Isolation and identification of a bacterium from marine shrimp digestive tract: A new degrader of starch and protein

It is a practical approach to select candidate probiotic bacterial stains on the basis of their special traits. Production of digestive enzyme was used as a trait to select a candidate probiotic bacterial strain in this study. In order to select a bacterium with the ability to degrade both starch and protein, an ideal bacterial strain STE was isolated from marine shrimp (Litopenaeus vannamei) intestines by using multiple selective media.The selected isolate STE was identified on the basis of its morphological, physiological,and biochemical characteristics as well as molecular analyses. Results of degradation experiments confirmed the ability of the selected isolate to degrade both starch and casein. The isolate STE was aerobic, Gram-negative, rod-shaped, motile and non-spore-forming, and had catalase and oxidase activities but no glucose fermentation activity. Among the tested carbon/nitrogen sources, only Tween40, alanyl-glycine, aspartyl-glycine, and glycyl-l-glutamic acid were utilized by the isolate STE. Results of homology comparison analyses of the 16S rDNA sequences showed that the isolate STE had a high similarity to several Pseudoalteromonas species and, in the phylogenetic tree, grouped with P. ruthenica with maximum bootstrap support (100%). In conclusion, the isolate STE was characterized as a novel strain belonging to the genus Pseudoalteromonas.This study provides a further example of a probiotic bacterial strain with specific characteristics isolated from the host gastrointestinal tract.     

太湖梅梁湾上空颗粒态磷浓度2003年春季的变化

太湖水体氮磷营养盐的研究比较多,但对大气营养物质的输入研究方面仍鲜有报道.通过定期采集太湖梅梁湾地区上空颗粒物,测定颗粒中各种形态磷(可溶性无机磷、有机磷、难溶性磷)浓度,探讨大气磷输入对梅梁湾水质的磷的贡献.结果表明2003年春季梅梁湾上空平均颗粒态磷浓度分别为0.157μg/m3,其中水溶性无机磷的含量在15.6%-51.0%.最后估算了春季大气总磷输入量,春季两次采样周期中大气磷沉降通量相差不大.进一步估算了大气总磷的沉降通量最大为0.57kg(hm2·a)(6.84t/a),显示大气沉降对太湖水体的影响.     

Hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) in riverine and estuarine sediments of the Pearl River Delta in southern China, with emphasis on spatial variability in diastereoisomer- and enantiomer-specific distribution of HBCD

Hexabromocyclododecane (HBCD) and tetrabromobisphenol A (TBBPA) were measured in surface sediments in the Pearl River Delta, southern China. The concentrations ranged from 0.06 to 304 ng/g dry weight for TBBPA and from 0.03 to 31.6 ng/g for total HBCD. The correlations between the TBBPA and HBCD concentrations were significant in rivers (Dongjiang, Zhujiang, Beijiang, and Dayanhe Rivers) with local input sources but not significant in the Xijiang River and Pearl River Estuary without local sources. HBCD was dominated by γ-HBCD (averaged 52.5-75.0%), whereas α-HBCD contributions were relatively high in sediments from the urban areas. The enantiomeric fractions of γ-HBCD in the sediments (averaged from 0.431 ± 0.035 to 0.479 ± 0.010) differed significantly from that in HBCD technical product except for samples from the e-waste area (Dayanhe River, 0.488 ± 0.091). This suggests that enantioselective biotransformation of HBCD occurs in the aquatic environment in this region.     

Features of the Distribution of Abnormal Gasgeochemical Fields in the Red River Rift (Gulf of Tonkin,South China Sea)

Doklady Earth Sciences - This paper reports the results of the third Russian–Vietnamese expedition (V.I. Il'ichev Pacific Oceanological Institute, Far East Branch, Russian Academy of...     

新疆独山子ＧＰＳ形变测量与断层活动性研究

1995 年5 月2 日独山子南发生MS5.8 地震后,对独山子南部山区开展了GPS测量,自1995～1997 年共进行3 次复测。独山子GPS网共布设测点13 个,跨附近的一些活动构造断裂。所有观测量用随机软件进行了平差处理,最大相对误差为2.4×10- 7。独山子地区的活动断层总的活动特点是以逆断或逆掩方式为主,主断层面多朝南倾或近直立,四条主要断裂在两年来的活动速率为0.4～5m m /a。     

富营养化浅水湖泊藻源性湖泛的短期数值预报方法——以太湖为例

本文建立了一种富营养化浅水湖泊藻源性湖泛的短期数值预报方法.选取表征藻源性湖泛的代表性指标叶绿素a和溶解氧浓度作为预测变量,以天气预报中的风场为驱动力,求解浅水湖泊三维水动力水质耦合数值模型,计算未来3 d浅水湖泊叶绿素a和溶解氧浓度的时空分布,然后结合未来3 d的气象因子信息建立经验公式,计算湖泛易发水域发生湖泛的概率,并进一步确定湖泛发生位置和面积.以太湖为例,采用构建的方法于2013 2014年夏、秋季对太湖7段湖泛易发水域的湖泛发生概率及发生面积进行未来3 d的预测预报,预报正确率在80%以上.     

