Evaluating the use of 3'-(p-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions

Background  

Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(p-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions.     

共反射面元叠加技术在南海北部莺—琼盆地中的应用

随着莺—琼盆地油气勘探开发的不断深入,2 700m以下的中深层地震资料品质迫切需要提高,而改善信噪比提高成像质量则是需要深入研究的重点内容之一。基于共反射面元(CRS)叠加在改善中深层地震资料信噪比方面具有理论和技术应用优势,由于考虑了反射层的局部特征和第一菲涅耳带内的全部反射,对复杂地质问题的适应性得以增强,真正实现了高信噪比叠加,从而改善中深层低信噪比区的成像质量。通过理论研究分析、模型试处理及莺—琼盆地实际地震资料处理中具体的应用与试验,取得了明显的效果,即该区中深层地震资料信噪比显著增强,大大提高了解释的可靠程度。总之,共反射面元叠加技术在莺—琼盆地具有较高的推广应用价值。     

Metal dynamics in an Antarctic food chain

The concentrations of copper, zinc, cadmium, selenium and mercury were determined in eggs, muscle, liver, kidney and stomach content of nestilings and adults of the Antarctic petrel, Thalassoica antarctica, and its predator, the south polar skua, Chataracta maccormicki, from Svarthamaren, Dronning Maud Land, Antarctica. The dominant food of the petrels is krill, Euphausia superba. The results show relatively high levels of cadmium in krill, which is assumed to be the main reason for the high levels of cadmium in petrels and skuas. Cadmium is almost absent in eggs, but accumulates very rapidly with age in nestlings. The copper concentrations in livers of nestling petrels reach very high levels during growth. This may be seen in connection with physiological development processes. Mercury seems to be accumulated with age and between trophic levels. Among the nestlings, the mercury levels decrease with increasing age, which may be accomplished by the excretion of mercury through the growth of feathers and as a dilution effect during growth. Selenium and mercury are inversely correlated in nestlings. The levels of zinc were similar for different nestling stages and between nestlings and adults in skuas and petrels.     

Abiotic ammonium formation in the presence of Ni-Fe metals and alloys and its implications for the Hadean nitrogen cycle

Experiments with dinitrogen-, nitrite-, nitrate-containing solutions were conducted without headspace in Ti reactors (200°C), borosilicate septum bottles (70°C) and HDPE tubes (22°C) in the presence of Fe and Ni metal, awaruite (Ni₈₀Fe₂₀) and tetrataenite (Ni₅₀Fe₅₀). In general, metals used in this investigation were more reactive than alloys toward all investigated nitrogen species. Nitrite and nitrate were converted to ammonium more rapidly than dinitrogen, and the reduction process had a strong temperature dependence. We concluded from our experimental observations that Hadean submarine hydrothermal systems could have supplied significant quantities of ammonium for reactions that are generally associated with prebiotic synthesis, especially in localized environments. Several natural meteorites (octahedrites) were found to contain up to 22 ppm N_tot. While the oxidation state of N in the octahedrites was not determined, XPS analysis of metals and alloys used in the study shows that N is likely present as nitride (N^3-). This observation may have implications toward the Hadean environment, since, terrestrial (e.g., oceanic) ammonium production may have been supplemented by reduced nitrogen delivered by metal-rich meteorites. This notion is based on the fact that nitrogen dissolves into metallic melts.     

Fully-implicit simulation of vertical-equilibrium models with hysteresis and capillary fringe

Geological carbon storage represents a new and substantial challenge for the subsurface geosciences. To increase understanding and make good engineering decisions, containment processes and large-scale storage operations must be simulated in a thousand year perspective. A hierarchy of models of increasing computational complexity for analysis and simulation of large-scale CO₂ storage has been implemented as a separate module of the open-source Matlab Reservoir Simulation Toolbox (MRST). This paper describes a general family of two-scale models available in this module. The models consist of two-dimensional flow equations formulated in terms of effective quantities obtained from hydrostatic reconstructions of vertical pressure and saturation distributions. The corresponding formulation is fully implicit and is the first to give a mass-conservative treatment and include general (non-linearized) CO₂ properties. In particular, the models account for compressibility, dissolution, and hysteresis effects in the fine-scale capillary and relative permeability functions and can be used to accurately and efficiently study the combined large-scale and long-term effects of structural, residual, and solubility trapping.     

