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Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

陕西省洛南地区二叠系石盒子组砂岩特征及物源区探讨

为了认识陕西省洛南地区二叠系石盒子组砂岩特征及其物源,给探索渭河盆地前新生代基底组成提供依据,本文综合分析了洛南二叠系石盒子组砂岩组分和重矿物特征。结果表明,砂岩物源区主要为再旋回造山带和克拉通地块,物源区与北秦岭造山带的演化关系密切,南秦岭构造带北部下古生界也为石盒子组提供部分物源,中二叠世晚期华北、秦岭板块面接触碰撞,北秦岭构造带快速隆升,南秦岭物源区提供物源减少,砂岩物源主要为中酸性岩浆岩+低级变质岩+沉积岩。二叠纪鄂尔多斯盆地南缘向南延至洛南—栾川断裂附近,洛南地区二叠系石盒子组河流沼泽相含煤岩系沉积应该属于鄂尔多斯盆地南缘沉积,结合渭北—渭河—洛南地区晚古生代地层对比成果,认为渭河地区二叠纪至少曾沉积过二叠系石盒子组地层,渭河新生代盆地基底具有残存二叠系的可能。     

The medium-range variations of the summer monsoon circulation system over east asia

The Asian monsoon circulation system can be divided into two subsystems, i.e., the East Asian monsoon system(EA MS) and the Indian monsoon system (IMS). In this paper the main elements including the Indian monsoon trough, the South Asian high. the upper easterly jet etc. and the interactions between EAMS and IMS arc dealt with. The basic emphasis is put on the medium-range variations of the EAMS. Some significant results arc obtained.     

东北黑土区保护性耕作的发展现状与成效研究

东北黑土区承担着国家粮食安全“稳压器”的重要责任。然而,由于长期超负荷开发利用导致黑土日益退化,黑土资源的永续利用受到严重制约。理论与实践证明,保护性耕作是保护黑土地、推动黑土耕地质量和耕作效益绿色增长的发展模式。综述了保护性耕作的基本内涵及其在东北黑土区的发展现状与技术概况,从保护性耕作在土壤保持、保墒效益、结构改善、固碳培肥和土壤生物多样性增加、节本增效等方面系统评估了东北黑土区实施保护性耕作后的生态与经济效益,提出黑土区实施保护性耕作存在的问题与未来发展方向,以促进黑土地保护与利用协调发展、推动保护性耕作高质量跨越式发展。     

南太行——中条山植物区系地理研究

南太行—中条山地区是中国种子植物区系研究薄弱地区之一。本区约有种子植物1627种,隶属618属142科。通过该区种子植物各类地理成分分析及与相关地区的比较,得出:本地区植物区系具有温带区系特点,又具有温带向亚热带区系过渡性质,是华北植物区系分区南部亚区的一条重要界线。该地区存在许多古老及残遗植物,表明该区系起源古老,同时也是产生新分化的中心之一。     

内蒙古黄旗海不同粒级湖泊沉积物Rb、Sr组成与环境变化

Based on the geochemical elements Rb and Sr in sediments with three different grain size fractions from profile H3 on the northern lacustrine bottomland 13 m above the Huangqihai Lake surface in 1986,the paper investigates the record of palaeolake stand state, sedimentary environmental evolution,and winter monsoon change.First,these samples are separated into three different grain size fractions,i.e.,total sediments,77-20μm and〈20μm. Second,the chemical elements-Rb and Sr-of the grain size separation were tested and analyzed systematically in this paper.Then the elements compositions of these samples are measured using VP-320 mode fluorescence spectrum instrument,respectively.The magnetic susceptibility of these samples is measured using Kappabridge KLY-3 mode instrument made in Czech AGICO Company.The results showed the elements and the ratios varied regularly with the grain size.But the ratio of Rb/Sr in the sediments〈20μm correlates positively with the magnetic susceptibility of these samples.Therefore,the ratio of Rb/Sr in the fraction〈20 μm from the lake sediments reflected the strengthening of the weathering in the deposition sites.It is a good indicator of the summer monsoon-induced weathering and pedogenesis fluctuations and can be used to reconstruct the conditions of the paleoclimate and paleoenvironment.     

大兴安岭北段洛古河地区晚寒武世花岗岩的岩石成因及其对兴蒙造山带构造演化的约束

大兴安岭北段早古生代花岗岩主要分布于漠河—塔河一线。对漠河北部洛古河二长花岗岩开展岩石年代学与岩石地球化学研究。二长花岗岩中锆石为岩浆成因,LA-ICP-MS U-Pb测年表明,其形成时代为晚寒武世（486±3 Ma）。岩石具高硅（SiO2=62.75%~73.32%）、富碱（ALK=8.30%~9.45%）、高铝（Al2O3=13.97%~16.53%）、低镁、低钙和低钛的特点,铝饱和指数A/CNK为0.91~1.10,属偏铝质高钾钙碱性岩石系列。SiO2与Al2O3和P2O5具有较好的负相关性;富集Rb、Th、U、K、Zr、Hf等元素,而强烈亏损Ba、Nb、Ta、Sr、P、Ti等元素,球粒陨石标准化的稀土元素配分曲线图中呈轻稀土元素富集、重稀土元素亏损的右倾型配分模式,显示了Ⅰ型花岗岩特征。综合分析认为,洛古河岩体形成于西伯利亚板块南缘与额尔古纳等微陆块碰撞造山晚期由挤压体制向拉张体制转换的后碰撞构造环境。     

政府规划约束下的城市空间增长多智能体模拟模型

构建政府规划约束下的包含多种类型智能体和环境因素的城市空间增长多智能体模拟模型,通过居民、开发商和政府三类智能体之间以及智能体与环境之间的相互作用模拟了城市空间增长情况。模型将城市建设用地规划区纳入政府的土地利用政策,探讨了政府规划因素对城市建设用地增长的影响,并根据土地集约利用的原则对模拟结果进行了优化。在Repast和ArcGIS的实验环境下,以南京市中心城区(长江以南)为研究区设置了四组土地利用情景,模拟了2001-2007年研究区的城市建设用地增长情况,对比不同情景下模型的模拟精度,说明正确合理的政府规划引导对城市空间增长的重要作用以及对模拟结果进行优化的必要性。该模型有助于揭示城市空间增长机制,为政府宏观调控政策和规划制定提供参考。     

Measuring place-based accessibility under travel time uncertainty

Travel time uncertainty has significant impacts on individual activity-travel scheduling, but at present these impacts have not been considered in most accessibility studies. In this paper, an accessibility evaluation framework is proposed for urban areas with uncertain travel times. A reliable space-time service region (RSTR) model is introduced to represent the space-time service region of a facility under travel time uncertainty. Based on the RSTR model, four reliable place-based accessibility measures are proposed to evaluate accessibility to urban services by incorporating the effects of travel time reliability. To demonstrate the applicability of the proposed framework, a case study using large-scale taxi tracking data is carried out. The results of the case study indicate that the proposed accessibility measures can evaluate large-scale place-based accessibility well in urban areas with uncertain travel times. Conventional place-based accessibility indicators ignoring travel time reliability can significantly overestimate the accessibility to urban services.