A modified analytical method for the shipboard determination of nanomolar concentrations of orthophosphate in seawater

A method for the determination of nanomolar concentrations of orthophosphate in oligotrophic seawater developed by Liang et al. (2007) has been modified to make it fully feasible for shipboard application and for faster sample throughput with minimized sample volume. The technique is based on the flow injection method with solid phase extraction on a Sep-Pak C18 cartridge and colorimetric detector. The Schlieren effect was minimized by rinsing the cartridge sequentially with 5 mL water and 2 mL 95% ethanol solution. With three micro pumps in parallel, savings of up to 80% in amount of reagents and 25% volume of seawater samples could be achieved in comparison to the previous method. Variation of stopped flow time and sample loading time gave 3 different standard curves, which corresponded to 3 linear ranges within 3.4 and 515 nM. The modified method permits the analysis of samples over a wide range of concentrations, and has been successfully applied to shipboard determination of trace orthophosphate in more than 200 seawater samples during a one-month cruise in the South China Sea. For seawater at concentrations of 20.6, 82.5, 206.2 nM orthophosphate, the relative standard deviations (RSD) (n = 6), determined daily for 6 days on board ship were 4.45%, 4.73% and 6.75%, respectively. Five seawater samples collected in the Station SEATS (South East Asia Time Series Station at 18°N, 116°E) were analyzed using the present method both on board and in a land-based laboratory, as well as with the magnesium hydroxide-induced coprecipitation (MAGIC) method, and showed no significant difference according to the statistical t-test.     

<Superscript>234</Superscript>Th/<Superscript>238</Superscript>U disequilibrium and particulate organic carbon export in the northern South China Sea

We utilized ²³⁴Th, a naturally occurring radionuclide, to quantify the particulate organic carbon (POC) export rates in the northern South China Sea (SCS) based on data collected in July 2000 (summer), May 2001 (spring) and November 2002 (autumn). Th-234 deficit was enhanced with depth in the euphotic zone, reaching a subsurface maximum at the Chl-a maximum in most cases, as commonly observed in many oceanic regimes. Th-234 was in general in equilibrium with ²³⁸U at a depth of ∼100 m, the bottom of the euphotic zone. In this study the ²³⁴Th deficit appeared to be less significant in November than in July and May. A surface excess of ²³⁴Th relative to ²³⁸U was found in the summer over the shelf of the northern SCS, most likely due to the accumulation of suspended particles entrapped by a salinity front. Comparison of the ²³⁴Th fluxes from the upper 10 m water column between 2-D and traditional 1-D models revealed agreement within the errors of estimation, suggesting the applicability of the 1-D model to this particular shelf region. 1-D model-based ²³⁴Th fluxes were converted to POC export rates using the ratios of bottle POC to ²³⁴Th. The values ranged from 5.3 to 26.6 mmol C m⁻²d⁻¹ and were slightly higher than those in the southern SCS and other oligotrophic areas. POC export overall showed larger values in spring and summer than in autumn, the seasonality of which was, however, not significant. The highest POC export rate (26.6 mmol C m⁻²d⁻¹) appeared at the shelf break in spring (May), when Chl-a increased and the community structure changed from pico-phytoplankton (<2 μm) dominated to nano-phytoplankton (2–20 μm) and micro-phytoplankton (20–200 μm) dominated.     

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?????????????????￡????ú?????????GPS Block IIR??M??????????????????????о??????????GM(1??1)??AR(p)??????????1???????????????1 ns????????10??????????????10 ns??????????????ζ??????GM??1??1???????????????????????IGS(the International GPS Service for Geodynamics)??????????7 ns??????     

鄂尔多斯盆地塔巴庙地区上古生界源岩热演化特征

鄂尔多斯盆地北部主力气源岩太原组、山西组煤系地层热成熟史的研究对本区天然气充注过程和有利目标区预测具有重要的参考价值。在对研究区烃源岩评价和一维、二维地质建模研究的基础上。利用BasinMod盆地模拟软件对单井以及研究区内二维剖面、平面进行了煤系烃源岩热演化史模拟研究。研究结果表明：（1）该区在中三叠世进入生烃门限,中侏罗世以后,烃源岩持续埋深,早白垩世末期至最大埋深（4000m左右）,绝大多数的天然气都在这一阶段生成,早白垩世末构造抬升以后只有少量天然气生成;（2）研究区上古生界太原组和山西组煤系源岩最大累积生烃强度可达到2200×10^8m^3／km^2,对现今天然气的分布具有较强的控制作用。     

Landscape Effects of Land Consolidation Projects in Central China——A Case Study of Tianmen City,Hubei Province

