长江口盐度梯度下不同形态碳的分布、来源与混合行为

河口碳的生物地球化学过程是全球碳循环的重要组成。通过测定溶解无机碳（DIC）及其稳定同位素丰度（δ13C_DIC）,溶解有机碳（DOC）,有色溶解有机物（CDOM）,颗粒有机碳（POC）及其稳定同位素丰度（δ13C_POC）与元素比值（N/C）及相关指标,研究了2014年7月长江口盐度梯度下不同形态碳的分布、来源和混合行为。结果表明,DIC浓度、DOC浓度、POC含量分别为1 583.2~1 739.6 μmol/L,128.4~369.4 μmol/L和51.2~530.8 μmol/L,这些不同形态碳及CDOM的荧光组分的分布模式相似,均是从口内到口外,整体呈现先增大后减小的趋势,并与盐度呈现非保守混合行为。添加作用主要发生在在口门处最大浑浊带附近。与含量相反,从口内到口外,δ13C_DIC和δ13C_POC均呈现逐渐减小再增大的趋势,在口门附近达到最低值,分别为-9.7‰和-26.7‰。在口门附近不同形态碳含量上升及δ13C_DIC、δ13C_POC的降低可能主要与沉积物再悬浮及微生物作用有关。基于蒙特卡洛模拟的三端元混合模型的结果显示,河口内外POC来源变化明显,口内POC以陆源有机碳贡献为主,平均为62.3%,口外海源贡献逐渐增加。CDOM相关参数结果表明长江口CDOM主要来自陆源输入,海源及人类活动等也对其产生影响。     

基于线粒体COI和16S rRNA基因的中国沿海相手蟹系统发育研究

相手蟹科的诸多种类因其形态极其相似成为方蟹总科分类中疑问较多的一个类群。通过对中国沿海相手蟹线粒体COI和16S rRNA基因序列进行分子系统发育分析,结果表明14种相手蟹COI和16S rRNA基因序列之间差异分别为5.7%～14.5%和1.5%～12.1%,均达到了种间差异水平。构建的系统发育树显示,14种相手蟹分别为独立有效物种,但分属于拟相手蟹属和近相手蟹属的4种拟相手蟹和3种近相手蟹,没有分别形成2个独立的支系,而是混合聚成一大支系。而属于螳臂相手蟹属的无齿螳臂相手蟹则首先与属于中相手蟹属的中华中相手蟹聚成一支,再与红螯螳臂相手蟹聚为一大支,表现出与形态分类的不一致。错综复杂的分子系统关系预示着相手蟹类为多系起源,也表明它们之间的种间关系乃至于属间关系尚有诸多问题有待进一步厘定。     

胶州湾沉积物中石油降解菌的降解能力及多样性研究

2013年青岛输油管道爆炸,大量石油污染了附近海岸。课题组采集了污染的沉积物样品,以原油为唯一碳源和能源,富集了四个石油降解菌群。生物多样性和群落分析表明,Luteibacter、Parvibaculum 和属于食烷菌科的一个属是降解菌群的主要优势菌,都属于变形菌门。从石油降解菌群中分离筛选,获得了9株具有不同16S rRNA基因序列的降解菌,分别属于8个属。重量法测定降解菌的石油降解率,其中5株的石油降解率大于30%。GC-MS分析结果表明,石油降解菌多倾向于降解烷烃,对多环芳烃的降解能力较差,其中5株细菌的烷烃降解率较大,仅1株菌D2对多环芳烃的降解率较大,其降解率在34.9%到77.5%。通过对高通量数据的分析,表明食烷菌属是菌群A和菌群E的主要降解菌群,其中筛选获得的菌株E4可能是菌群E的一株优势降解菌。本研究所筛选菌株证明了其石油降解潜力,为油污染海滩生物修复提供了菌株资源。     

Vortex-Induced Vibrations of A Long Flexible Cylinder in Linear and Exponential Shear Flows

