A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.     

天津近海小型底栖动物丰度研究

2006年7月至2007年10月在渤海湾天津近海的15个站位,分春、夏、秋、冬4个航次进行了小型底栖动物丰度的调查.通过对未受扰动沉积物样品中的生物分析,共采集到线虫、桡足类、多毛类、介形类、寡毛类、双壳类、动吻类等小型底栖动物类群和少量未鉴定实体,其中线虫为优势类群,占总丰度的90%以上.调查海域小型底栖动物丰度春、夏、秋、冬依次为(405.4±154.8)ind/10cm2,(417.6±38.6)imd/10 cm2,(161.6±64.5)ind/10cm2和204.7±69.7ind/10 cm2,区内的分布以中部海域居多.小型底栖动物丰度值存在季节变化,春季和夏季平均丰度值较高,夏季略高于春季;秋冬季值偏低.小型底柄动物多分布于沉积物0～5 cm层次,占总量的85.9%～92.9%.春季和秋季的小型底栖动物丰度值与沉积物叶绿素a含量显著相关.与我国近海海域研究资料比较显示,目前渤海湾天津近海小型底栖动物丰度值略低.     

准噶尔盆地中部Ⅲ区块原油甾烷异常分布特征与成因

对准噶尔盆地中部Ⅲ区块的3类原油或油砂样品抽提物饱和烃进行了气相色谱-质谱分析,研究了原油饱和烃中甾烷类化合物的分布特征,并对其成因做了初步探讨。结果表明：3类原油的甾烷分布特征具有一定的相似性,C27规则甾烷相对丰度明显低于C28、C29;C27、C28、C29异构体αββ型相对丰度明显高于ααα型（C28αββ（20S）或C29αββ（20R）丰度最高）;但这3类原油的成因是不一致的,其影响因素主要包括热演化、生物降解、运移分馏以及古环境或岩性4个方面,其中第1、3类原油主要以热演化作用与运移作用为主,第1类原油还可能受到古环境或岩性影响,两者均不受生物降解的影响,第2类原油则受到热演化、运移作用及生物降解的共同影响。     

南海南部近50万年来沉积物特点及古环境意义

对南海南部MD05-2897孔沉积物的分析显示,该孔涵盖氧同位素1~12期,底界年龄约为50万年。碳酸钙含量曲线形态基本与底栖有孔虫氧同位素曲线平行,即碳酸钙高值对应氧同位素轻值,呈现典型的大西洋型碳酸钙分布模式。碳酸钙含量在冰消期的高值出现及含量变化均领先于氧同位素,证明低纬海区"碳酸钙泵"对大气二氧化碳浓度和温度的作用。粒度分析显示,3~5μm组分含量曲线的变化与氧同位素曲线基本平行,说明它主要受海平面升降变化控制,可作为海平面变化的间接指标。而1.5~2.5μm粒级颗粒主要通过河流的悬浮搬运,直接受亚洲夏季风及其所带来降雨的影响,可间接反映夏季风的强弱变化。南海南部深海沉积中的季风记录具有0.1 Ma偏心率周期,40 ka斜率周期,20 ka岁差和10 ka半岁差周期等丰富的频谱,显示出低纬度海区气候变化对轨道周期的良好响应。     

山东昌邑国家级海洋生态特别保护区生态环境现状及管护对策研究

山东昌邑国家级海洋生态特别保护区是目前国内唯一以柽柳林生态系统为主要管理和保护对象的国家级海洋保护区。为系统、全面地了解保护区内生态环境和保护物种的变化趋势,文章对山东昌邑国家级海洋生态特别保护区生态环境现状进行了监测与评价,并根据评价结果,分析了保护区保护与管理中存在的问题。研究表明,该保护区生态环境一直处于亚健康状态,为促进保护及管护,提出了加强保护区生态建设,改变保护区开发利用模式;加强能力建设,实现保护区动态监控;协调发展与保护的关系,实现资源可持续利用;大力开展柽柳生态修复,恢复柽柳的资源状况的对策建议。     

