Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

Effects of crab–halophytic plant interactions on creek growth in a S.W. Atlantic salt marsh: A Cellular Automata model

The Bahía Blanca Estuary (38° 50′ S, and 62° 30′ W) presents salt marshes where interactions between the local flora (Sarcocornia perennis) and fauna (Chasmagnathus granulatus) generate some kind of salt pans that alter the normal water circulation and condition its flow and course towards tidal creeks. The crab–vegetation dynamics in the salt marsh presents variations that cannot be quantified in a reasonable period of time. The interaction between S. perennis plant and C. granulatus crab is based on simple laws, but its result is a complex biological mechanism that causes an erosive process on the salt marsh and favors the formation of tidal creeks. To study it, a Cellular Automata model is proposed, based on the laws deduced from the observation of these phenomena in the field, and then verified with measurable data within macroscale time units. Therefore, the objective of this article is to model how the interaction between C. granulatus and S. perennis modifies the landscape of the salt marsh and influences the path of tidal creeks. The model copies the basic laws that rule the problem based on purely biological factors.The Cellular Automata model proved capable of reproducing the effects of the interaction between plants and crabs in the salt marsh. A study of the water drainage of the basins showed that this interaction does indeed modify the development of tidal creeks. Model dynamics would likewise follow different laws, which would provide a different formula for the probability of patch dilation. The patch shape can be obtained changing the pattern that dilates.     

The mysid Siriella armata as a test organism in toxicology: Effects of cadmium

A shrimp-like crustacean of the coastal Zooplankton in the north-western Mediterranean Sea, the mysid Siriella armata, offers a test organism whose life cycle is entirely controllable under laboratory conditions. The effects of the heavy metal cadmium were studied on adults and juveniles cultured in the laboratory including acute toxicity, bioaccumulation and effects on growth, ingestion and moult cycle.     

Hydrogeochemistry and isotope analyses used to determine groundwater recharge and flow in low-gradient catchments of the province of Buenos Aires,Argentina

The region known as Pampa Plain, in Argentina, is a vast area characterized by slopes of less than 0.05%. The surface sediment is silty sand, mainly Aeolian, referred to as Pampean Loess, which is a phreatic aquifer unit of utmost importance for the water supply of the region. On account of the slight gradient, hydrogeological analyses using only hydraulic measurements are difficult to perform, often leading to confusing results. Thus, the study presented relies on hydrochemical modeling and isotopic determinations as well. The study area comprises three catchments in the inter-mountainous area corresponding to El Moro, Tamangueyú and Seco creeks, and covering 2,570 km² in the province of Buenos Aires. Measurements of piezometric levels and samples of groundwater, surface water and rainwater were carried out between January and March 2005. Major ion results were analyzed by means of hydrochemical graphs and hydrogeochemical modelling using NETPATH. The resulting data show that it is possible to identify local changes in recharge, flow direction and stream/groundwater relationship by using hydrochemical and isotopic information, which may become a useful and more precise tool for the study of particularly flat landscapes.     

Low frequency flickering of TT arietis: Hard and soft X-ray emission region

Using archival ASCA observations of TT Arietis, X-ray energy spectra and power spectra of the intensity time series are presented for the first time. The energy spectra are well-fitted by a two continuum plasma emission model with temperatures 1 keV and 10 keV. A coherent feature at 0.643 mHz appeared in the power spectra during the observation.     

The contact binary system YY Eri

Relatively new and unanalysed photometric data-sets of the contact binary system YY Eri are presented. The light curves have been analysed using information limit optimization techniques, and the Binary Maker program of Bradstreet (1992).Comparison of the results allows insight into determinacy questions affecting the W UMa type of light curve, which relates to our general understanding of contact binaries. A limiting contact configuration cannot be ruled out on the basis of empirical data-analysis alone.Very careful analysis of the photospheric flux distribution over the surface of the Sun may help establish appropriate values of the gravity brightening parameter for cool dwarf stars. At present, however, empirical photometric information on contact binaries is non-discriminatory: i.e. alternative cosmogonies can find alternative support from available evidence. The implication is then for more and better observational data to allow better real independent parameter determination.     

Solar models with strong turbulence

A series of evolutionary models for the Sun were constructed using the strong turbulence model of the variable mixing-length theory (AMLT) of Canuto (1990). The present values of the solar model were obtained by an initial compositionX = 0.754,Z = 0.019, and(x) = 1.1. The physical variables which were obtained by applying the strong turbulence case of AMLT were similar to those of weak turbulence model (Kzlolu and Civelek, 1992) if we focused on the fitting of present solar radius and luminosity.     

Structure of the expanding atmosphere of P Cygni

With the aid of the spectra taken in the years 1959–1968, a physical analysis of the atmosphere of P Cygni has been carried out and the motions of the atmosphere have been studied. The variations of radial velocities, the velocity progressions of Balmer and Hei lines, the high rate of mass loss (2×10^–5 M yr^–1), the features of the observed line profiles, especially that of H-K lines of Caii andD ₁-D ₂ lines of Nai confirm the conclusion of Van Blerkom (1978), concerning the assumption of an accelerating atmosphere for P Cygni. The electron density variation with the radius seems to ben _e r ^–5/2, with an average value of 7×10¹¹cm^–3 at the lower boundary of the atmosphere.In order to explain the two absorption components of observed lines, an atmospheric model based on the assumption of three envelopes, two of which accelerate gradually with two different velocity laws (up to 11.2r _c ), and the third of which accelerates rapidly with a standard velocity law (beyond 11.2r _c ) has been developed. From this model and the observed profiles, the geometrical thicknesses of the line-forming regions of H, H, H, and H are derived.The observations were obtained at Haute Provence Observatory (CNRS).     

Analysis of hydrologic relations between Eğirdir-Beyşehir-Suğla lakes system and adjacent basins by means of remote sensing techniques (southern Turkey)

The study area is situated within the complex structure and karst system of the western Taurids. Basinwide interpretation of the structural features, each of which has great importance, will enlighten many complicated hydrogeologic problems encountered in the area. Thus, considering the previous views on the structural geology of the area, an interpretation of the structural and tectonic features of the study area by means of satellite images was undertaken, and based on the data gained, new approaches were suggested to solve the hydrogeological problems. In particular, determination of the recharge-discharge mechanisms of the Olukköprü and Dumanli karst springs, which are the most important karst groundwater discharge points in the region, has been attempted. Within the framework of this study, a tectonic-lineament map of a large area covering Eirdir, Beyehir, and Sula lakes at the north and the basins to the south of these lakes was prepared.     

Preliminary results from the spectra of V471 tauri

The findings about this unusual eclipsing variable which possesses a white-dwarf component are reported. It was found that the H and K absorption lines of Caii are strongly pressure-broadened, and exhibit two double-reversal emission components. The radial velocities derived from the central strong emission components of Caii (H and K) are in agreement with the mean radial velocity curve obtained from the metallic absorption lines of the existing elements, whereas H (4340.47 Å) does not conform with the mean radial velocity curve. In certain orbital phases the H (6562.82 Å) line occurs in emission.