BTEX anomalies used as indicators of submarine oil and gas reservoirs

It is a conventional method for petroleum prospecting to generally use paraffin hydrocarbon as basic indexes of oil and gas. This conventional geochemical technology, however, shows some limits in the prospecting as paraffin is vulnerable to influences from human and biologic activities. Consequently, BTEX (short for benzene, toluene, ethyl benzene and xylem, which are direct biomarkers) among aromatic hydrocarbon series has been taken into account for the oil and gas prediction. Domestic and foreign study results demonstrate that BTEX is hardly disturbed and can well indicate oil and gas reservoirs. Based on measured data from a South China Sea area, the present authors have used self-developed visual assessment software for petroleum prospecting has been used to process data, strip background anomalies, and outline significant BTEX anomalies. By comparison with stratigraphic profiles of the target area, it is confirmed that BTEX is a good indication of marine oil and gas during the petroleum prospecting.     

The application of hyperspectral remote sensing to coast environment investigation

Requirements for monitoring the coastal zone environment are first summarized. Then the application of hyperspectral remote sensing to coast environment investigation is introduced, such as the classification of coast beaches and bottom matter, target recognition, mine detection, oil spill identification and ocean color remote sensing. Finally, what is needed to follow on in application of hyperspectral remote sensing to coast environment is recommended.     

养殖大菱鲆细菌性红体病病原菌的分离与鉴定

从患有红体病的养殖大菱鲆(Scophthalmus maximus)的肌肉中分离得到一株优势菌并记为H1.经人工注射感染证实H1即为引起养殖大菱鲆红体病的病原菌,其半致死量LD50为2.82×105CFU/mL,而低浓度(1.41×103 CFU/mL)没有造成死亡,但注射部位有脓肿现象,注射相同体积1.5%无菌生理盐水的对照组没有明显的症状,注射部位也无异常.革兰氏染色显示该菌为革兰氏阴性,菌体呈杆状,周生鞭毛.综合该菌的形态、常规生理生化特征和API32E鉴定结果,发现H1与迟钝爱德华氏菌的表性特征非常相似,相似率迭到99.9%.在分子水平上对其16SRNA基因序列的测定,同源性分析,结果表明H1与迟钝爱德华氏菌的亲缘关系最近,相似度达到99%.综合上述研究结果,将该菌株初步鉴定为迟钝爱德华氏茵(Edwardsiella tarda).     

莱州湾南岸海水入侵现状评价

根据莱州湾南岸海水入侵区地下水水质特点,将海水入侵等级划分为4级。同时,选取Cl^-,矿化度,SO4^2-,rHCO3^-/rCl^-,K^-等指标作为评价因子,并分别确定了这5项指标的等级划分范围。然后,运用模糊数学的综合评判方法,对研究区内海水入侵现状做出评价。     

Sensitivity of penman-monteith reference crop evapotranspiration in Tao’er River Basin of northeastern China

A non-dimensional relative sensitivity coefficient was employed to predict the responses of reference crop evapotranspiration （ET0） to perturbation of four climate variables in Tao＇er River Basin of the northeastern China. Mean monthly ET0 and yearly ET0 from 1961 to 2005 were estimated with the FAO-56 Penman-Monteith Equation. A 45-year historical dataset of average monthly maximum/minimum air temperature, mean air temperature, wind speed, sunshine hours and relative humidity from 15 meteorological stations was used in the analysis. Results show that： 1） Sensitivity coefficients of wind speed, air temperature and sunshine hours were positive except for those of air temperature of Arxan Meteorological Station, while those of relative humidity were all negative. Relative humidity was the most sensitive variable in general for the Tao＇er River Basin, followed by sunshine hours, wind speed and air temperature. 2） Similar to climate variable, monthly sensitivity coefficients exhibit large annual fluctuations. 3） Sensitivity coefficients for four climate variables all showed significant trends in seasonal/yearly series. Also, sensitivity coefficients of air temperature, sunshine hours and wind speed all showed significant trends in spring. 4） Among all sensitivity coefficients, the average yearly sensitivity coefficient of relative humidity was highest throughout the basin and showed largest spatial variability. Longitudinal distribution of sensitivity coefficients for air temperature, relative humidity and sunshine hours was also found, which was similar to the distribution of the three climate variables.     

