利用密度和核磁共振测井分析Mallik 5L-38井的天然气水合物饱和度

以前的研究表明密度和核磁共振测井数据可用来精确计算储层的天然气饱和度。传统的气体储层中开发的用于天然气饱和度计算的密度-磁共振法也可用于天然气水合物饱和度分析。在不使用复杂方程或假设的情况下，基于经验方程，密度-磁共振方法可得到精确的地层孔隙度、气体和（或）天然气水合物饱和度。本文通过密度-磁共振响应方法来识别Mallik 5L-38井所穿沉积剖面中的天然气水合物带。结果证明密度-磁共振方法可以计算经气体校正后的孔隙度和精确的天然气水合物饱和度。     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

鳖配合饲料喂试验简报

鳖的养殖属新兴水产业，养殖水平尚待提高，本着为广大养殖业者服务的宗旨，研制开发了“大昌”牌鳖配合饲料。本文简报了中华鳖饲料１７０ｄ的饲喂试验初步成果：稚鳖平均饲料系数为１．８９，幼鳖平均饲料系数为２．４０。没有出现鳖的营养缺乏症。     

坛紫菜中藻胆蛋白的性质与化学组成研究

坛紫菜(于1988年采自青岛太平角海区人工养殖筏上)的水溶性色素粗提物经过硫酸铵沉淀和羟基磷灰石(HA)柱层析后,分离出藻蓝蛋白(RPC)、藻红蛋白(RPE)和变藻蓝蛋白(APC)。在中性介质中,其吸收光谱和荧光发射光谱与文献报道基本一致;但在酸性(pH=3)或碱性(pH=12)介质中,吸收光谱较在中性介质中有明显改变,原有的荧光性质也消失。RPC和APC只分离到一种聚集体,但RPE有两种不同的聚集体。用SephadexG—100凝胶过滤方法测量藻胆蛋白的分子量分别为:RPC117000,APC112000,小分子RPE38000,大分子RPE232000。对三种藻胆蛋白的氨基酸分析的结果表明,三种藻胆蛋白中都是酸性氨基酸的含量大于碱性氨基酸的含量。     

不同生长期坛紫菜中藻胆蛋白的含量变化

于１９９０年９－１２月在青岛海区人工养殖筏采集坛紫菜，提取混合藻胆蛋白，第其经羟基磷灰石柱层析，分离出Ｒ－藻红蛋白（ＲＰＥ）、Ｒ－藻蓝蛋白（ＲＰＣ）和变藻蓝蛋白（ＡＰＣ）。测定了３个不同生长发育阶段的北移坛紫菜中各藻胆蛋白的含量，并与南方坛紫菜做了比较研究。结果表明，坛紫菜生长初期和盛期藻胆蛋白含量较高；到末期大幅度降低，降低的幅度为ＲＰＥ〉ＲＰＣ〉ＡＰＣ。各种藻胆蛋白的光谱特性不随藻体的生长发育     

长江口区浮游动物生态研究

长江口区终年有大量长江淡水流入,营养盐丰富,并且泥沙含量较大,透明度低,盐度变化幅度大(3-33)。 长江口区是我国重要渔场之一,凤鲚、刀鲚、银鱼、鲻、鲳等均在此产卵,许多海产鱼类的仔、幼鱼也在此索饵、栖息。因此长江口区浮游动物生物量的分布与其附近渔场有密切关系。     

用音响训练鱼类

鱼类,时而群居,时而分散,给捕获带来一定的困难。为使其按照人们的意原聚集和分散,许多国家的研究人员尝试用音响训练放流幼苗,并对野生鱼类进行训练,将其限定在某一特定水域,便于成熟后捕获,为人类创造更丰富的海洋食品。     

Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants

The increasing emphasis on the assessment and monitoring of estuarine ecosystems has highlighted the need to deploy appropriate biological indices for these locations. Fish diseases and histopathology, with a broad range of causes, are increasingly being used as indicators of environmental stress since they provide a definite biological end-point of historical exposure. This study reports on the histopathological alterations observed in selected organs and tissues of three species of estuarine fish (Platichthys flesus, Pomatoschistus minutus and Zoarces viviparus), captured from four British estuaries (the Tyne, Tees, Mersey and Alde), differently impacted by contaminants, including PAHs. A biannual sampling regime was used to identify the important seasonal variations that occur in terms of the observed biological effects. Inflammatory lesions and hepatocellular fibrillar inclusions attained their highest prevalence in P. flesus captured from the Tyne, Tees and Mersey. The presence of pre-neoplastic and neoplastic toxicopathic lesions was highest in P. flesus captured from these sites, when compared to fish from the Aide reference site. In particular, the prevalence of hepatic foci of cellular alteration (up to 43.3%) and hepatocellular adenoma (up to 10%) were highest in P. flesus captured from the Mersey estuary. Intersex (ovotestis) was only recorded in male P.flesus captured from the Mersey estuary (up to 8.3%) and from male Z. viviparous captured from the Tyne estuary (25%). Pathologies associated with the gill and the kidney were also most prevalent in fish captured from the Tyne, Tees and Mersey estuaries. This study has successfully applied histopathology to an estuarine monitoring program, both for the recording of toxicopathic lesions in the liver and other organs, and for the detection of the endpoint of endocrine disruption, intersex. As such, it provides a powerful integrative tool for the assessment of biological effects of contaminants in these environments.     

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。     

上海地区水6井第四纪有孔虫复合分异度与古环境分析

作者通过对水6井有孔虫复合分异度H(S)、优势种、特殊属种生态及介形、孢粉等化石综合分析,确定7个海进层位,对上海地区古环境变迁提出新的看法,并为我国东部第四系划分和对比提供依据.