Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

Tectonic and magmatic ridges in the eltanin fault system,South Pacific

A Seabeam reconnaissance of the 400 km-long fast-slipping (88 mm yr^-1) Heezen transform fault zone and the 55 km-long spreading center that links it to Tharp transform defined and bathymetrically described several types of ridges built by tectonic uplift and volcanic construction. Most prominent is an asymmetric transverse ridge, at which abyssal hills adjacent to the fault zone have been raised 2–3 km above normal rise-flank depths. Topographic and petrologic evidence suggests that this uplift, which has produced a 5400 m scarp from the crest of the ridge to the floor of a 10 km-wide transform valley, is caused by rapid serpentinization of upper mantle which has been exposed to hydrothermal circulation by fault-zone fracturing of an unusually thin crust. Transverse ridges have been thought atypical of fast-slipping transforms. One class of volcanic ridge more common at these sites is the overshot ridge, formed by prolongation of spreading-center rift zones obliquely across the transform. Overshot ridges are well developed at Heezen transform, especially at the eastern end where an eruptive rift zone extending 60 km from the southern tip of the East Pacific Rise has built a transform-parallel ridge that fills the eastern transform valley. Obliteration of fault-zone structure by ridges overshooting from the spreading center intersections means that the topography of the aseismic fracture zones is not just inherited from that of the active transform fault zone. The latter has several en echelon and overlapping fault traces, linked by short oblique spreading axes that generally form pull-apart basins rather than volcanic ridges. Interpretation of the origin and pattern of the fault zone's tectonic and volcanic relief requires refinement of the plate geography and history of this part of the Pacific-Antarctic boundary, using new Seabeam and magnetic traverses to supplement and adjust the existing geophysical data base.     

Basement imaging with Side-Looking Seismics

The multistreamer Side-Looking Seismic system presented in this paper makes a sonograph of uncovered or buried crustal topography, thus revealing the structural fabric of the oceanic basement, even when this is covered with a sedimentary layer. Major elements of the system are an airgun as a sound source, five single-channel parallel streamers and two minicomputers for signal capture and processing.The system is used simultaneously for enhanced single-channel seismic profiling and for side-looking seismics. A vertical section with an improved signal-to-noise ratio and a suppression of side-echoes is produced on a digital seismic recorder. Primary side-looking seismic output in the form of 5 profiles with different angles of incidence is obtained within 10 seconds. This part of the processing can be done in real time.In sediment-covered areas the low frequencies used cause the slanted profiles (the side beams in the primary output) to be side-looking sonar images of buried topography. The projection process yielding final side-looking output corrects for slant range deformation caused by the water column and, if necessary, for deformation caused by refraction within the sedimentary column. The result approaches a conformal map of the structure of the traversed basement. Swath width is mainly determined by water depth and refraction effects in the sediment. In Madeira abyssal plain a swath width of 8000 m was attained in a water depth of 5000 m.Within the swath, oceanic basement structures are recognized in the form of elongate more or less parallel reflectors. They are interpreted as buried spreading topography. The lack of side-echoes within fracture zones combined with typical wall signatures can be used to trace fracture zones. These features are demonstrated for an area in Madeira abyssal plain.     

Particle transport from the West African shelves of Liberia and Sierra Leone to the deep sea: A chemical approach

The chemical and mineralogical composition of suspended particle suites has been used to assess off-shelf transport on the West African shelves of Liberia and Sierra Leone. Using the ratios of Si/Al, Fe/Al, Mg/Al and Mn/Al as tracers, it was possible to detect shelf-derived materials in slope waters. In the majority of cases, these inputs could not have been detected using particle mass or light scattering measurements and could only be measured by using the chemical signatures of the particulate matter. At the time of sampling, the suspensate which had been moved seaward over the slope was detected adjacent to submarine canyons and highly turbid areas on the outer shelf. The chemical and mineralogical composition of the suspensates, and sediments in the adjacent eastern Atlantic basin are similar to those found in the water column seaward of the West African continental shelves and yet distinct from the Sahelian dust which is considered the major source of sedimentary material for the tropical and semi-tropical areas of the deep eastern basin. These findings suggest that materials from this shelf area could be a more important input to the deep sea than was previously realised.     

Bioavailability: the organism as sensor

The use of a system of continuous culture of phytoplankton at constant population density, the ‘cage-culture’ turbidostat, as a monitor of the bioavailability of pollutant materials, is illustrated. In the case of proposed dredge spoils from two polluted areas, it is shown that bulk analyses for scheduled materials cannot be equated with bioavailability. Monitoring for the effect of the whole pollutant mixture on a properly chosen test population could obviate the necessity for chemical analyses for each component.     

