Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

The Effect of Temperature on Growth and Energy Budget of the Polychaete,Neanthes japonica Izuka

Growth and energy budget of the polychaete, Neanthesjaponica, at various temperatures (17, 20, 23, 26 and 29 ℃) were investigated in this study. The growth, as indicated by final dry weight and specific growth rate (SGR), increased with increasing temperature, with the maximum level at 26℃, and then decreased significantly at 29℃. A similar trend was observed in feeding rate, food conversion efficiency (FCE) and apparent digestive rate (ADR). However, no significant differences were detected in ADR among all the temperature treatments. In the pattern of energy allocation, faeces energy was only a small component of energy budget and had little influence on the proportion of food energy allocated to growth. The metabolic energy accounted for a large portion of energy intake for each temperature treatment. The nitrogen excretion was appreciable with changing temperature. The two expendi-ture terms (respiration energy and excretion energy) in energy budget were the major factors influencing the proportion of food en-ergy allocated to growth. These results revealed that temperature affected the growth of N. japonica mainly by influencing feeding rate and FCE. In addition, regression equations describing the relationship between feeding rate, faecal production, SGR, FCE and temperature were obtained. The optimum temperatures for feeding rate, FCE and SGR were estimated at 25.01 ℃, 24.24℃ and 24.73 ℃, respectively, from the regression equations.     

Enzymatic activity in the rhizosphere of Spartina maritima: potential contribution for phytoremediation of metals

Extracellular enzymatic activity (EEA) of five enzymes (peroxidase, phenol oxidase, beta-glucosidase, beta-N-acetylglucosaminidase and acid phosphatase) was analysed in sediments colonised by Spartina maritima in two salt marshes (Rosário and Pancas) of the Tagus estuary (Portugal) with different characteristics such as sediment parameters and metal contaminant levels. Our aim was a better understanding of the influence of the halophyte on microbial activity in the rhizosphere under different site conditions, and its potential consequences for metal cycling and phytoremediation in salt marshes. Acid phosphatase and beta-N-acetylglucosaminidase presented significantly higher EEA in Rosário than in Pancas, whereas the opposite occurred for peroxidase. This was mainly attributed to differences in organic matter between the two sites. A positive correlation between root biomass and EEA of hydrolases (beta-glucosidase, beta-N-acetylglucosaminidase and acid phosphatase) was found, indicating a possible influence of the halophyte in sediment microbial function. This would potentially affect metal cycling in the rhizosphere through microbial reactions.     

基于不同轨道高度iHCO通信卫星的CAPS星座优化研究

基于通信卫星的导航系统可以利用比地球静止轨道（Geostationary Earth Orbit,GEO）高约200 km的倾斜高圆轨道（inclined Highly Circular Orbit,iHCO）通信卫星组成导航星座.结合两种轨道高度的倾斜高圆轨道通信卫星,仿真分析了利用倾斜高圆轨道卫星组成的中国区域定位系统（Chinese Area Positioning System,CAPS）的导航性能,并讨论了利用倾斜高圆轨道卫星组成的中国区域定位系统实现中国区域覆盖的最佳星座布局.     

New CO and Millimeter Continuum Observations of the z = 2.394 Radio Galaxy 53W002

The Chandra X-Ray Observatory observed the Crab Nebula and pulsar during orbital calibration. Zeroth-order images with the High-Energy Transmission Grating (HETG) readout by the Advanced CCD Imaging Spectrometer spectroscopy array (ACIS-S) show a striking richness of X-ray structure at a resolution comparable to that of the best ground-based visible-light observations. The HETG-ACIS-S images reveal, for the first time, an X-ray inner ring within the X-ray torus, the suggestion of a hollow-tube structure for the torus, and X-ray knots along the inner ring and (perhaps) along the inward extension of the X-ray jet. Although complicated by instrumental effects and the brightness of the Crab Nebula, the spectrometric analysis shows systematic variations of the X-ray spectrum throughout the nebula.     

Wind and freshwater influence over hydrocarbon dispersal on Patos Lagoon, Brazil

The two-dimensional barotropic, hydrodynamic and transport model MOHID is applied to the Patos Lagoon system using a nested modelling approach to reproduce both the lagoon and estuary hydrodynamics. A new Lagrangian oil spill model is presented and used to simulate a hypothetical oil spill in the estuary. Hydrodynamic fields are validated and used to force the oil model. Results show that the hydrodynamics of this system is mainly controlled by the wind and freshwater discharge. The dispersion, concentration and thickness evolution of the oil in the first day after the spill is determined by the equilibrium between these two factors. The freshwater discharge is the major factor controlling the oil dispersion for discharges greater than 5000 m3 while the wind assumes control for lower discharge amounts. The results presented are a first step toward a coastal management tool for the Patos Lagoon.     

Possible ion-acoustic soliton formation in the ionospheric perturbations observed on DEMETER before the 2007 Pu’er earthquake

The data of ionospheric perturbations observed on DEMETER before the 2007 Pu’er earthquake are analyzed. The three-component plasma (ions, electrons and heavy ions) is studied in the fluid concept. The linear dispersion relation for ion-acoustic wave is found in the presence of heavy ions. The nonlinear dynamics is studied for arbitrary amplitude of the wave. The Sagdeev potential is calculated, which shows that solitary structure exists for Mach number within a range defined by the presence of heavy ions. ...     

中国城市(包括辖县)的工业职能分类——理论、方法和结果

在城市发展的经济基础理论的指导下,作者提出城市工业职能的概念包括城市的专业化部门、职能强度和职能规模三个要素。城市工业职能分类就是按照上述三要素的相似性和差异性对城市进行分类。本研究曾采用主因素分析、聚类分析和纳尔逊的统计分析等多种方法。最后以沃德误差法的聚类分析结果作为分类的基础,稍加修正,并以纳尔逊法分析结果作补充。中国1984年的295个城市被分成三个大类、十九个亚类和五十四个职能组。