大鹏湾潮流数值计算

应用分步杂交方法建立了大鹏湾边界潮流数值模型，并对其进行了模拟计算，重现了该区域潮波及潮流的分布规律，并根据实测资料对其进行了验证，从而可系统地了解这一海区的潮波系统及潮流场等海洋要素的分布情况。     

亚热带沿海景观的旅游保护性开发及建设探讨--以福建沿海为例

依据地貌学和风景学的角度,福建沿海景观以闽江口和九龙江口为界可分为3个景观亚区,并具有多样性与复杂性、动态性与脆弱性和关联性与分异性等特征.本文运用景观生态的原理研究沿海景观的构成与格局,在沿海景观的分类和特征概括的基础上,探讨福建沿海景观旅游开发与建设的模式.通过维护沿海景观系统的稳定,优化沿海各景观亚区的分布格局,提高沿海景观的整体舒适性和挖掘文化景观的内涵等应对措施以实现福建沿海旅游的科学发展.     

0102号台风"飞燕"移动路径特点的分析

2001年2号台风“飞燕”登陆早、强度大、移速快、突发性强给福建中部沿海地区带来巨大损失。本文利用常规资料及云图等资料对其登陆福建及后期路径北折的特点及成因进行分析，结果表明：200hPa散度、850hPa涡度及低层能量场对台风路径北折有比较好的预示作用，台风未来移向有沿着200hPa其中心附近辐散中心长轴方向、850hPa正涡度长轴方向及低层暖平流区和850hPa θse高能中心轴移动的趋势。     

合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

Calibration of JASON-1 Altimeter over Lake Erie

This article describes absolute calibration results for both JASON-1 and TOPEX Side B (TSB) altimeters obtained at the Lake Erie calibration site, Marblehead, Ohio, USA. Using 15 overflights, the estimated JASON altimeter bias at Marblehead is 58 ± 38 mm, with an uncertainty of 19 mm based on detailed error analysis. Assuming that the TSB bias is negligible, relative bias estimates using both data from the TSB-JASON formation flight period and data from 48 water level gauges around the entire Great Lakes confirmed the Marblehead results. Global analyses using both the formation flight data and dual-satellite (TSB and JASON) crossovers yield a similar relative bias estimate of 146 ± 59 mm, which agrees well with open ocean absolute calibration results obtained at Harvest, Corsica, and Bass Strait (e.g., Watson et al. 2003). We find that there is a strong dependence of bias estimates on the choice of sea state bias (SSB) models. Results indicate that the invariant JASON instrument bias estimated oceanwide is 71 mm, with additional biases of 76 mm or 28 mm contributed by the choice of Collecte Localisation Satellites (CLS) SSB or Center for Space Research (CSR) SSB model, respectively. Similar analysis in the Great Lakes yields the invariant JASON instrument bias at 19 mm, with the SSB contributed biases at 58 mm or 13 mm, respectively. The reason for the discrepancy is currently unknown and warrants further investigation. Finally, comparison of the TOPEX/POSEIDON mission (1992-2002) data with the Great Lakes water level gauge measurements yields a negligible TOPEX altimeter drift of 0.1 mm/yr.     

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.     

固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.     

Distribution and number change of the Littorinid(Gastropoda) on intertidal hard bed in the Jiulong Estuary, Fujian Province

-Among four species of Littorinid, Littorina scabra, L. brevicula, Nodiliitorina millegrana and N. pyramidalis pyramidalis, on the hard intertidal zone in the Jiulong Estuary, Fujian, L. scabra has the most extensive distribution both vertically and horizontally, and the highest anual average density. The uppermost limit of vertical distribution of N. pyramidalis pyramidalis is higher than the other three Littorinid. Desiccation is a main factor affecting the uppermost limit of vertical distribution of the Littorinid. The increasing wave action will raise the uppermost limit vertical distribution of Littorinid. With seasonal changes, the densities of the Littorinid also change in different tidal zonations. Difference in salinity is the main factor affecting the horizontal distribution of the Littorinid in the estuary.     

几种养殖鲍同工酶生化遗传的比较研究

运用聚丙烯酰胺凝胶电泳对我国主要养殖鲍(皱纹盘鲍、盘鲍及九孔鲍)12种同工酶(LDH、ME、MDH、GPI、PGM、IDH、IDDH、SOD、EST、ODH、ADH、AAT)26个基因座位进行了分析．结果表明：EST、PGM可以作为区分三种(亚种)鲍的生化遗传标记；在所分析的位点中，未发现多态现象，表明三种养殖鲍杂合子缺失严重，其主要原因可能在于哑基因、近交与自然选择。     

摄食水平和性别对稀有鮈鲫生长和能量收支的影响

于1995年10月采集本所自繁有ju鲫（约1.03g/ind)，在30℃水温下进行摄食－生长实验，研究了从饥饿到饱食5个摄食水平对不同性别稀有ju鲫的生长和能量收支的影响，结果表明，鱼体干物和能量含量不受摄食率和性别的影响，随着摄食率的增加，雌鱼的湿重，干重和能量特定生长率均呈双对数增加，雄鱼湿重特定生长率呈双对数增加，而干物质和能量特定生长率呈指增加，雄鱼生长速度及摄食率低于雌鱼，随着摄食率的增加，食物能损失于粪便中的比例均呈上升趋势，损失于排泄物中的比例变化不大，呈下降趋势，用于代谢的比例呈下降趋势，用于生长的比例呈上升趋势，除饥饿组外，在各个摄食水平，雌鱼食物能用于代谢的比例较雄鱼低，而用于生长的比例较雄鱼高。