Casualties in dry bulk shipping (1963–1996)

In recent years, bulk carriers have been identified with high risks of catastrophic structural failure and foundering, and with heavy loss of human life. This study, based upon Lloyd's of London casualty records, identified four risk factors that had significant, independent effect upon the likelihood of a bulk carrier foundering. The risk of foundering increased with the age of the ship, and was related to the ship's flag of registration. Most importantly, however, increased risks were found for heavy cargoes of iron ore and scrap steel or iron, and for trading routes to the Far East and from Europe to North America. Additional safety measures, in particular regarding ship design and high-risk trades, may well be necessary to reduce the high casualty rates.     

Spatial variability in petrophysical properties in Upper Maastrichtian chalk outcrops at Stevns Klint, Denmark

The spatial variability of porosity and permeability was determined for a section in the uppermost Maastrichtian chalk exposed in the Sigerslev quarry at Stevns Klint. The aims were threefold: (1) to quantify the spatial variability pattern and its link to geology by applying systematic variogram analysis procedure, (2) to detect potential cyclicity in the petrophysical properties in this pure and overtly homogeneous chalk, and (3) to compare the section with chalk reservoirs in the North Sea in order to asses to what extent this onshore sequence has a potential as an analogue of the offshore, more deeply buried chalk reservoirs. The interval is of comparable stratigraphic age to the uppermost reservoir zones in the Tor Formation of the North Sea chalk reservoirs. The variability and spatial correlation of porosity and permeability in both horizontal and vertical directions are interpreted in a geological context and show indications of small-scale heterogeneity at 15–25 cm scale, but the clear cyclic layering described from other chalk deposits is not recognised at this locality. The investigated outcrop is not a close analogue to North Sea reservoir chalk, but some aspects are common including basic material properties, porosity/permeability trends and the variability pattern. The outcrop has a potential as analogue for some of the onshore subsurface chalk successions at shallow burial depth that form important aquifers.     

The Southern Antarctic Circumpolar Current Front: physical and biological coupling at South Georgia

The coupling of physics and biology was examined along a 160 km long transect running out from the north coast of South Georgia Island and crossing the Southern Antarctic Circumpolar Current Front (SACCF) during late December 2000. Surface and near surface potential TS properties indicated the presence of three water types: a near-shore group of stations characterised by water which became progressively warmer and fresher closer to South Georgia, an offshore grouping in which sea surface temperatures and those at the winter water level were relatively warm (1.8°C and 0.5°C, respectively), and a third in which surface and winter water temperatures were cooler and reflected the presence of the SACCF. The transect bisected the SACCF twice, revealing that it was flowing in opposite directions, north-westward closest to South Georgia and south-eastwards at its furthest point from the island. The innermost limb was a narrow intense feature located just off the shelf break in 2000–3500 m of water and in which rapid surface baroclinic velocities (up to 35 cm s⁻¹) were encountered. Offshore in the outermost limb, shown subsequently to be a mesoscale eddy that had meandered south from the retroflected limb of the SACCF, flow was broader and slower with peak velocities around 20 cm s⁻¹. Chlorophyll a biomass was generally low (<1 mg m⁻³) over much of the transect but increased dramatically in the region of the innermost limb of the SACCF, where a deepening of the surface mixed layer was coincident with a subsurface chlorophyll maximum (7.4 mg m⁻³) and elevated concentrations down to 100 m. The bloom was coincident with depleted nutrient concentrations, particularly silicate, nitrate and phosphate, and although ammonium concentrations were locally depleted the bloom lay within an elevated band (up to 1.5 mmol m⁻³) associated with the frontal jet. Increased zooplankton abundance, higher copepod body carbon mass and egg production rates all showed a strong spatial integrity with the front. The population structure of the copepods Calanoides acutus and Rhincalanus gigas at stations within the front suggested that rather than simply resulting from entrainment and concentration within the jet, increased copepod abundance was the result of development in situ. Estimates of bloom duration, based on silicate and carbon budget calculations, set the likely duration between 82 and 122 d, a figure supported by the development schedule of the two copepod species. Given this timescale, model outputs from FRAM and OCCAM indicated that particles that occurred on the north side of South Georgia in December would have been in the central-southern Scotia Sea 2–3 months earlier, probably in sea ice affected regions.