Length-specific carbon content of theDaphnia population in a large subalpine lake, Lago Maggiore (Northern Italy): The importance of seasonality

We compared three methods for calculating the standing stock biomass ofDaphnia hyalina galeata population in Lago Maggiore during a single year. We applied body length/carbon regression equations (LCR) obtained under controlled laboratory conditions and directly from field data on carbon and body length to field data on length-specific abundance. Laboratory-derived equations satisfactorily described our field estimates of length-specific carbon content in spring. As the length-standardized carbon content decreased exponentially along the season, we included time of the year as an auxiliary variable, and tested whether it affected slope, intercept or both. The decrease had no effect on slope but reduced the intercept of the LCR. We also calculated biomass, using a single mean value from the literature. By comparing the results with those obtained by simple conversion of individuals number into carbon we might discriminate between the effect of changes in the size frequency distribution of the population and the decrease with time of the length-standardized carbon content. Although we tested the different models for non-ovigerous females, the results did not change when we included also data on females with eggs in the first three developmental stages, whereas they changed substantially when we included females with embryos. We formulate two hypotheses to explain this result and discuss possible implications for the calculation of the standing stock biomass ofDaphnia population.     

High‐temperature gold‐copper extraction with chloride flux in lava tubes of Tolbachik volcano (Kamchatka)

Subvolcanic environments in supra‐subduction zones are renowned for hosting epithermal deposits that often contain electrum and native gold, including bonanza examples. This study examined mineral assemblages and processes occurring in shallow‐crust volcanic settings using recent eruption (2012–2013) of the basaltic Tolbachik volcano in the Kamchatka arc. The Tolbachik eruptive system is characterized by an extensive system of lava tubes. After cessation of magma input, the tubes maintained the flow of hot oxidized gases that episodically interacted with the lava surfaces and sulphate‐chloride precipitates from volcanic gases on these surfaces. The gas‐rock interaction had strong pyrometamorphic effects that resulted in the formation of molten salt, oxidized (tenorite, hematite, Cu‐rich magnesioferrite) and skarn‐like silicate mineral assemblages. By analogy with experimental studies, we propose that a combination of these processes was responsible for extraction of metals from the basaltic wall rocks and deposition of Cu‐, Fe‐ and Cu‐Fe‐oxides and native gold.     

Response of suspended sediment particle size distributions to changes in water chemistry at an Andean mountain stream confluence receiving arsenic rich acid drainage

Acid drainage is an important water quality issue in Andean watersheds, affecting the sustainability of urban, agricultural, and industrial activities. Mixing zones receiving acid drainage are critical sites where changes in pH and chemical environment promote the formation and dissolution of iron and aluminum oxy/hydroxides. These particles can significantly change the speciation of toxic metals and metalloids throughout drainage networks via sorption, desorption, and settling processes. However, little is known about the behavior of particle size distributions (PSDs) in streams affected by acid drainage and their relationship to metal speciation. This work studied: (a) the PSDs for a wide range of mixing ratios found at a fluvial confluence affected by acid drainage, and (b) the response of PSDs and arsenic speciation to environmental changes found when the particles approach complete mixing conditions. The confluence between the Azufre River (pH ~ 2, high concentration of dissolved metals) and Caracarani River (pH = 8.6, low concentration of dissolved metals) was used as a representative model for study. Field measurements show a bimodal PSD with modal diameters of ~50 and 300 μm. At shorter distances from the junction, the smaller modes with smaller particle volumes were dominant across the stream cross‐sections. A systematic shift towards larger particle sizes and larger particle volumes occurred downstream. The analysis of laboratory PSDs for Azufre/Caracarani mixing ratios between 0.01 and 0.5 (pH from 6.2 to 2.3) showed a bimodal trend with ~15 and 50 μm characteristic diameters; larger particles formed at pH>4. When particle suspensions were transferred in laboratory experiments from very low pH to full mixing conditions (pH ~ 2.8 and mixing ratio ~ 0.25) particle sizes varied, and the dissolved arsenic concentration decreased. The observed reaction kinetics were slow compared to the time scale of advective transport, creating opportunities for engineered controls for arsenic. This work contributes to a better understanding of the chemical‐hydrodynamic interactions in watersheds affected by mining, and identifying opportunities to improve water quality at points of use.     

Crustacean decapod assemblages associated with fragmented Posidonia oceanica meadows in the Alboran Sea (Western Mediterranean Sea): composition,temporal dynamics and influence of meadow structure

The decapod assemblage associated with a Posidonia oceanica meadow located near its western limit of biogeographic distribution was studied over an annual cycle. Fauna samples were taken seasonally over a year (five replicates per season) in two sites located 7 km apart, using a non‐destructive sampling method (airlift sampler) for the seagrass. The dominant species of the assemblage, Pisidia longimana, Pilumnus hirtellus and Athanas nitescens, were associated with the protective rhizome stratum, which is mainly used as a nursery. The correlations between decapod assemblage structure and some phenological parameters of the seagrass shoots and wave height were negative or null, which reflects that species associated with the rhizome had a higher importance than those associated with the leaf stratum. The abundance and composition of the decapod assemblage as well as the ecological indexes displayed a seasonality trend with maximum values in summer‐autumn and minimum in winter‐spring, which were related to the seawater temperature and the recruitment periods of the dominant species. The spatial differences found in the structure and dynamics of the assemblages may be due to variations in the recruitment of the dominant species, probably as a result of the influence of local factors (e.g. temperature, currents) and the high dispersal ability of decapods, together with the patchy configuration and the surrounding habitats. The studied meadows are fragmented and are integrated within a mosaic of habitats (Cymodocea nodosa patches, algal meadows, rocky and sandy bottoms), which promotes the movement of individuals and species among them, maintaining a high species richness and evenness.     

