Kinetics of nitroreductase-mediated phytotransformation of TNT in vetiver grass

The effectiveness of vetiver grass in removing 2,4,6-trinitrotoluene (TNT) and the involvement of nitroreductase (NR) enzyme in TNT degradation in vetiver has been documented in our earlier studies. The present study was designed to optimize the parameters influencing NR enzyme-mediated TNT transformation in vetiver. The current study is the first report of detailed kinetic examination of NR enzyme induced by TNT in vetiver. Results show that NR activity in vetiver root increased with exposure time as well as with initial TNT concentration, showing a first-order reaction kinetics at lower and a second-order reaction kinetics at higher TNT concentrations. Nitroreductase activity was higher in shoot compared to root in all TNT treatments. Increasing concentration of TNT resulted in a significant increase in the NR activity in shoot. Very high increase in the shoot NR activity indicates a faster root-to-shoot translocation of TNT. The optimum range of the factors influencing NR-mediated TNT transformation and the kinetic parameters were determined, which will be crucial for the application of vetiver for phytoremediation of TNT-contaminated systems.     

A simplified fragility analysis of fan type cable stayed bridges

A simplified fragility analysis of fan type cable stayed bridges using Probabilistic Risk Analysis (PRA) procedure is presented for determining their failure probability under random ground motion. Seismic input to the bridge support is considered to be a risk consistent response spectrum which is obtained from a separate analysis. For the response analysis, the bridge deck is modeled as a beam supported on springs at different points. The stiffnesses of the springs are determined by a separate 2D static analysis of cable-tower-deck system. The analysis provides a coupled stiffness matrix for the spring system. A continuum method of analysis using dynamic stiffness is used to determine the dynamic properties of the bridges .The response of the bridge deck is obtained by the response spectrum method of analysis as applied to multidegree of freedom system which duly takes into account the quasi - static component of bridge deck vibration. The fragility analysis includes uncertainties arising due to the variation in ground motion, material property, modeling, method of analysis, ductility factor and damage concentration effect. Probability of failure of the bridge deck is determined by the First Order Second Moment (FOSM) method of reliability. A three span double plane symmetrical fan type cable stayed bridge of total span 689 m, is used as an illustrative example. The fragility curves for the bridge deck failure are obtained under a number of parametric variations. Some of the important conclusions of the study indicate that (i) not only vertical component but also the horizontal component of ground motion has considerable effect on the probability of failure; (ii) ground motion with no time lag between support excitations provides a smaller probability of failure as compared to ground motion with very large time lag between support excitation; and (iii) probability of failure may considerably increase for soft soil condition.     

Magnetopause boundary structure deduced from the high-time resolution particle experiment on the Equator-S spacecraft

An electrostatic analyser (ESA) onboard the Equator-S spacecraft operating in coordination with a potential control device (PCD) has obtained the first accurate electron energy spectrum with energies &7 eV-100 eV in the vicinity of the magnetopause. On 8 January, 1998, a solar wind pressure increase pushed the magnetopause inward, leaving the Equator-S spacecraft in the magnetosheath. On the return into the magnetosphere approximately 80 min later, the magnetopause was observed by the ESA and the solid state telescopes (the SSTs detected electrons and ions with energies &20–300 keV). The high time resolution (3 s) data from ESA and SST show the boundary region contains of multiple plasma sources that appear to evolve in space and time. We show that electrons with energies &7 eV–100 eV permeate the outer regions of the magnetosphere, from the magnetopause to &6Re. Pitch-angle distributions of &20–300 keV electrons show the electrons travel in both directions along the magnetic field with a peak at 90° indicating a trapped configuration. The IMF during this interval was dominated by Bx and By components with a small Bz.     

Application of criteria and indicators in community based sustainable mangrove management in the Sunderbans,India

Management of mangroves in a sustainable ecological as well as economic way is a difficult proposition and needs multi-disciplinary intervention. This paper highlights the importance of criteria and indicators to evaluate the status of performance of communities in managing their mangroves sustainably. The framework developed on the basis of 6 principles, 12 criteria, 36 indicators and 47 verifiers had been applied for assessment of Eco-Development Committees of the Sunderbans, India. Results show the presence of a highly positive correlation between performance of the committees and condition of the mangrove environment. Level of implementation of legal and institutional management strategies to protect mangroves proves to be the essential pre-condition in achieving sustainability. The study further demonstrates the effectiveness of the developed framework in the context of community forestry since the methodology was designed to be simple and flexible so as to apply it in forest management unit level without any major difficulty.     

Pressure Energy Diffusion Rates in the Wall-Wake Region of Immobile Solid Sphere in Open Channel Flows-Numerical Simulation and Experimental Study

Water Resources - Present study elucidates the near-bed pressure energy diffusion rates in the wall-wake region of flowpast a stationary solid sphere in open channel. Vertical distributions of...     

Ground motion amplification by cylindrical valleys embedded in a layered medium

The phenomenon of localized amplification of seismic waves due to subsurface irregularities has attracted the attention of seismologists and engineers for many years. In this paper we present a hybrid numerical method to investigate the ground motion amplification due to sedimentary basins. This method combines the integral representation and the finite element to solve the scattering problem due to an arbitrary cylindrical scatterer. A study is done on two shapes of valleys to show the effect of the angle of incidence, the incident wave type and the layering on the amplification of the surface displacement components by different valleys.     

Palaeoclimate,stratigraphy and prehistory in north and west Rajasthan

Quaternary formations in western Rajasthan are of fluvial, locustrinal and aeolian origins. Fossil gravel ridge of Jayal in Nagaur District is one of the earliest quaternary formations so far recorded in the region. A rich lower palaeolithic habitation-cum-workshop site has been discovered in association with the gravel ridge. Multiple evidence for climatic change in the area during the quaternary period is discussed.     

Effects of the Herbicide Diuron on Cordgrass (<Emphasis Type="Italic">Spartina foliosa</Emphasis>) Reflectance and Photosynthetic Parameters

Early indicators of salt marsh plant stress are needed to detect stress before it is manifested as changes in biomass and coverage. We explored a variety of leaf-level spectral reflectance and fluorescence variables as indicators of stress in response to the herbicide diuron. Diuron, a Photosystem II inhibitor, is heavily used in areas adjacent to estuaries, but its ecological effects are just beginning to be recognized. In a greenhouse experiment, we exposed Spartina foliosa, the native cordgrass in California salt marshes, to two levels of diuron. After plant exposure to diuron for 28 days, all spectral reflectance indices and virtually all fluorescence parameters indicated reduced pigment and photosynthetic function, verified as reduced CO₂ assimilation. Diuron exposure was not evident, however, in plant morphometry, indicating that reflectance and fluorescence were effective indicators of sub-lethal diuron exposure. Several indices (spectral reflectance index ARI and fluorescence parameters EQY, Fo, and maximum rETR) were sensitive to diuron concentration. In field trials, most of the indices as well as biomass, % cover, and canopy height varied predictably and significantly across a pesticide gradient. In the field, ARI and Fo regressed most significantly and strongly with pesticide levels. The responses of ARI and Fo in both the laboratory and the field make these indices promising as sensitive, rapid, non-destructive indicators of responses of S. foliosa to herbicides in the field. These techniques are employed in remote sensing and could potentially provide a link between landscapes of stressed vegetation and the causative stressor(s), which is crucial for effective regulation of pollution.