The Japanese medaka (Oryzias latipes) model: applicability for investigating the immunosuppressive effects of the aquatic pollutant benzo[a]pyrene (BaP)

Despite the fact that BaP is a carcinogen, mammalian immunosuppressant, and ubiquitous aquatic pollutant, knowledge regarding the effects of BaP on the immune system of fish is still lacking. To begin to fill this gap, studies were conducted in medaka to examine the effects and mechanisms by which BaP exposure might alter host immunocompetence. Fish, exposed by IP injection of BaP (2-600 microg/g BW), were examined after 48 h for effects upon immune function and CYP1A expression/activity. Benzo[a]pyrene, at a concentration below that which increased levels of CYPIA expression/activity (2 microg BaP/g BW) suppressed lymphocyte proliferation. Concentrations of BaP at 20 and 200 microg/g BW. suppressed antibody-forming cell (AFC) numbers, superoxide production, and host resistance against bacteria. In contrast, exposure to the low affinity aryl hydrocarbon receptor (AhR) agonist, benzo[e]pyrene (BeP), neither induced CYP1A expression nor altered immune function. Given the lack of immunosuppressive effects produced by BeP, and the fact that exposure to the AhR antagonist (and CYP1A inhibitor) alpha-naphthoflavone (ANF) ameliorated the suppressive effects of BaP upon AFC numbers, the AhR pathway (including CYP1A-mediated production of reactive BaP metabolites) appears important in mediating BaP-induced immunotoxicity in fish, as in mammals. In the past, the medaka has proven a successful model for assessing carcinogenic agents. These studies have demonstrated its utility for also determining the immunosuppressive effects of an important aquatic contaminant.     

中华绒螯蟹人工半咸水工厂化育苗试验研究报告

在盐度为14—19‰的人工海水中,温度20—25℃条件下,189只平均体重100g的抱卵亲蟹产蚤状幼体4025万只。布苗密度为50—100万只/m~3为宜。必须经常换水,保持水质良好。人工饵料以单胞藻和丰年虫无节幼体为主,可适当加喂蛋黄。     

Wave interaction with a perforated wall breakwater with a submerged horizontal porous plate

This study examines the hydrodynamic performance of a new perforated-wall breakwater. The breakwater consists of a perforated front wall, a solid back wall and a submerged horizontal porous plate installed between them. The horizontal porous plate enhances the stability and wave-absorbing capacity of the structure. An analytical solution based on linear potential theory is developed for the interaction of water waves with the new proposed breakwater. According to the division of the structure, the whole fluid domain is divided into three sub-domains, and the velocity potential in each domain is obtained using the matched eigenfunction method. Then the reflection coefficient and the wave forces and moments on the perforated front wall and the submerged horizontal porous plate are calculated. The numerical results obtained for limiting cases are exactly the same as previous predictions for a perforated-wall breakwater with a submerged horizontal solid plate [Yip, T.L., Chwang, A.T., 2000. Perforated wall breakwater with internal horiontal plate. Journal of Engineering Mechanics ASCE 126 (5), 533–538] and a vertical wall with a submerged horizontal porous plate [Wu, J.H., Wan, Z.P., Fang, Y., 1998. Wave reflection by a vertical wall with a horizontal submerged porous plate. Ocean Engineering 25 (9), 767–779]. Numerical results show that with suitable geometric porosity of the front wall and horizontal plate, the reflection coefficient will be always rather small if the relative wave absorbing chamber width (distance between the front and back walls versus incident wavelength) exceeds a certain small value. In addition, the wave force and moment on the horizontal plate decrease significantly with the increase of the plate porosity.     

台湾海峡台风暴潮非线性数值计算

台湾海峡平均水深不大于50米,台风侵袭频繁.由于台湾岛的“墙壁效应”和台湾海峡的“狭管效应”的影响,台湾海峡的台风暴潮显得较为独特.以前,我们及其他兄弟单位对福建沿海台风暴潮的研究,大多数仅局限于单站的气-水动力相互作用的研究,很少涉及整个海峡.虽说我们也曾作过福建沿岸台风暴潮现场调查,但进行次数有限,了解的也仅是海峡西岸局部地区的情况,它仅能反映台湾海峡台风暴潮的一个侧面,而无法了解整个海峡地区的风暴潮全貌.     

