments under sub- and supercritical states

Abstract   Hydrothermal experiments of dissolution of granite and quartz with pure water up to 600°C and 60 MPa were carried out in order to evaluate chemical reaction under sub- and supercritical conditions. The supercritical region beyond the critical point for water has been inferred to be a homogeneous state, which does not correspond to either a true liquid phase, or a true vapor phase. Results of dissolution experiments of granite and quartz in a supercritical state (artificially defined as higher pressures and temperatures than the critical point), show this fluid can be subdivided into two apparent phases comprising a 'liquid-like' region and a 'vapor-like' region. Chemical phenomena with respect to dissolution reactions in the 'liquid-like' region are more similar to those of subcritical water, whilst those reactions in the 'vapor-like' region even in the supercritical state are considered to be weak. The critical point of various kinds of geofluids, composed of solutions in the H₂O-CO₂-NaCl system was experimentally determined using a visible type autoclave. Chemical reaction diversity, depending on location of the critical point for a given solution and apparent phase boundary in terms of chemical reaction within the supercritical state, was recognized by hydrothermal experiments.     

Cenomanian (Upper Cretaceous) carbon isotope stratigraphy of terrestrial organic matter for the Yezo Group, Hokkaido, Japan

Abstract Carbon isotope fluctuations of sedimentary organic matter along the two geological traverses in the Yezo Group, Hokkaido, northern Japan, elucidate a detailed chemostratigraphy for the Cenomanian Stage on the northwestern Pacific margin. Visual characterization of the kerogen from mudstone samples shows that the major constituents of sedimentary organic matter originated as terrestrial higher plants. The atomic hydrogen/carbon ratios of the kerogen suggest that the original δ¹³C values of terrestrial organic matter (TOM) have not been affected significantly by thermal diagenesis. The patterns in two δ¹³C_TOM curves are similar and independent of changes in lithology and total organic carbon contents, which suggests that TOM was mixed sufficiently before the deposition in the Yezo forearc basin for the δ¹³C composition having been homogenized. In addition, this implies that the Hokkaido δ¹³C_TOM profiles represent the averaged temporal δ¹³C variations of terrestrial higher‐plant vegetation in the hinterlands of northeast Asia during Cenomanian time. Three shorter‐term (ca. 0.1 my duration) positive‐and‐negative δ¹³C_TOM fluctuations of ∼1‰ are present in the Lower to Middle Cenomanian interval in the Yezo Group. On the basis of the age‐diagnostic taxa (ammonoids, inoceramids and planktic foraminifers), these discrete δ¹³C_TOM events are interpreted to be correlated with those in the δ¹³C curves of pelagic carbonates from European basins. The correlation of δ¹³C events between the European and Yezo Group sections suggests that the shorter‐term δ¹³C fluctuations in Cenomanian ocean‐atmosphere carbon reservoirs are useful for global chemostratigraphic correlation of marine strata. In particular, the correlation of δ¹³C fluctuations of the so‐called ‘Mid‐Cenomanian event’ (MCE) implies: (i) the δ¹³C variations of global carbon reservoir during the MCE are precisely recorded in the δ¹³C_TOM records; and (ii) the MCE δ¹³C_TOM event is an efficient chronostratigraphic index for the Lower/Middle Cenomanian boundary of the Mid‐Cretaceous sequences.     

Post-seismic Effects of an M 7.2 Earthquake and Microseismicity in an Abandoned, Flooded, Deep Mine

—?An M 7.2 earthquake took place 67?km southeast of an abandoned mine containing flooded, vertical ore veins at depth of 1?km. Multidisciplinary monitoring of the unweathered country rock within the mine was carried out for a distance of about one kilometer. The M 7.2 earthquake was followed by significant post-seismic changes in strain and tilt of ～10^?6, with a self-potential of ～10?mV, and an increase in the water level in the mine of about 10?cm/day. These phenomena continued for several months before returning to pre-earthquake levels. Coseismic elastic deformations were too small to account for the observed post-seismic changes, and have different senses in strain and tilt from the observed post-seismic changes. The contractions in strain and the changes in the electric self-potential and water-elevation rate strongly suggest an increase in pore pressure. An increase in microseismic activity (M?M 7.2 earthquake.     

Physical properties of the outflow sources in taurus

We have searched for CO outflows in eight embedded IRAS sources located in the Taurus molecular cloud using the 45m telescope of Nobeyama Radio Observatory. We have detected CO wing emission in four of these sources. CO outflow associated with TMC1A (04365+2535) is strong and spatially compact (radius 0.04 pc). The dynamical timescale of 2.5 × 10³ yr suggests this outflow is the youngest one in Taurus.We have combined our data with previously published survey data and have analyzed the physical properties of the outflow sources. We found that 12 out of 16 embedded sources ( 75 %) have CO outflows associated with them; this indicates that almost all stars experience a phase of molecular outflow in their embedded stage. The IRAS color of the outflow sources suggests that the outflows appear in considerably early phase of the evolution of YSOs, that is, as early as YSOs became observable with IRAS and that visible outflow sources are in a transient phase of evolution between embedded sources and visible T Tauri stars without outflow. Visible outflow sources are systematically more luminous than visible no-outflow sources, while embedded outflow sources have comparable luminosities with visible no-outflow sources. Such luminosity function suggests that the YSOs with outflow undergo mass accretion and increase their stellar mass as they progress from embedded sources to visible outflow sources. Typical mass accretion rate derived from the bolometric luminosity is 2 ×10^–6 M _yr ^–1. The timescale for mass accretion to acquire typical stellar mass, 0.5 – 0.8M , is 2.5 – 4 × 10⁵ yr.     

