Isolation and characterization of Aeromonas species from an eutrophic tropical estuary

Isolation and characterization of Aeromonas species were undertaken in the Ebrié lagoon (Abidjan, Côte d’Ivoire) over a one year period. Overall 63% of 501 water samples were identified as positive for Aeromonas spp. A. sobria, A. hydrophila and A. caviae represent respectively 49.20%, 20.63% and 30.15% of the positive samples. Thirty nine of the positive samples were recovered from surface water samples; while 24 bottom water samples were positive for Aeromonas. Aeromonas species are most frequently (82.53) present in the Ebrié lagoon urban area during the rainy and flood seasons when the salinity is low and below 10‰. A lower isolation frequency is noticed during the dry season when water salinity is over 10‰. Finally, a higher isolation frequency occurred in samples with highest counts of Escherichia coli.     

Trace metals in fucoid algae and purple sea urchins near a high Arctic lead/zinc ore deposit

Seaweed and urchins accumulate Fe and Zn to higher concentrations near a lead/zinc ore body than further away. The concentration of three metals (Cu, Fe and Zn) in seaweed increase with age of tissue. As and Cd are lowest in tissue of intermediate age, suggesting a reversible bonding mechanism for the two elements.Concentrations of Fe in whole urchins, and Zn in the gonads, are higher than concentrations in Fucus tips, which represent a lower level in the food web. Fe is higher in whole sea urchins than in their gonads. For As and Zn the relationship is reversed.     

Etropus crossotus, an annual flatfish species; age and growth of the fringed flounder in South Carolina

Fringed flounder, Etropus crossotus, a small flatfish common along the southeastern coast of the US, were collected along the South Carolina coast in the fall of 1992, and in the North Inlet estuary from August 1992 until July 1994. Standard length was 82% of total length and the relationship between standard length and wet weight showed near-isometric growth with no significant differences between males and females. Dry weight was 21% of wet weight and ash-free dry weight 14% of wet weight with no significant differences between sexes. Daily increments in the sagittal otoliths were used for age determination. No annual structures could be identified. The maximum age was about 14.5 months. Age-at-length was not different between the sexes. The relationship between age and length was exponential, with age (in days) equalling an e power of 4.242 + 0.135 × standard length (in cm). Back-calculated hatch dates and catches of early juveniles indicated a spawning season that lasted from March to October. Females started to be sexually mature at 7 to 7.5 cm standard length, but 50% maturity was reached at 8 to 8.5 cm. Females could grow to that size within one spawning season. Individuals hatched early in the spawning season could produce eggs in the latter portion of that season's spawning period.     

On poloidal mode coupling in the continuous spectrum of 2D equilibria

The continuous spectrum of linear ideal MHD is determined analytically in 2D magnetostatic models for coronal loops and arcades by means of a perturbation expansion. Poloidal mode coupling, induced by non-circularity of the cross-sections of the magnetic surfaces and/or variation of the plasma density along the magnetic field lines, is shown to occur in first order. The coupling is most pronounced on and near rational surfaces for particular poloidal and toroidal mode numbers and produces gaps in the continuous spectrum of ideal MHD.     

Arsenic in marine organisms from West Greenland

Natural background levels of trace metals in marine organisms from a West Greenland inlet were studied during the summer of 1972 and 1973. Dry weight arsenic levels ranged from 11.1 to 307 ppm in fish fillets and from 7.6 to 512 ppm in fish livers. Prawns had arsenic levels ranging up to 80.2 ppm, contrasting with 6.0 ppm in planktonic copepods. The results suggest that in certain marine organisms organo-arsenics predominate and are less toxic than inorganic arsenic compounds.     

Paleoindian bison procurement and paleoenvironments in Middle Park of Colorado

The Upper Twin Mountain Site, located within a geologic slump scar at 2548 m altitude, provides significant information on Paleoindian bison procurement. As the highest known Paleoindian bison bone bed, the site contained the partial skeletal remains of at least 15 adult Late Pleistocene bison (B. antiquus), Goshen projectile points, and debitage. Radiocarbon dates of 10,240±70 and 10,470±50 yr B.P. place Upper Twin Mountain midway between the northern Plains Goshen and southern Plains Plainview sites. With a late fall or early winter mortality of the bison and the presence of only local stone, year round occupation of Middle Park is indicated. Analysis of the geology, soil, and pollen, from the archaeological and nonarchaeological deposits at the Upper Twin Mountain and elsewhere in the region are used to describe the paleoenvironmental conditions in Middle Park. © 1999 John Wiley & Sons, Inc.     

