Measuring and modelling temporal trends of 226Ra in waters of a Spanish estuary affected by the phosphate industry

The presence and temporal evolution (1990-2001) of (226)Ra in a tidal estuary affected by the phosphate industry has been investigated. Water samples collected in the course of four separate sampling campaigns were analysed for (226)Ra content using a gas flow proportional counter following Ba coprecipitation. Two (226)Ra sources have been identified: direct discharges from the industrial complex and run-off from a phosphogypsum pile. Although activity levels are similar, or even higher, than those found in other environments affected by the phosphate industry, there has been a general decrease in contamination since direct discharges ceased in 1998 due to new regulations from the EU. However, sediments are now acting as a source of Ra to the water column due to redissolution processes. A numerical model of the estuary has been developed to describe quantitatively the experimental results. The model solves the hydrodynamics and the dispersion equation of (226)Ra including interactions with sediments. Model results are, in general, in good agreement with observations.     

Patterns of heritability of decreased EROD activity and resistance to PCB 126-induced teratogenesis in laboratory-reared offspring of killifish (Fundulus heteroclitus) from a creosote-contaminated site in the Elizabeth River,VA, USA

Killifish (Fundulus heteroclitus) from a highly contaminated site on the Elizabeth River are resistant to the acute toxicity and the cytochrome P4501A (CYP1A)-inducing activity of both the sediments from the site and chemically pure polycyclic aromatic hydrocarbons (PAHs). These effects are highly heritable for one generation, but heritable to a lesser degree by subsequent generations, in clean conditions in the laboratory. We show that offspring of this population of Elizabeth River killifish are also resistant to the teratogenicity and P4501A-inducing activity of PCB congener 126, a prototypical coplanar halogenated aromatic hydrocarbon (HAH). Furthermore, the pattern of greater resistance to acute toxicity and P4501A-inducing activity in the first generation and less in subsequent generations is also observed upon exposure to PCB-126.     

一个斜压海洋环流数值模式及其数值试验

为研究大尺度海洋环流和一些大尺度海洋物理过程,我们建立了一个无海底地形的三层斜压海洋环流数值模式。模式方程采用了静力近似和Boussinesq近似,在上边界采用了钢盖近似。同时,还做了两个数值试验:第一个模拟试验,海洋从静止状态开始,以4个季节的气候平均风应力和海表大气季节平均温度为强迫条件,积分区城选择45℃以北的太平洋,数值积分了5个模式年,这时海洋的上层环流和温度的年变化趋于平稳。计算结果再现了太平洋气候平均状态的海温分布和主要海流的大尺度特征以及它们的年变化特征。如西部强化流、赤道潜流等。第二个数值试验,在第一个数值模拟的基础上给定赤道西太平洋一个风应力异常区,数值积分了几个月。从计算结果中可看到类似E1 Ni■o的现象,并且初步地分析了赤道波动在E1 Ni■o形成过程中的作用。     

大西洋中脊的热液矿泉

在过去10年中，通过国际合作，对大西洋中肯的热液矿泉及周围地质背景进行了研究。TAG热液矿泉位于26°N，45°W，距大西洋中脊轴数公里，它是世界卜已发现的最大热液矿泉之一．这个活动的热泉位于一直径为10m的烟囱上，该烟囱位于一直径为150－200m、高40m的椭圆形硫化矿山上．在这个硫化矿山附近几公里范围内还发现了几个不活动的热泉。TAG靠近两条转换断层之间的大西洋中脊．区域重力调查表明，TAG北部的剪切带在向北漂移．分析TAG海底玄武岩表明，源岩在10－15km深处．从旁侧声纳资料看出，TAG由两个复杂的烟囱组成．近海底地球…     

上海全面完成海岛资源综合调查

上海市海岛资源综合调查已于不久前全面完成,调查中所获得的资料与成果业已进入开发应用。上海市海岛资源综合调查领导小组近日召开的“上海市海岛资源综合调查暨开发试验先进表彰大会”透露,上海市完成的这项达到国际先进水平的重大基础研究成果,为上海市继跨越苏州河、黄浦江后,正在跨越长江的     

LARGE-SCALE CIRCULATION CONDITIONS AFFECTING THE HURRICANE FORMATION OVER THE NORTH ATLANTIC OCEAN

A contrasting study of the large-scale circulation features responsible for months or seasons with many hurricanes and those with few hurricanes has revealed that the frequency of hurricane formation over the North Atlantic Ocean is enhanced by the following conditions:There exists a well-developed subtropical high-pressure belt displaced to the north; a deep Icelandic low; a stronger-than-normal polar vortex with a deep polar trough dipping far southward along the eastern part of the United States; a dominant high-pressure ridge over western Canada and the United States; and a jet stream displaced northward over these regions. At 200 hPa, an anomalous anticyclonic circulation prevails over the subtropical North Atlantic. A stronger-than-normal upper easterly flow prevails. Positive sea surface temperature anomalies prevailing on a seasonal or long-term basis tend to be favorable for the formation of hurricanes.     

沙蚕投喂池养虾技术

１沙蚕的主要用途用沙蚕养殖对虾的研究成果早已推广，且在不少地方取得了节约饵料１０～４０％、提高产量３０～３５％的较好效果［２，３］。但是，由于沙蚕自身进行新陈代谢需要耗氧，且具杂食的习性．因此，有时也发生争夺饵料，出现缺氧。在用沙蚕养虾的过程中，沙蚕和对虾之间的关系并非固定不变，沙蚕也并不总是处于被对虾捕食的地位。近年有不少地方，由于用沙蚕养虾，出现对虾小苗成活率极低，甚至全无的现象。在文登市销售的虾苗中发现，在同一批虾苗中，只是由于买主不同，虾苗成活率高低相差悬殊。分析发现：凡是虾苗成活率低的…     

Evaluation of acetylcholinesterase activity in several zooplanktonic crustaceans

Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species.     

An aromatase inhibitor and tamoxifen decrease plasma levels of o,p'-DDT and its metabolites in Nile tilapia (Oreochromis niloticus)

The objective of this study was to determine if tamoxifen or an aromatase inhibitor (4-hydroxyandrostenedione; 4-OHA) affected plasma concentrations of o,p' -DDT and its metabolites, o,p'-DDD and o,p'-DDE, in mature tilapia. Male and female tilapia were injected 6 times intraperitoneally with o,p'-DDT (40 mg/kg) alone or in combination with 4-OHA (2 mg/kg) over a 12 day period. An additional group of male fish was injected with tamoxifen (5 mg/kg) plus o,p'-DDT. At the end of the treatment period, plasma samples were extracted and analyzed by GC/ECD. Females injected only with o,p'-DDT had significantly higher levels of o,p'-DDT compared with males. Interestingly, females and males treated concomitantly with o,p'-DDT and 4-OHA or tamoxifen had significantly lower concentrations of plasma o,p'-DDT (about 50%) compared with fish treated with only o,p'-DDT. These initial results suggest that an interaction between endocrine-active compounds occurs in vivo in tilapia and may involve alterations in metabolism of o,p'-DDT.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.