Screen and effect analysis of immunostimulants for sea cucumber,Apostichopus japonicus

Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal's coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day 1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection (P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.     

西宁地区次生盐渍土的分布特征及地基处理方法

西宁地区次生盐渍土主要分布于山麓斜坡带高阶地和河谷高漫滩,前者属中～弱硫酸、亚硫酸盐渍土,且多具中等～强烈湿陷性,盐份主要来自斜坡后缘含盐碎屑岩风化物及易溶盐;后者为中等亚氯、亚硫酸盐渍土,溶于土中的盐份系浅层咸地下水常年毛细上升蒸发所致。其次生盐渍土的松胀性、溶陷性、腐蚀性较强,工程建设要引起重视。     

集合滤波和三维变分混合数据同化方法研究

发展了一种新的混合数据同化方法——基于集合滤波和三维变分的混合数据同化方法。该方法将集合调整卡尔曼滤波(ensemble adjustment Kalman filter,EAKF)得到的集合样本扰动通过一个转换矩阵的形式直接作用到背景场上,利用顺序滤波的思想得到分析场的一个扰动;然后在三维变分(three dimensional variational analysis,3D-Var)的框架下与观测数据进行拟合,从而给出分析场的最优估计。文中以Lorenz63模型为例,开展了理想数据同化试验,结果表明,相比于集合调整卡尔曼滤波,这种新的混合同化方法可以给出更好的同化结果。