Investigating pesticide transport in the León-Chinandega aquifer, Nicaragua

Nicaragua’s León-Chinandega aquifer has seen extensive contamination by persistent organochlorine pesticides applied over decades of intensive agricultural activity. Models of flow and transport of a 330 km² sub-region of the aquifer were developed to test conceptual models of contaminant transport, to constrain the value of certain key transport parameters, and to investigate contamination-related concerns raised in past studies. To support these models, a variety of hydrogeologic and geochemical data were collected. It was concluded that the organochlorine pesticides seen in groundwater originate in soils, and are transported to the water table through widespread preferential flow, through shortcutting around wells, or through wind-blown particles delivered to poorly protected hand-dug wells. The distribution coefficient (K _d) of these pesticides is estimated to be between 0.1 and 100 ml/g and the concentration of pesticides being delivered to the water table is estimated to be between 10² and 10⁵ ng/L. It was found that the distribution and concentration of pesticides in the aquifer would be affected by an increase in groundwater abstraction within the region.

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Résumé L’aquifère du León-Chinandega au Nicaragua a été soumis à des contaminations étendues par des pesticides organochlorés persistants qui ont été répandus pendant des dizaines d’années d’agriculture intensive. Des modèles d’écoulement et de transport d’une sous-région de 330 km² de l’aquifère ont été construits pour tester des modèles conceptuels du transport de contaminants, pour contraindre la valeur de certains paramètres de transport clefs, et pour étudier les problèmes liés au transport de contaminations qui avaient été rencontrés lors d’études précédentes. Différents types de données hydrogéologiques et géochimiques ont été collectés pour réaliser ces modèles. La conclusion montre que les pesticides organochlorés rencontrés dans les eaux souterraines proviennent des sols et qu’ils sont transportés jusqu’à l’aquifère via de grandes zones d’écoulement préférentiel, via des raccourcis hydrauliques autour des puits, ou encore via des particules emportées par le vent et tombées dans des puits creusés à la main et mal protégés. Le coefficient de distribution (K _d) de ces pesticides est estimé entre 0.1 et 100 ml/g et la concentration de pesticides arrivant dans la nappe est estimée entre 10² et 10⁵ ng/L. L’étude a montré qu’une augmentation des prélèvements d’eau souterraine dans la région aurait un impact sur la distribution et la concentration des pesticides dans l’aquifère.

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Resumen El acuifero León-Chinandega (Nicaragua) presenta una contaminación extensiva por la aplicación de plaguicidas organoclorados en la agricultura intensiva durante décadas. Se han desarrollado modelos de flujo y transporte de una subregión del acuífero de 330 km² para comprobar los modelos conceptuales del transporte de contaminantes, estimar los valores de ciertos parámetros clave de transporte e investigar los temas relacionados con la contaminación en estudios anteriores. Para elaborar estos modelos, se recogió una variedad de datos hidrogeológicos y geoquímicos. Se ha concluido que los plaguicidas organoclorados presentes en el agua subterránea se originan en el suelo y son transportados hacia el nivel freático a través de flujos preferentes generalizados, a través de cortes alrededor de los pozos o a través de partículas transportadas por el viento depositadas en pozos excavados a mano con poca protección. El coeficiente de distribución (K _d) de estos plaguicidas se ha estimado entre 0.1 y 100 ml/g y la concentración de plaguicidas que sale hacia el nivel freático se estima que puede estar entre 10² y 10⁵ ng/L. Se ha concluido que el incremento de la extracción de aguas subterráneas dentro de la región afectaría la distribución y concentración de plaguicidas en el acuífero.