SAS测量水体表观光学参数的方法

SeaWiFS航空模拟器（SAS）是专门为在水面之上测量水体表观光学参数的现场设备。文章在介绍SAS现场测量以及数据处理基本方法的基础上，分析了2002年黄海试验SAS的应用效果，并与剖面法测量数据进行了比对。     

菲律宾蛤仔(Ruditapes philippinarum)蛋白聚糖的分离提取及其抗肿瘤活性的初步研究

采用凝胶过滤层析、SDS-PAGE电泳、Bradford法和苯酚-硫酸法等生物化学方法对菲律宾蛤仔中的蛋白聚糖(PG1和PG2)进行分离纯化,研究其理化性质。采用MTT方法研究蛋白聚糖对人肝癌细胞SMMC-7721和人正常肝细胞HL-7702生长的影响。通过小鼠体内实验研究蛋白聚糖的抗肿瘤活性和对荷瘤小鼠免疫功能的影响。结果表明,PG1分子量大约为20kDa,多糖含量为70.09%,蛋白质含量为15.4%。PG2的多糖含量为82.53%,蛋白质含量为7.26%。PG1在200μg/ml浓度时对SMMC-7721的生长抑制率为73.30%,随浓度的升高,抑制率并未发生明显改变。PG2对SMMC-7721的生长抑制作用呈剂量依赖性,在500μg/ml的浓度下,其抑制效果与PG1的抑制效果相当。在同样浓度下,PG1对人正常肝细胞的生长和功能没有明显影响。PG1呈剂量-效应关系明显增加荷瘤小鼠脾指数,促进荷瘤小鼠脾淋巴细胞转化,且没有降低体重及其他毒副作用。PG1对小鼠S180肉瘤的抑制作用可能是通过直接杀伤肿瘤细胞的细胞毒作用和提高小鼠细胞免疫水平来实现。     

浅述多元数据处理的一种简化拟合形式及算法剖析

在实际工程试验与研究中 ,多元数据处理经常遇到 ,根据 Weierstrass定理 ,采用多项式拟合是一种常见的数据处理方法。对于多元多项式的系数求解问题 ,可以归结为超定方程组的求解问题。该文以超定方程组的 SVD求解算法为核心 ,阐述了在工程实际中关于多元数据处理的一种应用方法 ,并尝试给出多元多项式拟合的一种简化方法     

Multi-scale variability of beach profiles at Duck: A wavelet analysis

Beach profiles have been observed to change over a range of spatial and temporal scales; however techniques for quantifying this variability have not been fully established. In this paper, a wavelet technique is introduced as a method to study the multi-scale variability of beach profiles. The beach profile data comprising a 22-year time series surveyed at the US Army Corps of Civil Engineers Field Research Facility (FRF) at Duck are analysed using the adapted maximal overlap discrete wavelet transform (AMODWT). The analysis successfully identifies strong local features in the variability of beach profiles in time and space separately that cannot be isolated by traditional statistical methods. The analysis of spatial wavelet variances provides a new means of investigating the depth of closure. Analysis of variances by temporal scales shows that the combined effects of several temporal scales with one or two dominant scales can be seen at particular points across profiles whilst the dominant temporal scales are different at different portions of the profiles. The method allows for the extremely nonstationary behaviour of beach profile to be analysed into separate frequency bands that can facilitate the interpretation of morphological changes in terms of physical processes.     

Notes The Ocean Drilling Program (ODP)

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A Preliminary Study of Shear Wave in Seafloor Surface Sediments

This article preliminarily reports and analyses the transmission characteristics and behaviors of shear wave in the offshore seafloor surface sediments in China, discusses the relationships between the physical and mechanical features of the shear wave and the compression wave, and compares the testing results with that of Hamilton and Chen et al. The result shows that the shear wave can be tested if the seafloor surface sediment has tangent modulus. The shear wave velocity ranges from 50-600 m/s and the measuring frequency from 50-200 kHz. The sound velocity rate of shear wave and compression wave can be used to appraise the stress-strain feature of seafloor surface sediments. This study provides a basis for further describing and appraising the seafloor sedimentary acoustic-mechanical feature and building a geological-acoustic model on China's offshore sea area.