Direct ascent to the surface of asthenospheric magma in a region of convex lithospheric flexure

The stress field of oceanic lithosphere controls the distribution of submarine petit-spot volcanoes. However, the eruption sites of these petit-spot volcanoes are considered to be limited to concavely flexed regions of lithosphere off the outer rise. Here, we present new data for a recently identified petit-spot lava field on a convexly flexed section of the lithosphere adjacent to the subduction zone offshore of northeast Japan in an area containing more than 80 volcanoes. This area is marked by strongly alkaline lavas that were erupted on the convexly flexed region. As for the concavely flexed region where the petit-spots previously reported, the base of the lithosphere beneath the eruption sites is under extension, whereas the upper part of the lithosphere is under compression. This change in the stress field, from the lower to upper lithosphere, causes ascending dikes to stall in the mid-lithosphere, leading to metasomatic interaction with the surrounding peridotite. The new geochemical data of rocks and xenocrysts presented in this study indicate that strongly alkaline magmas erupted on the convexly flexed region would have ascended more rapidly through the mid-depth of lithosphere because of the extensional regime of the upper lithosphere and decreasing the degree of metasomatic reaction with the surrounding mantle peridotite. The results indicate that the degree of metasomatism and the compositional variations of petit-spot magmas are controlled mainly by the stress field of the lithosphere.     

Wakefieldite‐(Nd), a New Neodymium Vanadate Mineral in the Arase Stratiform Ferromanganese Deposit,Kochi Prefecture,Japan

Wakefieldite‐(Nd), NdVO₄, is a new mineral found from the Arase stratiform ferromanganese deposit in Kochi Prefecture, Shikoku Island, Japan. It is the Nd‐dominant analogue of wakefieldite‐(Y) and wakefieldite‐(Ce). The ferromanganese ore specimen mainly consists of hematite and caryopilite, and wakefieldite‐(Nd) is typically enclosed in caryopilite. Wakefieldite‐(Nd) is tetragonal, I4₁/amd, a = 7.338(16) Å, c = 6.509(19) Å, V = 350.5(18) ų, Z = 4. The four strongest lines in the X‐ray diffraction pattern [d(Å), I/I₀, hkl] using a Gandolfi camera are (3.67, 100, 200); (2.74, 51, 112); (4.84, 27, 101) and (1.89, 25, 312). Chemical composition of wakefieldite‐(Nd) are V₂O₃ 35.25, As₂O₃ 0.93, SiO₂ 0.14, MnO 1.45, Fe₂O₃ 0.41, Y₂O₃ 2.87, La₂O₃ 7.61, Ce₂O₃ 7.37, Pr₂O₃ 6.04, Nd₂O₃ 26.79, Sm₂O₃ 4.41, Eu₂O₃ 1.36, Gd₂O₃ 3.41, Tb₂O₃ 0.22, Dy₂O₃ 1.41, Er₂O₃ 0.10, total 99.77 wt.%. The empirical formula is (Nd_0.403La_0.118Ce_0.114Pr_0.093Y_0.064Sm_0.064Mn_0.052Gd_0.048Eu_0.020Dy_0.019Fe_0.013Tb_0.003Er_0.001)_1.012(V_0.981As_0.020Si_0.006)_1.007O₄ on the basis of O = 4. The calculated density is 4.782 g/cm³. Microtexture and co‐existing relationship between wakefieldite‐(Nd) and caryopilite suggest that recrystallization and dehydration of Fe‐ and Mn‐oxyhydroxide led to the generation of hematite, caryopilite, rhodochrosite and wakefieldite‐(Nd) by the metamorphism during the accretion of the host unit of the Arase deposit. Chondrite‐normalized REE pattern of the host ferromanganese ore, which is regarded as oceanic metalliferous sediment in origin, shows negative Ce anomaly. Chemical composition of wakefieldite‐(Nd) reflects Ce‐depleted bulk composition of REE‐enriched ferromanganese ore.     

Effects of bacterial films on diatom attachment in the initial phase of marine fouling

The effects of bacterial films on diatom attachment were examined in short-term laboratory experiments, comparing the density of immotile pennate diatoms attached to plates previously coated with bacterial films (BP) with that of sterile plates (SP). The predominant diatom and bacterium wereSynedra sp. andAlcaligenes sp., respectively. There was no significant difference between densities of diatoms on SP and those on BP at each contact time (2, 5, 10, 30, 60 min), although bacterial densities on BP were 17 to 185 times those on SP. Moreover, SEM observations showed that the percentage of diatoms in contact with bacteria on SP were quite low. The results of this study indicate that a bacterial film is not always necessary for diatoms to attach to substrata and that the film ofAlcaligenes sp. had no effect on the attachment ofSynedra sp.     

Safety Management Based on Detection of Possible Rock Bursts by AE Monitoring during Tunnel Excavation

Summary. As underground development continues at great depths, the danger of rock bursts will inevitably increase. It is important to consider countermeasures for avoiding rock bursts in underground work. In Japan, rock bursts have actually been experienced during construction of several tunnels, including the Kan-etsu tunnel construction project, during which many rock bursts were observed. Stress analysis of tunnels is performed based on initial stress measurements in the base rock. In addition, AE measurement has been adopted in construction management, allowing safer excavation. Collective analysis of the data obtained has been shown to be effective for safety control during excavation of hard base rock. In this research, initial stress measurements in base rock, secondary stress analysis around a tunnel, and AE measurements are examined. Based on the results of this analysis, it is clear that the generation of rock bursts is related to the presence of geological discontinuities.