Petrogenesis of low-δ<Superscript>18</Superscript>O quartz porphyry dykes,Koegel Fontein complex,South Africa

This paper investigates the origin of low-δ¹⁸O quartz porphyry dykes associated with the 144–133 Ma Koegel Fontein Igneous Complex, which was intruded during the initial phase of breakup of Africa and South America. The 25-km diameter Rietpoort Granite is the largest and youngest phase of activity, and is roofed by a 10-km diameter pendant of gneiss. Quartz porphyry (QP) dykes, up to 15 m in width, strike NW–SE across the complex. The QP dykes that intruded outside the granite have similar quartz phenocryst δ¹⁸O values (average 8.0‰, ± 0.7, n?=?33) to the granite (average 8.3?±?1.0, n?=?7). The QP dykes that intruded the roof pendant have quartz phenocrysts with more variable δ¹⁸O values (average 1.6‰, ± 2.1, n?=?55). In some cases quartz phenocrysts have δ¹⁸O values as low as ? 2.5‰. The variation in δ¹⁸O value within the quartz crystal population of individual dykes is small relative to the overall range, and core and rim material from individual quartz phenocrysts in three samples are identical within error. There is no evidence that quartz phenocryst δ¹⁸O values have been affected by fluid–rock interaction. Based on a ?_quartz?magma value of 0.6‰, magma δ¹⁸O values must have been as low as ? 3.1‰. Samples collected along the length of the two main QP dykes that traverse the roof pendant have quartz phenocryst δ¹⁸O values that range from +?1.1 to +?4.6‰, and ? 2.3 to +?5.6‰, respectively. These δ¹⁸O values correlate negatively (r = ? 0.96) with initial ⁸⁷Sr/⁸⁶Sr, which can be explained by the event that lowered δ¹⁸O values of the source being older than the dykes. We suggest that the QP dykes were fed by magma produced by partial melting of gneiss, which had been variably altered at high temperature by ¹⁸O-depleted meteoric water during global glaciation at ~?550 Ma. The early melts had variable δ¹⁸O value but as melt pockets interconnected during melting, the δ¹⁸O values approached that of average gneiss. Variable quartz phenocryst δ¹⁸O values in the same dyke can be explained by vertical emplacement, at variable rates of ascent along the dyke. The lateral variation in quartz, and hence magma δ¹⁸O value at a particular point along a single dyke would depend on the rate of ascent of magma at that point along the dyke, and the ‘age’ of the particular magma batch.     

New observations on high‐pressure phases in a shock melt vein in the Villalbeto de la Peña meteorite: Insights into the shock behavior of diopside

The petrology and mineralogy of shock melt veins in the L6 ordinary chondrite host of Villalbeto de la Peña, a highly shocked, L chondrite polymict breccia, have been investigated in detail using scanning electron microscopy, transmission electron microscopy, Raman spectroscopy, and electron probe microanalysis. Entrained olivine, enstatite, diopside, and plagioclase are transformed into ringwoodite, low‐Ca majorite, high‐Ca majorite, and an assemblage of jadeite‐lingunite, respectively, in several shock melt veins and pockets. We have focused on the shock behavior of diopside in a particularly large shock melt vein (10 mm long and up to 4 mm wide) in order to provide additional insights into its high‐pressure polymorphic phase transformation mechanisms. We report the first evidence of diopside undergoing shock‐induced melting, and the occurrence of natural Ca‐majorite formed by solid‐state transformation from diopside. Magnesiowüstite has also been found as veins injected into diopside in the form of nanocrystalline grains that crystallized from a melt and also occurs interstitially between majorite‐pyrope grains in the melt‐vein matrix. In addition, we have observed compositional zoning in majorite‐pyrope grains in the matrix of the shock‐melt vein, which has not been described previously in any shocked meteorite. Collectively, all these different lines of evidence are suggestive of a major shock event with high cooling rates. The minimum peak shock conditions are difficult to constrain, because of the uncertainties in applying experimentally determined high‐pressure phase equilibria to complex natural systems. However, our results suggest that conditions between 16 and 28 GPa and 2000–2200 °C were reached.     

Influence of nutritional stress on digestive enzyme activities in juveniles of two marine clam species, Ruditapes decussatus and Venerupis pullastra

The potential use of digestive activities as indicators of the nutritional status in bivalves is discussed in relation to the results obtained in two clam species exposed to starvation and refeeding. Activities of some digestive enzymes (amylase, laminarinase, cellulase, and protease) were measured in juveniles of two commercially interesting species of clams, Ruditapes decussatus and Venerupis pullastra. The specimens were fed normally, being after subjected to a 15-days starvation and a further refeeding period. Samples were obtained at different moments of such feeding schedule to evaluate enzymes as well as weight (live, dry and organic) and length, in order to calculate growth rates and feeding efficiencies. Starvation led to a major decrease in clam growth as measured by dry weight and a negative growth as measured by organic weight, this coinciding with a certain degree of growth of the shell and a consumption of soft tissue. This response occurred more rapidly in R. decussatus but was of a lower magnitude than in V. pullastra. Activity of carbohydrases decreased rapidly in both species with starvation, although protease activity was maintained in R. decussatus. Recovery after the end of starvation was not similar in both species; while R. decussatus attained similar growth rates and enzyme activities to those measured prior to nutritional stress, V. pullastra only recovered 50% of its initial values. For both species of bivalves it can be concluded that digestive enzymes, and more specifically amylase, could be used as indicative of their nutritional condition.