Generation of reactive oxygen species in different fractions of the coelomocytes of holothurian Eupentacta fraudatrix in response to the thermostable toxin of Yersinia pseudotuberculosis in vitro

Pure fraction (92%–95%) of phagocytes (FP) and a mixture of amoebocytes (62%) and morula cells (38%)-FPMC- of the holothurianEupentacta fraudatrix' (Holothuroidea, Dendrochirota) were obtained by using ficoll-verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin ofYersinia pseudotuberculosis (TST) at different concentrations (0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration-dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5-fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. This work was financially supported by the Russian Foundation for Fundamental Research Grant (No. 00-04-48949).     

Electric Discharges in the Protoplanetary Nebula as a Source of Impulse Magnetic Fields to Promote Dust Aggregation

Lightning discharge generated in the protoplanetary nebula is viewed as a temporally isolated surge in the flow of electrically charged particles, similar to that of terrestrial lightning. If the current is intense enough, a powerful circular impulse magnetic field is generated around the instantaneous virtual electric conductor. Such magnetic field is capable of magnetizing dust grains containing ferromagnetic components present in its vicinity to their saturation levels. As a result, dust grains attract one another, forming the aggregates. This magnetically driven attraction suggests an important process possibly operational at an early stage of the planetary accretion. Based on both a classical model for electric conductor, and the theory of Lienard–Wiechert electromagnetic potentials, our calculations show that the magnetic impulse due to a discharge channel of a few cm in diameter transferring a charge of about 10⁴ electrons reaches as high as 10 T. At these magnetic fields, the ferromagnetic dust grains, and possibly the already-formed larger aggregates as well, are easily magnetized to the saturation levels, producing compact clusters exhibiting permanent magnetic moments.     

The Scattered Disk Population and the Oort Cloud

The trans-Neptunian belt has been subject to a strong depletion that has reduced its primordial population by a factor of one hundred over the solar system's age. One by-product of such a depletion process is the existence of a scattered disk population in transit from the belt to other places, such as the Jupiter zone, the Oort cloud or interstellar space. We have integrated the orbits of the scattered disk objects (SDOs) so far discovered by 2500 Myr to study their dynamical time scales and the probability of falling in each of the end states mentioned above, paying special attention to their contribution to the Oort cloud. We found that their dynamical half-time is close to 2.5 Gyr and that about one third of the SDOs end up in the Oort cloud.     

F stars, metallicity and the ages of red galaxies at z > 1

We explore whether the rest-frame near-ultraviolet spectral region, observable in high-redshift galaxies via optical spectroscopy, contains sufficient information to allow the degeneracy between age and metallicity to be lifted. We do this by first testing the ability of evolutionary synthesis models to reclaim the correct metallicity when fitted to the near-ultraviolet spectra of F stars of known (subsolar and supersolar) metallicity. F stars are of particular interest because the rest-frame near-ultraviolet spectra of the oldest known elliptical galaxies at   z > 1  appear to be dominated by F stars near to the main-sequence turn-off.
We find that, in the case of the F stars, where the Hubble Space Telescope ultraviolet spectra have a high signal-to-noise ratio, fitting models in which the metallicity is allowed to vary as a free parameter is rather successful at deriving the correct metallicity. As a result, the estimated turn-off ages of these stars yielded by model-fitting are well constrained. Encouraged by this we have fitted these same variable-metallicity models to the deep, optical spectra of the   z ≃ 1.5 mJy  radio galaxies 53W091 and 53W069 obtained with the Keck telescope. While the age and metallicity are not so easily constrained for these galaxies, we find that even when metallicity is allowed as a free parameter, the best estimates of their ages are still ≥3 Gyr, with ages younger than 2 Gyr now strongly excluded. Furthermore, we find that a search of the entire parameter space of metallicity and star formation history using MOPED leads to the same conclusion. Our results therefore continue to argue strongly against an Einstein–de Sitter universe, and favour a Λ-dominated universe in which star formation in at least these particular elliptical galaxies was completed somewhere in the redshift range   z = 3–5  .     

Potential of the VLTI for linking stellar frames to ICRF

The question of positioning the optical counterparts of the ICRF quasars is outlined in the perspective of future space astrometry missions, which ultimately will bring a new realization of the ICRS in the optical range. Ground-based interferometry with a dual-field observing mode (PRIMA/VLTI),together with the missions DIVA and FAME, will have a key role in building an extragalactic reference frame in the optical/near-IR range with about the same accuracy as that of the present (VLBI) primary frame. This revised version was published online in July 2006 with corrections to the Cover Date.     

An Instantaneous-Frequency Filtering Technique for High-Frequency Radar Oceanography

High-frequency (HF) radar systems are remote sensing tools that can be used to measure oceanographic parameters. Problems can occur when using the conventional periodogram (PG) method for computing power spectral estimates from backscattered radar signals. Temporal and spatial inhomogeneities within the radar measurement region can cause distortion in the spectra. This paper describes an instantaneous-frequency (IF) filtering technique that has been developed to measure the first-order modulation contained within the radar signal. Successful removal of this modulation is shown to yield an increased quality and quantity of ocean measurements     

Survival of organic phases in porous IDPs during atmospheric entry: A pulse‐heating study

Abstract— In this study, we have performed pulse‐heating experiments at different temperatures for three organic molecules (a polycyclic aromatic hydrocarbon [PAH], a ketone, and an amino acid) absorbed into microporous aluminum oxide (Al₂O₃) in order to imitate the heating of the organic molecules in interplanetary dust particles (IDPs) and micrometeorites (MMs) during atmospheric entry and to investigate their survival. We have shown that modest amounts (a few percent) of these organic molecules survive pulse‐heating at temperatures in the 700 to 900 °C range. This suggests that the porosity in IDPs and MMs, combined with a sublimable phase (organic material, water), produces an ablative cooling effect, which permits the survival of organic molecules that would otherwise be lost either by thermal degradation or evaporation during atmospheric entry.