A numerical study based on a wake oscillator model was conducted to determine the response performance of vortex-induced vibration(VIV) on a long flexible cylinder with pinned-pinned boundary conditions subjected to linear and exponential shear flows. The coupling equations of a structural vibration model and wake oscillator model were solved using a standard central finite difference method of the second order. The VIV response characteristics including the structural displacement, structural frequency, structural wavenumber, standing wave behavior,travelling wave behavior, structural velocity, lift force coefficient and transferred energy from the fluid to the structure with different flow profiles were compared. The numerical results show that the VIV displacement is a combination of standing waves and travelling waves. For linear shear flow, standing waves and travelling waves dominate the VIV response within the low-velocity and high-velocity zones, respectively. The negative values of the transferred energy only occur within the low-velocity zone. However, for exponential shear flow, travelling waves dominate the VIV response and the negative energy occurs along the entire length of the cylinder.     

Examination of Extraordinary Transmission of Waves Propagation through Gaps of Vertical Thin Barriers in Channels by A Hypersingular Boundary Element Method

The extraordinary transmission(ET) phenomenon is examined for waves propagating through gaps of vertical thin barriers in channels with a hypersingular boundary element method model on the linear potential theory, and an estimate formula based on small gap approximation for predicting the number of ET frequencies is proposed.Numerical computations are carried out to examine the influences of barrier number, barrier interval, gap size, gap position and barrier arrangement on extraordinary transmission and wave height in the channel. It shows that all of those factors evidently affect the extraordinary transmission frequencies. The contours of wave amplitude show that very high waves can be excited in the basins between barriers at the extraordinary transmission frequencies. Proper arrangement of barriers in a channel can avoid the occurrence of ET phenomenon and reduce wave height in the channel.     

Numerical Simulation on Flow Past Two Side-by-Side Inclined Circular Cylinders at Low Reynolds Number

A series of three-dimensional numerical simulations is carried out to investigate the effect of inclined angle on flow behavior behind two side-by-side inclined cylinders at low Reynolds number Re=100 and small spacing ratio T/D=1.5 (T is the center-to-center distance between two side-by-side cylinders, D is the diameter of cylinder). The instantaneous and time-averaged flow fields, force coefficients and Strouhal numbers are analyzed. Special attention is focused on the axial flow characteristics with variation of the inclined angle. The results show that the inclined angle has a significant effect on the gap flow behaviors behind two inclined cylinders. The vortex shedding behind two cylinders is suppressed with the increase of the inclined angle as well as the flip-flop gap flow. Moreover, the mean drag coefficient, root-mean-square lift coefficient and Strouhal numbers decrease monotonously with the increase of the inclined angle, which follows the independent principle at small inclined angles.     

紫刺参早期生长发育与体色形成的研究

本研究对紫刺参胚胎和幼体发育过程进行了显微观测,比较分析了其幼体早期生长情况与体色形成过程。结果表明:(1)在水温21.0±0.2℃条件下,紫刺参受精卵在受精10～15min后释放第一极体,5h 30min～6h 30min进入囊胚期, 18～20h发育成原肠胚; 30～34h进入耳状幼体阶段, 8～10d变态发育为樽形幼体, 12～14d发育成稚参;紫刺参胚胎和幼体发育时序与普通刺参无显著差异。(2)紫刺参早期发育中的樽形幼体发生率和附着变态率分别为58.4±4.7%和45.1±2.7%,高于普通刺参。(3)紫刺参浮游阶段后期随日龄增长表现出较普通刺参发育更快的优势,在日龄6～7d表现出显著差异(P0.05)。(4)紫刺参稚参在日龄35d前后自背部开始着色,50d～60d腹部开始着色,110d体表紫色转变近乎成参体色,完成变色过程。     