新型珊瑚海水混凝土力学性能试验研究

为了解决全珊瑚海水混凝土(coral aggregate seawater concrete,简称CASC)的脆性问题,采用碱式硫酸镁水泥(basic magnesium sulfate cement,简称BMSC)为胶凝材料和剑麻纤维为增韧材料制备了一种新型全珊瑚海水混凝土(NCASC),系统测定了基本力学性能,并与普通硅酸盐水泥制备的CASC、碱式硫酸镁水泥砂石混凝土(basic magnesium sulfate cement concrete,简称BMSCC)、普通砂石混凝土(ordinary portland cement concrete,简称OPC)以及普通轻骨料混凝土(lightweight aggregate concrete,简称LAC)的力学性能进行对比。结果表明:立方体抗压强度(f_(cu),m)在15~35 MPa范围内的NCASC的轴心抗压强度(f_c,m)、劈裂抗拉强度(f_(sp),m)与f_(cu),m之间具有较显著的线性关系;对于f_(cu),m为15~35 MPa的混凝土,相同强度NCASC的f_c,m高于OPC、LAC及BMSCC,其f_c,m与f_(cu),m比值最大,大致在0.85~0.96之间,NCASC的f_(sp),m比OPC和LAC高,但比BMSCC低,且随着强度的提高,OPC与NCASC的f_(sp),m间的差距在增大,而LAC和BMSCC与NCASC的f_(sp),m间的差距在缩小;在强度等级C15~C55的范围中,NCASC和CASC的f_(sp),m、f_c,m与f_(cu),m之间的关系基本可以采用相同的线性回归方程来描述,利用这两个回归线性方程得到对应于标准强度等级的CASC和NCASC的f_(sp),m和f_c,m数据,为CASC力学性能研究与结构设计提供参考依据。     

土壤氡析出率活性炭累积吸附法

为解决大面积多点快速土壤氡析出率测量问题, 设计开发并进行了土壤氡析出率活性炭累积吸附法的方法技术研究.在参考氡析出率装置和野外实验数据的基础上, 进行了测量装置及材料的选择、装置刻度、野外累积暴露时间、活性炭盒吸附氡有效衰变常数等研究.通过2002-2003年22次重复观测, 北京西北郊区粘土类土壤氡析出率平均值为20.15mBq.m-2.s-1, 最大值和最小值分别为26.75和13.49mBq.m-2.s-1, 标准偏差为3.55mBq.m-2.s-1.与仪器静电收集法比对结果表明, 两种方法的相对误差小于±10%.实验结果表明: 活性炭方法测量结果稳定可靠, 简便易行, 适用于全国大面积土壤氡析出率调查.      

Spatio-temporal Variation of Soil Respiration and Its Driving Factors in Semi-arid Regions of North China

Soil respiration (SR) is the second-largest flux in ecosystem carbon cycling. Due to the large spatio-temporal variability of environmental factors, SR varied among different vegetation types, thereby impeding accurate estimation of CO₂ emissions via SR. However, studies on spatio-temporal variation of SR are still scarce for semi-arid regions of North China. In this study, we conducted 12-month SR measurements in six land-use types, including two secondary forests (Populus tomentosa (PT) and Robinia pseudoacacia (RP)), three artificial plantations (Armeniaca sibirica (AS), Punica granatum (PG) and Ziziphus jujuba (ZJ)) and one natural grassland (GR), to quantify spatio-temporal variation of SR and distinguish its controlling factors. Results indicated that SR exhibited distinct seasonal patterns for the six sites. Soil respiration peaked in August 2012 and bottomed in April 2013. The temporal coefficient of variation (CV) of SR for the six sites ranged from 76.98% to 94.08%, while the spatial CV of SR ranged from 20.28% to 72.97% across the 12-month measurement. Soil temperature and soil moisture were the major controlling factors of temporal variation of SR in the six sites, while spatial variation in SR was mainly caused by the differences in soil total nitrogen (STN), soil organic carbon (SOC), net photosynthesis rate, and fine root biomass. Our results show that the annual average SR and Q₁₀ (temperature sensitivity of soil respiration) values tended to decrease from secondary forests and grassland to plantations, indicating that the conversion of natural ecosystems to man-made ecosystems may reduce CO₂ emissions and SR temperature sensitivity. Due to the high spatio-temporal variation of SR in our study area, care should be taken when converting secondary forests and grassland to plantations from the point view of accurately quantifying CO₂ emissions via SR at regional scales.     

近场MVDR聚焦波束扫描声图定位方法研究

声图测量技术是一种适用于近场的高精度被动定位技术,可以用来测量舰船辐射噪声源位置的空间分布。声图测量的核心算法是聚焦波束形成。算法的分辨力与阵长、信号处理频段以及目标距离有关。当阵长一定、测量区范围给定时,算法对低频声源的分辨力较差。MVDR波束形成法可较常规波束形成获得更高的分辨力,降低旁瓣。采用MVDR与聚焦波束形成相结合的方法可提高对舰船各部位辐射噪声源的分辨力。