海底天然气水合物地球化学勘探新技术

天然气水合物是目前寻找新型替代能源的热点之一，其勘探、开发和利用的科学与技术是当前面临的重大课题。除地球物理方法可对天然气水合物定位外，地球化学勘探也是寻找它的重要手段。天然气水合物的几种地球化学勘探方法分别利用其直接标志和间接标志。直接标志包括烃类气体含量测定等；间接标志包括孔隙水离子浓度异常、同位素地球化学异常、标志性矿物(标型矿物)及沉积物热释光分析等方法。     

热带西太平洋对风应力的斜压响应

发展了一个线性热带西太平洋两层模式,分别为混合层和温跃层,其密度、温度各不相同.利用这一模式分析了热带西太平洋对纬圈风应力的响应,求出西边界的解,以及解析地求得热带西太平洋温跃层厚度、洋流及海温分布.结果表明,热带西太平洋物理量的变异在El Nino/La Nina事件中起着重要作用,在温跃层中海温变化的振幅明显大于混合层,这从理论上支持了近年来的观测事实.     

基于OpenGL的DEM三维仿真方法研究

本文介绍了如何利用VC 结合OpenGL实现DEM三维仿真,其中重点介绍了OpenGL的主要功能、DEM模型、VC 中OpenGL的参数设置、高程数据的读取、地形的仿真。最后实现了DEM三维模型的缩放旋转、投影变换、地貌信息、空间查询、人机交互等功能。     

立式储油罐地震作用下的抗提离可靠度分析

储油罐在水平地震作用下会出现罐底提离而导致储油罐发生提离破坏,影响储油罐的可靠性。为了研究立式储油罐在地震作用下的抗提离可靠度情况,文中总结建立了在储油罐抗提离可靠度分析中涉及的随机变量的概率模型,运用可靠性分析的JC法对不同工况下的储油罐进行了抗提离可靠度计算,得出了储油罐在地震作用下的提离破坏规律。通过计算实例可以看出,对大型储油罐在地震作用下的抗提离可靠度分析是切实可行的。     

Variations in retention efficiency of bivalves to different concentrations and organic content of suspended particles

Retention efficiencies (RE) of scallop (Chlamys farreri),oyster (Crassostrea gigas) and mussel (Mytilus edulis) in a flow-through system were measured to understand the short-term response to various particle and organic matter concentrations.By comparing the RE of C.farreri with that of C.gigas and M.edulis,we gained further knowledge on the feeding physiological characteristics of C.farreri and ascertained the possible cause of high summer mortalities of this species.The experimental feeding conditions in...     

Cloning,characterization,and expression analysis of the DEAD-box family genes,Fc-vasa and Fc-PL10a,in Chinese shrimp (Fenneropenaeus chinensis)

RNA helicases of the DEAD-box and related families are involved in various cellular processes including DNA replication,DNA repair,and RNA processing.However,the function of DEAD-box proteins in aquaculture species is poorly understood at molecular level.We obtained the full-length cDNA sequences of two genes encoding helicase-related proteins,Fc-vasa and Fc-PL10a,from the testes of Chinese shrimp,Fenneropenaeus chinensis.The two predicted amino acid sequences contain all the conserved motifs characterized ...     

Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain.This prtV gene encodes a putative protein of 918 amino acids,and is highly homologous to the V.cholerae prtV gene.We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar,lower protease activity against azocasein,and lower activity for four glycosidases.This prtV mutant strain also had increased activity for two esterases in its extracellular products,as analyzed by the API ZYM system.In additi...     

Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)

Lymphocystis disease,caused by the lymphocystis disease virus (LCDV),is a significant worldwide problem in fish industry causing substantial economic losses.In this study,we aimed to develop the DNA vaccine against LCDV,using DNA vaccination technology.We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate.The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line.The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also ana...     

Antithrombotic effects of bromophenol,an alga-derived thrombin inhibitor

Thrombin,the ultimate proteinase of the coagulation cascade,is an attractive target for the treatment of a variety of cardiovascular diseases.A bromophenol derivative named (+)-3-(2,3-dibromo-4,5-dihydroxy-phenyl)-4-bromo-5,6-dihydroxy-1,3-dihydroiso-benzofuran 1,isolated from the brown alga Leathesia nana exhibited significant thrombin inhibitory activity.In this study,we investigated the inhibition of human thrombin in vitro with this bromophenol derivative,and its antithrombotic efficacy in vivo using th...     

Morphological Comparison between Chinese Ayu and Japanese Ayu and Establishment of Plecoglossus altivelis chinensis Wu&Shan subsp.nov.

Ayu(Plecoglossus altivelis)belongs to Osmeri-formes,Qsmeridae,Plecoglossus.It is small-sized,one-year living species with high economic value.It can befound in China,Japan,the Peninsula of Korea,andthe north of Vietnam.Nishida(1988)classifiedJapanese native Ayu into two subspecies:nominatesubspecies(Plecoglossus altivelis altivelis)mainlydistributed in the mainland of Japan,the peninsula ofKorea,Taiwan and China mainland;Ryukyus sub-species(Plecoglossus altivelis ryukyuensis)only dis-tr…