A second-order particle tracking method

An accurate particle tracking method for a finite difference method model is developed using a constant acceleration method. Being assumed constant temporal and spatial gradients, the new method permits temporal-spatial variability of particle velocity. Test results in a solid rotating flow show that the new method has second-order accuracy. The performance of the new method is compared with that of other methods; the first-order Euler forward method, and the second-order Euler predictorcorrector method. The new method is the most efficient method among the three. It is more accurate and efficient than the other two.     

黑潮和北大西洋海温异常在2008年1月我国南方雪灾中的可能作用的数值模拟

根据NCEP/NCAR再分析资料、NOAA扩展重建海表温度资料,利用中国科学院大气物理研究所全球大气环流谱模式（IAP-T42L9）模拟了大气环流对黑潮区、北大西洋海温异常偏暖的响应特征,并探讨了黑潮区、北大西洋海温异常在2008年1月我国南方雪灾中的作用。通过模拟场与观测场的比较表明,在考虑黑潮区和北大西洋海温异常偏暖的情况下,模式比较成功地模拟出了2008年1月我国南方发生低温、雨雪、冰冻天气的各种主要环流特征。黑潮区海温升高导致东亚沿海位势高度增加,西太平洋副热带高压偏北。它一方面不利于冷空气向下游输送,导致冷空气在我国长江流域及其以南地区堆积;另一方面加强了海洋的暖湿气流及向我国长江流域及其以南地区的水汽输送,为我国南方雨雪天气形成提供了充沛的水汽条件,有利于我国南方低温、雨雪、冰冻天气的持续。北大西洋海温异常偏暖对中高纬地区中西伯利亚阻塞高压的形成有重要作用。受其影响,我国除受东路冷空气活动的影响外,中纬度还不断有西路冷空气随着西风带槽脊东移影响我国东部地区。它是导致我国南方低温、雨雪、冰冻天气持续的另一个重要原因。     

Adenine oxidation by pyrite-generated hydroxyl radicals

Cellular exposure to particulate matter with concomitant formation of reactive oxygen species (ROS) and oxidization of biomolecules may lead to negative health outcomes. Evaluating the particle-induced formation of ROS and the oxidation products from reaction of ROS with biomolecules is useful for gaining a mechanistic understanding of particle-induced oxidative stress. Aqueous suspensions of pyrite particles have been shown to form hydroxyl radicals and degrade nucleic acids. Reactions between pyrite-induced hydroxyl radicals and nucleic acid bases, however, remain to be determined. Here, we compared the oxidation of adenine by Fenton-generated (i.e., ferrous iron and hydrogen peroxide) hydroxyl radicals to adenine oxidation by hydroxyl radicals generated in pyrite aqueous suspensions. Results show that adenine oxidizes in the presence of pyrite (without the addition of hydrogen peroxide) and that the rate of oxidation is dependent on the pyrite loading. Adenine oxidation was prevented by addition of either catalase or ethanol to the pyrite/adenine suspensions, which implies that hydrogen peroxide and hydroxyl radicals are causing the adenine oxidation. The adenine oxidation products, 8-oxoadenine and 2-hydroxyadenine, were the same whether hydroxyl radicals were generated by Fenton or pyrite-initiated reactions. Although nucleic acid bases are unlikely to be directly exposed to pyrite particles, the formation of ROS in the vicinity of cells may lead to oxidative stress.     

有机烃新方法在金矿床快速定位预测中的应用:以陕西八卦庙特大型金矿床为例

有机质对金属元素的迁移、沉积和富集成矿有重要的作用，有机烃是有机质最终的裂解产物，具有很强的挥发性和穿透性。研究发现，八卦庙金矿床有机烃与金矿体品位呈密切相关关系，矿床的矿化富集中心也是有机烃的浓集中心。利用有机烃的这一特点可以进行隐伏金矿床的定位预测研究。