Modelling the dynamics of snow cover, soil frost and surface ice in Norwegian grasslands

Studying the winter survival of forage grasses under a changing climate requires models that can simulate the dynamics of soil conditions at low temperatures. We developed a simple model that simulates depth of snow cover, the lower frost boundary of the soil and the freezing of surface puddles. We calibrated the model against independent data from four locations in Norway, capturing climatic variation from south to north (Arctic) and from coastal to inland areas. We parameterized the model by means of Bayesian calibration, and identified the least important model parameters using the sensitivity analysis method of Morris. Verification of the model suggests that the results are reasonable. Because of the simple model structure, some overestimation occurs in snow and frost depth. Both the calibration and the sensitivity analysis suggested that the snow cover module could be simplified with respect to snowmelt and liquid water content. The soil frost module should be kept unchanged, whereas the surface ice module should be changed when more detailed topographical data become available, such as better estimates of the fraction of the land area where puddles may form.     

Imaging the recent sediment dynamics of the Galicia Bank region (Atlantic,NW Iberian Peninsula)

Multibeam bathymetry, high resolution multi-channel, and very high resolution single-channel (3.5 kHz) seismic records were used to depict the complex geomorphology that defines the Galicia Bank region (Atlantic, NW Iberian Peninsula). This region (≈620–5,000 m water depth) is characterized by a great variety of features: structural features (scarps, highs, valleys, fold bulges), fluid dynamics-related features (structural undulations and collapse craters), mass-movement features (gullies, channels, mass-flow deposits, slope-lobe complexes, and mass-transport deposits), bottom-current features (moats, furrows, abraded surface, sediment waves, and drifts), (hemi)pelagic features, mixed features (abraded surfaces associated to mixed sediments) and bioconstructions. These features represent architectural elements of four sedimentary systems: slope apron, contouritic, current-controlled (hemi)pelagic, and (hemi)pelagic. These systems are a reflection of different sedimentary processes: downslope (mass transport, mass flows, turbidity flows), alongslope (bottom currents of Mediterranean Outflow Water, Labrador Sea Water, North Atlantic Deep Water, and Lower Deep Water), vertical settling, and the interplay between them. The architectural and sediment dynamic complexities, for their part, are conditioned by the morphostructural complexity of the region, whose structures (exposed scarps and highs) favor multiple submarine sediment sources, affect the type and evolution of the mass-movement processes, and interact with different water masses. This region and similar sedimentary environments far from the continental sediment sources, as seamounts, are ideal zones for carrying out submarine source-to-sink studies, and can represent areas subject to hazards, both geologic and oceanographic in origin.     

Assessment of Cyto‐ and Genotoxic Effects of a Variety of Chemicals Using Saccharomyces cerevisiae

Saccharomyces cerevisiae as the most simple eukaryotic organism is broadly accepted as a laboratory model organism. For the detection of potential toxic effects of pure compounds and complex composed samples like wastewater a miniaturised short‐term in vitro cyto‐ and genotoxicity screening assay was developed. The assay based on genetically modified S. cerevisiae cells deleted in the prominent drug efflux transporters Pdr5, Snq2, and Yor1 that facilitate pleiotropic drug resistance. The yeast strain devoid of these proteins that mediate the efflux of structurally diverse hydrophobic compounds exhibited an increased sensitivity to a variety of organic compounds. The DNA damage inducible RAD54 promoter fused to a yeast optimized derivative of the GFP (green fluorescent protein) gene from the jelly fish Aequorea victoria served as an indicator of DNA damage in this strain. Various pure compounds including the direct‐acting genotoxins methyl‐N‐nitro‐N‐nitrosoguanidine (MNNG), 4‐nitroquinoline‐N‐oxide (4‐NQO), methyl methanesulfonate (MMS) and hydroxyurea as well as the heavy metals cadmium and chromium(VI), the insecticide lindane and the central nervous system stimulant caffeine were tested exhibiting dose dependent induction of green fluorescence. All compounds were in parallel examined for chronic toxicity. A bioassay detecting simultaneously geno‐ and cytotoxic effects of potential toxicants in a single assay can be an important tool with a variety of applications in environmental monitoring and aquatic ecotoxicology. By partial automation and miniaturisation to microtitration scale this bioassay enables sensitive and fast biomonitoring for a multitude of samples.