     

Tidal and Nontidal Oscillations in Elkhorn Slough,CA

Elkhorn Slough is a shallow, tidally forced estuary that is directly connected to Monterey Bay. It is ebb-dominated and, due to continued erosion, the tidal prism has tripled over the past 40 years. Water level measurements at four locations are used to examine tidal and nontidal oscillations in Elkhorn Slough. The tidal response of Elkhorn Slough differs from that of Monterey Bay primarily due to the generation of a relatively large number of shallow-water tidal constituents that are due to tidal distortion caused by friction along the bottom and lateral boundaries, intertidal storage, and nonlinear advection. The shallow-water constituents range from 3 to almost 15 cycles per day (cpd) and include a rich variety of overtides and compound tides, whose amplitudes generally increase toward the head of the slough. The tidal harmonics are seasonally dependent, with lower amplitudes during the fall and winter and higher amplitudes in summer. The tidal constituents were examined using two types of spectral decomposition, the conventional power spectrum and the more recent Hilbert spectrum. Unlike the power spectrum, the Hilbert spectrum does not reveal any harmonic structure in the data. Energy associated with tidal distortion in this case appears to be broadly distributed across the spectral continuum. At least four nontidal oscillations occur in Elkhorn Slough with frequencies of 26.0, 39.7, 52.7, and 66.9 cpd. The Hilbert spectrum reveals maxima at 26, 39.7, and 66.9 cpd, but not at 52.7 cpd, suggesting that it is harmonically related to the oscillation at 26.0 cpd. The nontidal oscillations fall into the range of frequencies associated with the natural oscillations of Monterey Bay. However, evolutionary power spectra indicate that they appear to be permanent features of the system and thus are not necessarily consistent with seiche-like oscillations that are often transient and subject to damping. These oscillations could be caused by several factors including edge waves along the coast of Monterey Bay, long-period surface waves of atmospheric origin that enter the bay from offshore, or breaking internal waves in and around the Monterey Submarine Canyon. In conclusion, detailed hydrodynamic models are needed to provide a better understanding of how tidal harmonics are generated and preserved in Elkhorn Slough, and to determine the origin of the natural oscillations in Monterey Bay.     

Determination of phase transition pressures of ZnTe under quasihydrostatic conditions

Pressure behavior of ZnTe at room temperature was studied using an X-ray energy dispersive method on a DIA type cubic anvil apparatus (SAM-85) at NSLS-X17B1. By using powdered polyethylene, the sample and NaCl for a pressure scale were held under quasihydrostatic conditions, which were confirmed by X-ray diffraction method. Two high-pressure phase transitions were confirmed using X-ray powder diffraction simultaneously with electrical resistance measurements. The phase transition pressures under quasihydrostatic conditions were determined to be 9.6 GPa, at which the resistance increased, and 12.0 GPa, which was the midpoint of a large resistance decrease. Errors in the pressure determinations were estimated to be less than 0.2 GPa. These pressure values may depend on grain size and anisotropic stress effects on the calibrant. From X-ray observation of ZnTe, the bulk modulus of the zinc blende structure was calculated to beK ₀=51(3) GPa andK ₀ =3.6(0.8), and the first transition at 9.6 GPa was found to have about 9% volume change. It was consistent with an anomaly in the pressure generating curves.     

Io's sodium emission cloud

Strong evidence that Io's sodium emission is due to resonant scattering is given by our observations which show a monotonic increase of emission intensity with residual solar intensity. In addition we detected no emission during three eclipse observations of Io. We propose a resonant scattering model with two spacial components comprising an optically thick atmosphere extending 10³ km above Io's surface surrounded by an optically thin cloud which forms a partial torus around Jupiter. In this model a flux of 10⁷ cm^?2 sec^?1 sodium atoms are sputtered from Io's surface by heavy energetic ions which are accelerated in a plasma sheath around Io. The atoms sputtered from the surface collide with atoms in Io's atmosphere so the equipartition of kinetic energy is established. The total sodium abundance is about 3 × 10¹³ cm^?2. During Io's day, sodium and other atmospheric constituents are ionized, giving rise to the ionosphere observed by Pioneer 10. Atoms escape by means of Jeans escape from the critical level, which is at the top of the atmosphere and the base of the cloud. We have observed sodium emission 6arcsec (6 Io diameters) above and below Io's orbital plane and 23arcsec toward Jupiter in Io's orbital plane. No emission was detected at maximum elongation 180° from Io. We interpret these results to mean that atoms escaping from Io form a partial torus whose thickness is about 12 arcsec and whose length is at least one-fifth of Io's orbital circumference.     