东营凹陷南斜坡沙四段高频沉积旋回对滩坝砂体的沉积控制作用

在东营凹陷南斜坡的沙四段地层中,常发育典型的湖相滩坝砂体沉积.详细观察表明,这些滩坝砂体在垂向上常发育较为对称的高频基准面上升半旋回和下降半旋回,且对滩坝砂体的沉积过程有明显的控制作用.通过测井曲线的频谱分析,高频基准面的变化与米兰柯维奇轨道气候周期有着密切的关系,而单砂体规模的滩坝砂体在平面展布上又有着明显的对称性.这些都表明滩坝砂体主要沉积在湖平面升降转换的相对稳定期,同生断层活动产生的陡坎或隆起会有助于滩坝砂体的富集.可根据滩坝砂体的垂向沉积序列和组合方式来判断滩坝主体的分布位置.     

冲绳海槽热液柱动力过程的数值模拟

本文以冲绳海槽伊平屋北部热液区(126o53.80′,27o45.50′)的现场水文数据作为背景条件,使用k-ε湍流模型模拟热液柱的动力过程。模拟计算得到的羽流速度、温度和湍流耗散率等基本物理量展现了热液柱的时空演化过程。模拟结果显示,羽流最大上升高度及中性浮力面高度与海底的距离分别为83.62m和68.97m,和2014年先导专项在此附近热液区所观测的温度异常和盐度异常的深度位置(离海底约66—86m)接近。羽流的上升速度满足高斯分布,其半径b与距喷口高度z-H成正比:b=0.0985(z-H),其中z为距海底高度,H为热液烟囱体的高度。羽流的最大体积通量比喷口的初始值增加了878倍,达1.034m~3/s;在中性浮力面位置附近,动量通量达到最大值,为0.156m~4/s~2,比初始值增加了882倍;浮力通量在中性浮力面以下和BM2000(Bloomfield et al,2000)理论模型符合良好,在中性浮力面以上则呈现随高度先增加后减小的特征。本文计算得到的平均卷挟率为α≈0.0807,与背景流较弱的热液区的声学现场观测结果相符。     

饵料中添加锰对中国对虾的影响

报道了在以花生饼、鱼粉、玉米粉和麸皮为基本原料,加入不同浓度梯度的锰所配制的人工饵料饲喂中国对虾(Penaeus chinensis),对其增长、增重和存活率均无明显的影响,但添加适量的锰对其肝胰脏申的羧肽酶A具有激活作用。据此认为,利用上述原料生产的配合饵料可以不添加锰,但饵料中即使含锰量较高,对对虾的生长、成活也没有害处。     

三维含拓扑地质剖面三角剖分可视化研究

三维剖面是地质对象表达和地质问题分析中最基本和最常用的数据。该文分析三维复杂地质折剖面可视化存在的问题,并提出展开剖分算法。该方法保证了各点之间的水平距离与剖面拓扑关系不变,能够对由"多边形-弧段-结点"矢量结构构成的三维折剖面进行有效剖分。在此基础上实现了三维剖面的可视化,尤其合理解决了纹理映射中的纹理变形与重复接缝等问题。     

大弹涂鱼(Boleophthalmus pectinirostris) Gal-8L基因序列及其细菌凝集活性的鉴定

半乳糖凝集素-8基因(galectin-8,Gal-8)属于凝集素家族一员。已知Gal-8在哺乳动物的先天免疫中发挥重要作用,然而在鱼类中却鲜有报道。本研究通过转录组测序获得大弹涂鱼(Boleophthalmus pectinirostris)半乳糖凝集素-8样基因(galectin-8 like,Bp Gal-8L)c DNA序列。首先确定其序列特征,其次采用实时定量PCR(real-time quantitative PCR,q PCR)的方法鉴定Bp Gal-8L组织表达分布及其与迟缓爱德华氏菌(Edwardsiella tarda)感染相关性;最后分别通过原核表达获得N端和C端CRD区重组蛋白Bp Gal-8L-N和Bp Gal-8L-C,并采用玻片法观察这两个CRD区的细菌凝集活性和糖类结合特性。多重序列比对显示,Bp Gal-8L蛋白含有两个糖识别结构域(carbohydrate recognition domain,CRD),但只有C端的CRD区存在Gal家族典型的β-半乳糖苷糖类结合基序。系统进化树表明Bp Gal-8L属于鱼类Gal-8的一种亚型。q PCR结果显示,Bp Gal-8L基因m RNA在各组织中普遍表达,并且在迟缓爱德华氏菌感染后大弹涂鱼肝和脾中表达量呈上调趋势。细菌凝集实验结果显示,只有Bp Gal-8L-C重组蛋白对检测的5种革兰氏阴性菌及2种革兰氏阳性菌有凝集作用,并与乳糖和脂多糖特异性结合。综上所述,Bp Gal-8L与大弹涂鱼抗菌免疫密切相关,且其N端和C端CRD区发挥不同作用。