适宜浓度的维生素E促进半滑舌鳎生长激素基因的表达

本实验旨在探究维生素E对半滑舌鳎垂体组织中生长激素基因表达的影响。作者在每千克等氮等能的基础饲料中分别添加0、400和1 600mg的DL-α-生育酚乙酸酯(维生素E)投喂半滑舌鳎(Cynoglossus semilaevis)成鱼(464±2.6) g,进行为期8周的养殖实验;另外,在L-15培养基中添加0、18和54μmol/L的维生素E,对半滑舌鳎成鱼(464±2.6) g的垂体细胞进行为期3 d的体外原代培养实验。分别取垂体组织和原代细胞,通过荧光实时定量PCR分析其gh mRNA的相对表达量。实验结果表明:垂体组织中gh mRNA的相对表达量随着饲料中维生素E含量的增加而呈现先升高后下降的变化趋势,在400 mg/kg组时显著高于其他各组(P0.05);随着细胞培养液中维生素E浓度的升高, gh mRNA的相对表达量显著增加(P0.05)。由此可见,适宜浓度的维生素E能够促进半滑舌鳎垂体组织中生长激素基因的表达。     

东北三省城乡收入差距空间格局及其分异机制研究

借助多元逐步线性回归、GWR等模型方法,探讨1990年以来东北三省城乡收入差距空间格局、类型特征及其分异机制,最后提出城乡统筹可持续发展建议。结果表明： 1990年以来,东北三省城乡居民收入水平大幅提高,但大部分地区城镇居民可支配收入水平仍低于全国平均水平;城乡收入差距整体有所拉大,呈现出“中间高、两端低”的空间特征。 城乡收入差距空间分布格局受经济发展水平、工业拉动效应、服务业带动力和交通通达程度影响,其中经济发展水平对城乡收入差距由早期的正向拉大作用为主,逐步演变为负向抑制效应。 据此提出对策建议：深化改革开放,加快国有企业改革步伐,优化区域产业结构,提高服务业发展水平和比重,激发市场活力,破解制约区域经济发展的各种障碍;加快实施乡村振兴战略,促进乡村资源开发,开辟致富增收新路径。     

Evaluation of the integrated multi-satellite retrievals for global precipitation measurement over the Tibetan Plateau

The availability of high-resolution satellite precipitation measurement products provides an opportunity to monitor precipitation over large and complex terrain and thus accurately evaluate the climatic, hydrological and ecological conditions in those regions. The Global Precipitation Measurement(GPM) mission is an important new program designed for global satellite precipitation estimation, but little information has been reported on the applicability of the GPM’s products for the Tibetan Plateau(TP). The object of this study is to evaluate the accuracy of the Integrated Multi-Satellite Retrievals for GPM(IMERG) Final Run product under different terrain and climate conditions over the TP by using 78 ground gauges from April 2014 to December 2017. The results showed the following:(1) the 3-year average daily precipitation estimation in the IMERG agrees well with the rain gauge observations(R~2=0.58, P0.01), and IMERG also has a considerable ability to detect precipitation, as indicated by a high probability of detection(78%-98%) and critical success index(65%-85%);(2) IMERG performed better at altitudes from 3000 m to 4000 m with a small relative bias(RB) of 6.4%. Precipitation change was not significantly affected by local relief;(3) the climate system of the TP was divided into four climate groups with a total of 12 climate types based on the K?ppen climate classification system, and IMERG performed well in all climate types with the exception of the arid-desert-cold climate(Bwk) type. Furthermore, although IMERG showed the potential to detect snowfall, it still exhibits deficiencies in identifying light and moderate snow. These results indicate that IMERG could provide more accurate precipitation data if its retrieval algorithm was improved for complex terrain and arid regions.     