虚拟地理环境中基于多模式的多人动作捕捉方法

虚拟地理环境对人类理解、探索地理现象以及发现地理现象之间的联系起到很重要的作用。在基于VR技术的虚拟地理环境中更高效,更自然的交互对虚拟地理环境系统的可用性以及理解、探索地理现象的效率至关重要。当前,人机交互中更自然便捷的交互依赖于动作捕捉技术,而现有的基于单种捕捉模式的动作捕捉方式,易受到遮挡、定位漂移等的影响,捕捉精度受到限制,并只能捕捉单个人的动作,不能满足虚拟地理环境对动作捕捉技术提出的特殊需求。针对单种模式的动作捕捉设备应用于虚拟地理环境受到的限制,本文分析了虚拟地理环境系统中的交互需求以及其对动作捕捉技术提出的特殊需求,创新性的提出了一种基于多种动作捕捉模式以捕捉多人动作的方法。本文方法集中于如何融合基于多种模式的多台设备的动作数据,描述了多人动作捕捉框架及其涉及到的技术,在动作捕捉效果上具有明显提升,能够捕捉多人动作,并开发了原型系统对此方法进行了验证。     

北京市居民地铁出行出发时间弹性时空分布特征研究

伴随城市转型进程的加快,交通需求不断膨胀,导致大城市交通拥堵日趋严重,以调节出行者的选择行为为核心要素的交通需求管理理念成为相关政策的重要理论基础,但现有研究也表明,交通需求管理对出行弹性较高的出行具有显著调节作用,而对出行弹性较低的出行调节作用并不明显。因此,加强出行弹性等居民出行行为研究日益迫切,而公交刷卡数据等新的时空数据为居民复杂出行行为的挖掘提供了新的契机。本文利用北京市2014年3月地铁刷卡数据,以出行者出发时刻的可变性来测度出发时间选择的可改变程度,对居民地铁出行出发时间选择弹性进行测度,并结合GIS空间分析技术对其时空分布特征进行分析。研究表明：① 北京市地铁出行的居民出行弹性平均值为0.521,出发时间选择弹性整体上较大,表明北京居民出发时间选择相对较为灵活;② 北京市居民地铁出行弹性存在时空差异,居民个体休息日出行弹性高于工作日,一天中高峰时段出行弹性高于非高峰时段;③ 居民出行弹性存在空间自相关,倾向于在空间上发生集聚,存在明显的冷热点区域;内城居民的出行弹性明显高于城市外围居民。     

Growth and feeding habits of invasive Pseudorasbora parva in the Chabalang Wetland(Lhasa,China) and its trophic impacts on native fish

In recent years, fish invasion has become one of the main reasons for the decline of nativefish stocks. Pseudorasbora parva is considered one of the major invasive species worldwide. The present study investigated the fish resources of the Chabalang Wetland(Lhasa, Tibet) during dif ferent seasons in2009 and 2013. Four hundred and twelve individuals were subsampled to estimate age, growth, and feeding habit of P. parva. Furthermore, food relationships between P. parva and the native Schizothoracinae fish were also examined. The results revealed a significant shift in species composition and community structure characterized by the disappearance of native fish and outbreak of non-native fish. The percentage of nonnative P. parva in the fish collections significantly increased from 33.64% in 2009 to 64.08% in 2013.The standard length(SL) ranged from 22.00 to 78.71 mm, and their age was 1–5 a. The von Bertalanf fy function was used to model the observed length-at-age data as Lt =112.19(1– e~(-0.1495(t +0.8012))) for females and as Lt =123.12(1– e~(-0.1500(t +0.7132))) for males. The results indicated that P. parva in Tibet has lower growth and mortality rates compared with that from the native ranges. Ninety-seven prey taxa belonging to 9 prey categories were identified in the gut of 38 P. parva. P. parva can be considered a generalized and opportunistic predator, competing with the native fish, especially Schizothorax o'connori, Schizopygopsis younghusbandi younghusbandi, and Ptychobarbus dipogon, for Bacillariophyta and Chironomid larvae.This is an important reason for the decline in native fish population.