Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China （LCDV-cn） genome

An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene. Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309)     

Climatic and geographic factors affect ecosystem multifunctionality through biodiversity in the Tibetan alpine grasslands

Ecosystem multifunctionality(EMF), the simultaneous provision of multiple ecosystem functions, is often affected by biodiversity and environmental factors. We know little about how the interactions between biodiversity and environmental factors affect EMF. In this case study, a structural equation model was used to clarify climatic and geographic pathways that affect EMF by varying biodiversity in the Tibetan alpine grasslands. In addition to services related to carbon, nitrogen, and water cycling, forage supply, which is related to plantproductivity and palatability, was included in the EMF index. The results showed that 72% of the variation in EMF could be explained by biodiversity and other environmental factors. The ratio of palatable richness to all species richness explained 8.3% of the EMF variation. We found that air temperature, elevation, and latitude all affected EMF, but in different ways. Air temperature and elevation impacted the aboveground parts of the ecosystem, which included plant height, aboveground biomass, richness of palatable species, and ratio of palatable richness to all species richness. Latitude affected EMF by varying both aboveground and belowground parts of the ecosystem, which included palatable speciesrichness and belowground biomass. Our results indicated that there are still uncertainties in the biodiversity–EMF relationships related to the variable components of EMF, and climatic and geographic factors. Clarification of pathways that affect EMF using structural equation modeling techniques could elucidate the mechanisms by which environmental changes affect EMF.     

Analysis of spatial clustering optimization

Spatial clustering is widely used in many fields such as WSN (Wireless Sensor Networks), web clustering, remote sensing and so on for discovery groups and to identify interesting distributions in the underlying database. By discussing the relationships between the optimal clustering and the initial seeds, a clustering validity index and the principle of seeking initial seeds were proposed, and on this principle we recommend an initial seed-seeking strategy: SSPG (Single-Shortest-Path Graph). With SSPG strategy used in clustering algorithms, we find that the result of clustering is optimized with more probability. At the end of the paper, according to the combinational theory of optimization, a method is proposed to obtain optimal reference k value of cluster number, and is proven to be efficient.     

铁路缓和曲线的等间隔放样方法

介绍了铁路缓和曲线设置的目的、作用、基本原则和重要意义，阐述了等间隔放样缓和曲线的基本原理，给出了等间隔放样缓和曲线放样要素采集的基本方法，叙述了缓和曲线等间隔放样的工作过程。     

线/面Voronoi图的分解合并生成算法

Voronoi图生成算法受到计算效率或生长源类型的限制,难以支撑线/面生长源Voronoi图的构建。本文提出一种生成线/面生长源Voronoi图的分解合并算法,其主要过程是将线/面生长源离散为特征点表达,通过特征点交叉建立最近特征点对,并以最近特征点对Voronoi子区域的交来部分地代替线/面生长源的等距离边界,算法以前后迭代离散计算的Voronoi子区域面积差分作为条件,可有选择地将部分生长源置入迭代过程,使线/面生长源Voronoi子区域逐步调整并达到精度要求。     

浅谈地理国情监测外业调绘与核查的方法

地理国情监测外业调绘与核查是确保地理国情普查成果的真实性、准确性及提高地理国情普查成果质量的重要环节。介绍外业调绘与核查的目的、流程、准备工作及外业调绘与核查的内容,总结外业调绘与核查时应注意的问题,为保证地理国情监测外业任务的顺利开展提供参考。     

海岸线及其测绘技术探讨

海岸线是人类所使用的最古老的边界线之一,然而,由于其动态特征,加之当今社会对海岸带的需求越来越多,这条边界线的位置存在争议,因而,如何使用客观手段测绘海岸线尤为重要。文中针对海岸线定义及其位置确定不明确的现状,指出海岸线定义存在的问题,给出完善定义的意见;分析海岸线常规的测绘方法,探讨海岸线精确测绘的新方法和技术。     

浅谈北京市园林景观设计前期地形图测绘

北京市园林景观设计对前期测绘图有着特殊的要求,特别是在树木的测绘方面,本文结合已完成的多处相关工程的作业经验,简要介绍了此类地形图的测绘方法及特殊要求,为此类工程提供了一套完整有效的作业方法进行参考。     

全站仪在日照测量中的应用

从日照测量的目的和任务出发,介绍全站仪在日照测量中的具体应用及注意事项,通过实例分析日照测量外业数据采集的原则和内业成图的要求,实践证明利用全站仪进行日照测量工作,具有低成本、高效率、易操作的优点.     

Revealing the spatiotemporal characteristics of the general public's panic levels during the pandemic crisis in China

The existing crisis management research mostly reveals the patterns of the public's panic levels from the perspectives of public management, sociology, and psychology, only a few studies have revealed the spatiotemporal characteristics. Therefore, this study investigates the spatial distribution and temporal patterns and influencing factors on the general public's panic levels using the Baidu Index data from a geographic perspective. The results show that: (1) The public's panic levels were significantly correlated with the spatial distance between the epicenter and the region of investigation, and with the number of confirmed cases in different regions when the pandemic began to spread. (2) Based on the spatial distance between the epicenter and the region, the public's panic levels in different regions could be divided into three segments: core segment (0–500 km), buffer segment (500–1300 km), and peripheral segment (>1300 km). The panic levels of different people in the three segments were consistent with the Psychological Typhoon Eye Effect and the Ripple Effect can be detected in the buffer segment. (3) The public's panic levels were strongly correlated with whether the spread of the infectious disease crisis occurred and how long it lasted. It is suggested that crisis information management in the future needs to pay more attention to the spatial division of control measures. The type of crisis information released to the general public should depend on the spatial relationship associated with the place where the crisis breaks out.