Selective feeding of bay scallop Argopecten irradians on phytoplankton community revealed by HPLC analysis of phytopigments in Bohai Sea,China

Understanding the feeding selectivity on phytoplankton by shellfish is currently a big challenge.In order to investigate the feeding behavior of bay scallop(Argopecten irradians)on phytoplankton,we compared its compositions of phytopigments in digestive glands with those in the surrounding seawater,and conducted five consecutive investigations between July and November 2016 in a bay scallop culture area along coast of Qinghuangdao City,northwest of the Bohai Sea,China.Phytopigments in four-size fractionated phytoplankton of seawater(micro-(20-200 μm);nano(L)-[10-20 μm];nano(S)-[2.7-10 μm],and pico-[2.7 μm])and digestive glands of A.irradians were examined to investigate the selective feeding of A.irradians.Results show that fucoxanthin and peridinin constituted the major part of taxonomically diagnostic carotenoids(TDCs)in the micro-and nano(L)-phytoplankton in seawater.Compared with total phytoplankton biomass of seawater(TPB,sum of the four sizes),a substantial decrease of fucoxanthin proportion to total DCs in digestive glands was observed while that of peridinin,19'-butanoyloxyfucoxanthin,alloxanthin and 19'-hexanoyloxy-fucoxanthin showed an obvious increase when those pigments were mainly confined to micro-sized phytoplankton(20-200 μm).However,zeaxanthin and prasinoxanthin were mainly confined to nano(s)-and pico-phytoplankton,of which the proportions in digestive glands were usually lower in TPB.The contribution of lutein to total DCs in digestive glands(with an average of 7.23%)increased compared with TPB of seawater(with an average of 0.63%)during all five sampling times.     

A comparison of three widely used GPS triple-frequency precise point positioning models

GPS Solutions - The EUREF Permanent Network (EPN) is a network of continuously operating GNSS stations installed throughout the European continent. The EPN Central Bureau (CB) performs the...     

人工冻土观测的冻点对应的土壤温度研究

基于2018年12月至2020年3月喀左、沈阳、辽阳、满洲里4个国家级地面气象站人工冻土器与测温式冻土自动观测仪观测的资料,对人工冻土观测获得的冻点与测温式冻土自动观测仪获得的相应深度的温度进行对比分析.结果表明:人工冻土器获取的冻点对应的土壤温度与0℃总体一致,又不完全重合;0—35 cm深度范围,冻点对应的温度变化...     

中国金红石矿床成矿地质条件及成矿远景

中国金红石矿床成因类型不一,控矿因素各异。通过对地层、构造(包括大地构造环境及褶皱)等控矿因素,区域变质作用、岩浆作用、热液作用、风化及沉积作用等成矿地质条件及矿床分布等规律的归纳与研究,提出了陕西石泉-镇坪、陕西丹凤-西峡、河南南召-方城、湖北枣阳-河南新县、湖北英山-潜山、江苏徐州-东海、山东栖霞-荣城、山西代县-河北涞水、四川会东9个金红石成矿远景区。并圈定出山东省荣城曲家、安徽凤阳板桥、湖北郧西土门—涧池、湖北七里坪大悟仙山、山西代县义成沟—羊廷寺、河北涞源司各庄、河北丰宁黄土梁等7个找矿靶区。     

Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated he...     

Interaction of water waves with vertical cylinders using null-field integral equations

The scattering of water waves by bottom-mounted vertical circular cylinders is solved by using the null-field integral equations in conjunction with degenerate kernels and Fourier series to avoid calculating the Cauchy and Hadamard principal values. In the implementation, the null-field point can be exactly located on the real boundary owing to the introduction of degenerate kernels for fundamental solutions. An adaptive observer system of polar coordinates is considered to fully employ the properties of degenerate kernels. For the hypersingular equation, vector decomposition for the radial and tangential gradients is carefully considered. This method can be seen as a semi-analytical approach since errors attribute from the truncation of Fourier series. Neither hypersingularity in the Burton and Miller approach nor the CHIEF concept was required to deal with the problem of irregular frequencies. Five advantages of free of calculating principal value, well-posed algebraic system, convergence rate of exponential order, meshfree and elimination of boundary-layer effect, are achieved by using the present approach. Numerical results are given for the forces and free-surface elevation around the circular boundaries. Also, the near-trapped behavior arisen from the physical resonance is detected. A general-purpose program for water wave impinging several circular cylinders with arbitrary number, radii, and positions was developed. Several examples of water wave structure interaction by vertical circular cylinders were demonstrated to see the validity of the present formulation.