全球增暖对甲烷生物圈源的影响及其气候后果

1.引言人们认为工业活动对全球气候的影响主要是二氧化碳的温室效应。近来,已经提出在北半球观测到的可能是由于人类活动产生的较高的臭氧浓度会造成气候增温0.2 K(Fishman等,1979)。而且,在气候-化学耦合     

雷达气象学在八十年代的发展

雷达气象学是一门实验科学,它是从实验的角度提出科学问题,其进展在很大程度上依赖于技术的发展和技术工艺的状况。所以雷达气象学的未来应该从技术上或者从科学的前景方面来加以探讨。通常是倾向于先考虑科学上某些有意义的领域,再提出技术要求,以达到科学问题的实现。一、现状从七十年代开始,多普勒雷达才开始作为一种可行的定量研究工具而出现,由于雷达资料处理技术的发展和理论研究,才使多普勒雷达测量技术建立在坚实的基础上,并解决了许多有关估计径向速度的精度问题。到目前为止,根据它在科学应用上     

澳大利亚前寒武系和寒武系迭层石的分布

前言我们在澳大利亚工作的目的是检验苏联迭层石分类及其分带方案(Scheme)的可靠性。我们发现他们的分类,如在别处讨论过的一样,在我们的迭层石中一般是可应用的。我们尽可能广泛地获得了迭层石在地理和时代(temporal)上分布范围的资料。普里斯(1971)对阿得累德(Adelaide)地槽前寒武纪和寒武纪迭层石作了详细的地层和岩相研究。沃尔特(1970,1972)研究了阿马德斯(Amadeus)、乔治纳(Georgina)、麦卡阿瑟     

Study on the Free Radical Scavenging Activity of Sea Cucumber(Paracaudina chinens var.) Gelatin Hydrolysate

Gelatin from the sea cucumber(Paracaudina chinens var.) was hydrolyzed by bromelain and the hydrolysate was found to have a high free radical scavenging activity. The hydrolysate was fractionated through an ultrafiltration membrane with 5 kDa molecular weight cutoff(MWCO). The portion(less than 5 kDa) was further separated by Sephadex G-25. The active peak was col-lected and assayed for free radical scavenging activity. The scavenging rates for superoxide anion radicals(O2·-) and hydroxyl radi-cals(·OH) of the fraction with the highest activity were 29.02% and 75.41%,respectively. A rabbit liver mitochondrial free radical damage model was adopted to study the free radical scavenging activity of the fraction. The results showed that the sea cucumber gelatin hydrolysate can prevent the damage of rabbit liver and mitochondria.     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

HYDROPOWER RESOURCES IN UPPER YANGTZE

ＨＹＤＲＯＰＯＷＥＲＲＥＳＯＵＲＣＥＳＩＮＵＰＰＥＲＹＡＮＧＴＺＥＣＨＥＮＪｉｓｈｅｎｇ（ＤｉｒｅｃｔｏｒＥｍｅｒｉｔｕｓ，ＹａｎｇｔｚｅＲｉｖｅｒＳｃｉｅｎｔｉｆｉｃＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅ（ＹＲＳＲＩ），２３ＨｕａｎｇｐｕＲｏａｄ，Ｗｕ...     

岩浆流体对冲绳海槽海底成矿热水系统的可能贡献

海底火山活动区成矿热液流体来源长期存在争议,尽管海水来源说更为流行,但本文提供的下列证据表明,来自长曲质岩浆的流体对冲绳海槽现代活动热水成矿系统有较大贡献：（１）冲绳海槽ＪＡＤＥ热水区出现异常高热流,其下方１￣２ｋｍ处存在浅位长英质岩浆房;（２）ＣＯ２、烃类、Ｈ２等气体在热水成矿体系中极度富集甚至形成独立气流,与热水流体共存。气体的碳、氦同位素资料表明其来自长英质岩浆本系;（３）ＪＡＤＥ成矿热水系     

在世界包围中的新西兰农业:当今政策的调整

在这篇文章里,我研究了十九世纪世界贸易和殖民扩展中的新西兰农业发展,继而研究了导致农业出口依赖于世界贸易、技术和动力平衡所出现的经济过程。我调查了农业(1963—1984年)近期的竞争,以及转向调整和出现二十世纪经济和剩余农业的含意。     

SHORT COMMUNICATION COMMENTS ON THE RESIDUAL BILINEARIZATION METHOD

Through theoretical analysis and computer simulation,this short communication comments on theresidual bilinearization(RBL)method and compares it with non-bilinear rank annihilation(NBRA)forthe treatment of second-order calibration with non-bilinear data.It is found that these two methods aremathematically equivalent but have different noise propagation properties.The second-order advantage,namely quantitation in the presence of unknown interferences,can be carried over to non-bilinear dataonly if there exists a net analyte rank(NAR)for the analyte of interest.