The goal of land consolidation in China is still to develop agricultural production. The study of landscape effects of land consolidation projects （LCPs） faces many difficulties because of the lack of government＇s interest and data. This paper, taking Tianmen City of Hubei Province in Central China as an example, presents a methodology for analyzing landscape effects of LCPs by GIS and Fragstats3.3. It describes landscape effects with indexes of Patch Density （PD）, Largest Patch Index （LPI）, Landscape Shape Index （LSI）, Interspersion and Juxtaposition Index （IJI）, Aggregation Index （AI）, and Shannon＇s Diversity Index （SHDI）, showing more regular shape, simpler structure and less habitat diversity after LCPs. It computes ten landscape indexes of four categories of patches including Cultivated Land, Road, Water Channel, and River and Pond. The indexes show that 1） cultivated land becomes more fragmental in patch area, less irregular in patch shape and more concentrated in block; 2） the transport capacity of roads and irrigation and drainage capacity of water channels have been improved; 3） the landscape change of river and pond can be summarized as decreasing scale, more regular shape, reducing connectivity and diversity of the class. LCPs can facilitate agricultural production as well as protect cultivated land and food security. However, it is doubted that the increase of cultivated land from LCPs results from the reducing in landscape diversity of water area.     

海浪预报产品数据库管理系统开发研究

本文介绍海浪预报产品数据库管理系统，包括数据库管理系统的总体设计，硬软件的配置，数据库管理系统的主要内容和解决的关键技术。该数据库管理系统的建成，提供了我国近海和西北太平洋海浪预报的系统性资料，不仅对提高海浪预报准确率提供基础资料，也给海浪中长期预测预报方法研究和区域海洋学研究带来极大帮助。     

闽中、闽东渔场扁舵鲣的资源生物学

本文以1981～1984年4～9月累计18个月在闵中、闵东渔场开展中上层鱼类资源开发性探捕调查的材料,报道了扁舵鲣的生态分布、群体结构和生活史主要特征。鱼群先后于4～7月由南而北进入本渔场,数量分布以5月为多;栖息的最适温、盐度分别为23.5～25.5℃和33～34;渔获群体由0～V龄组成;性成熟的最小年龄为Ⅰ龄,最小叉长271～280mm;生长参数L_∞=484.44mm,W_∞=2050.76g,K=0.5109,t_0=0.3598;瞬时自然死亡率为0.8937,开发比率为0.2138,属于轻度利用的鱼类资源,尚有较大的开发潜力。     

全球不同构造环境蛇纹岩全岩和矿物微量元素组成及非传统稳定同位素(Fe-Zn-Cu同位素)特征

蛇纹岩对地球深部和浅部的元素循环以及氧化还原状态调节具有非常重要的作用。蛇纹岩中的流体活动性元素(fluid-mobile element, FME)是揭示地幔岩石水化、脱水以及元素循环的关键。本文系统收集和分析了前人报道的不同构造环境的蛇纹岩矿物化学、全岩微量元素和非传统稳定同位素(Fe、Zn、Cu)的组成特征,试图从多个角度总结蛇纹岩脱水过程的元素迁移规律及流体性质。蛇纹岩主要矿物蛇纹石微量元素含量具有以下主要特征：(1)不同变质程度的蛇纹岩中的蛇纹石既包含轻稀土元素(light rare earth element, LREE)富集,又包含LREE亏损的特征;(2)纤蛇纹石的REE和微量元素分布在利蛇纹石和叶蛇纹石的范围内,利蛇纹石重稀土元素(heavy rare earth element, HREE)整体上略高于叶蛇纹石且更加富集FME;(3)通过中度不相容元素与REE含量相结合,能够较好地区分橄榄石和辉石蛇纹石化所形成的蛇纹石,即辉石形成的蛇纹石富集相容元素(如Sc、Zn、Cr、Y和Ti等)并具有较高的HREE,而橄榄石形成的蛇纹石则表现为平坦且整体较低的REE分布型式。在...     

强光照和黑暗条件下刺参体壁的基因表达差异

参对光照变化非常敏感,研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况,通过“对照”与“黑暗”,“对照”与“强光”和“黑暗”与“强光”的比较,在|log₂ ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明,“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多,而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对,发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。     

Differential gene expression in the body wall of the sea cucumber(Apostichopus japonicus) under strong lighting and dark conditions

Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light("light"), normal light("control") and fully dark("dark") environment. In the comparisons of "control" vs. "dark", "control" vs. "light" and "dark" vs."light", 1 161, 113 and 1 705 differentially expressed genes(DEGs) were identified following the criteria of|log_2 ratio|≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that "cellular process" and "binding"enriched the most DEGs in the category of "biological process" and "molecular function", while "cell" and "cell part" enriched the most DEGs in the category of "cellular component". And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.