Temporal Variations of Benthic Bacterial Microflora on the Northwestern Mediterranean Continental Shelf and Slope

Abstract. Bacterial (direct and heterotrophic counts, heterotrophic potentials) and organic matter (org C, org N) parameters were studied over 2 years in the surface sediments of 3 stations located on the continental shelf (station A: 15m; station B: 35m) and slope (station C: 910m) in the northwestern Mediterranean Basin.
Although logistic constraints did not allow a satisfactory sampling periodicity in the offshore survey, some seasonal trends were still observable at the deepest station. Variations were quantitatively more important in the two shallow sediments (from 3×10² heterotrophic bacteria – ml^-1 in December 1984 to 10⁷ heterotrophic bacteria-ml^-1 in March 1986). Epifluorcsccncc direct counts were relatively constant (5×10⁸ cells-ml^-1), while heterotrophic potentials (with ¹⁴C labelled glucose and glutamic acid) showed a succession of very short pulses over the year. Different concentrations of organic matter (from 0.2% orgC at 15m to 0.8% orgC at 35m) appeared to sustain very similar bacterial numbers. Nevertheless, a certain relation between the seasonal evolution of bacterial and organic matter parameters was discernable. Although depth apparently had no measurable effect on maximal spring bacterial densities, it seemed to lessen the summer and winter decreases in the two shallow stations.     

Assessment of putative endocrine disrupters in an in vivo crustacean assay and an in vitro insect assay

Concern over endocrine disrupters in coastal ecosystems has stimulated global efforts to understand their potential impacts on fish and invertebrate communities. Given that marine crustaceans are sensitive to the effects of alkylphenols and other xenobiotics, we are currently investigating whether these responses may be caused via an endocrine mechanism. Tisbe battagliai (Copepoda: Harpacticoida) is representative of an ecologically important group of meiofauna, is sensitive to toxicants and is used as an international test species in marine ecotoxicology. A 21-day life-cycle test that incorporates assessment of survival, development, reproduction and sex ratios, has shown that populations of T. battagliai are not significantly affected by environmentally relevant levels of steroidal oestrogen agonists, or by related synthetic receptor agonists. The absence of in vivo effects due to these steroid agonists and antagonists prompted in vitro studies of ecdysteroid receptor activity of a range of reference compounds and environmental contaminants with an ecdysteroid receptor (EcR/USP)-based screening assay derived from the BII haemocyte line of Drosophila melanogaster. The implications for environmental monitoring of endocrine disrupters are discussed.     

Partial purification and properties of cytochrome P450 from digestive gland microsomes of the common mussel, Mytilus edulis L.

Cytochrome P450 from the digestive gland of M. edulis was partially purified by sodium cholate solubilization, 4–15% polyethylene glycol fractionation, and octyl-Sepharose affinity, DEAE-Sephacel ion-exchange and hydroxylapatite chromatography (yields of up to 7–10%). Three peaks were resolved by DEAE-Sephacel chromatography (termed peaks 1–3). P450 specific content was increased from 26 to 800 pmol per mg protein, and the ratio of P450 content to NADPH-cytochrome c (P450) reductase activity reduced by a factor of 250. Oxidised spectrum λmax of P450 was 410.5 ± 1.5 nm. Type II difference spectra were seen with both type II (clotrimazole, metyrapone) and type I (α-naphthoflavone, 7-ethoxycoumarin) compounds. Western blotting with polyclonal anti-P4501A from perch (Perca fluviatilis) gave a single band of approximately 54 kDa molecular weight. A reconstituted system containing peak 2 or 3, rat liver P450 reductase, lipid and NADPH metabolised benzo[a]pyrene to diones, diols, phenols and putative protein adducts. Peak 2 plus cumene hydroperoxide was indicated to produce diones and protein adducts only. Peak 2 alone was indicated to produce diones and phenols. The major free metabolites in all cases were diones (53–100%). The results indicate the existence of a P4501A-like enzyme in M. edulis, possibly with unusual properties as indicated by the difference spectra, metabolism in absence of NADPH and added P450 reductase, and predominance of diones.