Estimation of internal nutrient release in large shallow Lake Taihu,China

Based on field investigation of wave, sediment suspension and the changes in nutrient concentration of the water column in Lake Taihu, China, we proposed two release models to quantify nutrient release under static and dynamic conditions, respectively. Under static conditions, nutrient release from sediments to the overlying water mainly depends on chemical diffusion induced by concentration gradient, in which the nutrient release is controlled by the temperature, dissolved oxygen concentration in the sediment-water interface, oxidation-reduction potential and the concentration difference between porewater and overlying water. Under dynamic condition (or disturbed condition), both dissolved and particulate nutrients in sediments are released into the water column because of wind-induced sediment suspension. The amount of nutrient release under dynamic conditions is larger than that under the static condition. The release of dissolved nutrients, however, does not increase because the wind induced turbulence made oxidation of metallic elements such as Fe (ferric iron), Mn which are capable of precipitating soluble reactive phosphate (SRP). Under dynamic conditions, therefore, the release of total phosphorus (TP) increases dramatically but the release of SRP is close to those under static conditions. In sediments of Lake Taihu, high Fe content leads to a high ratio of Fe to P contents in sediments (Fe:P ratio). Under dynamic conditions, therefore, nutrient release is controlled by the intensity of disturbance, sediment consolidation and nutrient content in sediments. As for dissolved nutrients, especially SRP, the release is also controlled by the intensity of dynamic re-oxidation, Fe content in sediments and nutrient concentration gradient between porewater and overlying water. Based on these two release modes, the release flux in Lake Taihu has been estimated. In the static condition (i.e. laboratory experimental condition), total release of NH₄ ⁺-N for whole lake is ca. 10,000 ton/a, and PO₄ ³⁻-P is ca. 900 ton/a. In the dynamic condition, nutrient release following sediment suspension was estimated according to three different intensities of wind forcing which were defined as “calm” (wind speed is less than 2 m/s), “gentle” (wind speed is greater than 2 m/s and less than 6 m/s) and “gust” (wind speed is greater than 6 m/s). The release rate in the condition of “calm” was estimated in terms of the nutrient release in the laboratory experimental static condition; whereas the release rate in conditions of “gentle” and “gust” was estimated in terms of measurement during sediment resuspension conducted in flume experiments. With the observation of wind velocity and frequency in 2001, each type of wind forcing took the frequency of 12%, 82% and 6% for “calm”, “gentle” and “gust”, respectively. The yearly release of nitrogen was 81,000 ton and phosphorus was 21,000 ton, which is about 2–6 folds of annual external loading, respectively.

     

太湖梅梁湾藻类及相关环境因子逐步回归统计和蓝藻水华的初步预测

以太湖梅梁湾1992－1999年的连续监测资料为基础,运用多元逐步回归统计方法,选择水温等15项环境理化因素与藻类叶绿素a、藻类总生物量和微囊藻生物量等3项生物因素进行逐步回归分析,找出与生物因素显著相关的环境因子,建立多元逐步回归方程,预测梅梁湾藻类生物量的变化情况,初步进行了梅梁湾蓝藻水华的预测预报,结果显示,水温和总磷为梅梁湾藻类总生物量的显著相关因子,水温、硝态氮和总氮为微囊藻一物量的显著相关因子。     

青海湖全新世稳定暖湿期的古水量平衡估算

各种地貌学、沉积学等证据揭示了青海湖流域在全新世大暖期是一个温暖湿润的环境,特别是距今6000～7200a.B.P.的稳定暖湿期,湖水位远高于现代.利用内陆湖泊的水热平衡原理,构造了青海湖古水量平衡计算模型.计算结果表明,在全新世稳定暖湿期,流域内的降水量、蒸发量及径流量分别比现代高32.0%、14.0%及67.0%.     

黄河流域近40年日照百分率的气候变化特征

利用黄河流域及其周边146个气象站1960--2000年逐月日照百分率资料，研究分析了黄河流域日照百分率的气候变化趋势。结果表明，就整个流域平均而言，日照百分率呈明显下降趋势，黄河流域年平均日照百分率在20世纪90年代较60年代下降了2．49％；日照百分率的下降主要表现在夏季和冬季，春季和秋季下降不明显。对日照百分率气候变化的空间分析表明，除极少数台站的日照百分率呈略有上升趋势外（主要出现在流域上游），黄河流域日照百分率的下降表现得非常明显，遍布整个流域的中、下游。