温度对刺参繁殖期消化酶和代谢酶活力的影响

为探究刺参(Apostichopus japonicus)繁殖期消化、代谢生理特点,作者以刺参亲参(240 g±15 g)为实验材料,设置了5个温度水平(6、10、14、18、21℃),研究了温度对刺参繁殖期内肠道消化酶和体壁肌肉代谢酶活力的影响。结果发现:温度对亲参繁殖期消化酶、代谢酶活力受培育水温影响显著(P0.05)。其中,胰蛋白酶与淀粉酶活力随水温升高呈先升高后降低的趋势,在10℃时活力最高且达到峰值;脂肪酶活力随着养殖水温升高呈下降趋势,在6℃时活力最高。己糖激酶、丙酮酸激酶、乳酸脱氢酶、苹果酸脱氢酶活力随水温升高出现先增高后降低的趋势,在10℃时达到峰值;而琥珀酸脱氢酶活力随温度升高呈下降趋势。研究表明,10~14℃温度下,亲参消化酶与代谢酶活力均处于较高水平,是理想的亲参室内促熟温度参数。     

Distribution of ammonia-oxidizing Betaproteobacteria community in surface sediment off the Changjiang River Estuary in summer

The spatial distribution of ammonia-oxidizing Betaproteobacteria ( β AOB) was investigated by FISH (fluorescence in situ hybridization) and DGGE (denaturing gradient gel electrophoresis) techniques in the sediment off the Changjiang River Estuary.Sediment samples were collected from eight stations in June before the formation of hypoxia zone in 2006.The abundance of β AOB ranged from 1.87 × 10 5 to 3.53 × 10 5 cells/g of sediment.β AOB abundance did not present a negative correlation with salinity,whereas salinity was implicated as the primary factor affecting nitrification rates.The DGGE profiles of PCR-amplified amo A gene fragments revealed that the β AOB community structure of sample S2 separated from other samples at the level of 40% similarity.The variations in composition of β AOB were significantly correlated with the salinity,temperature,absorption ability of sediments and TOC. The statistical analysis indicates that the β AOB abundance was a main factor to influence nitrification rates with an influence ratio of 87.7% at the level of 40% biodiversity similarity.Considering the good correlation between β AOB abundance and nitrification estimates,the abundance and diversity of β AOB community could be expected as an indirect index of nitrification activity at the study sea area in summer.     

南黄海中部沉积物物理性质与压缩波速相关性分析

根据南黄海中部海底沉积物柱状样的实验室土工试验测试结果,分析沉积物的物理特征,并通过实验室方法获得沉积物压缩波速,探讨物理参数与压缩波速的相关关系,得到经验公式。结果表明:该区域沉积物以淤泥和淤泥质黏土为主,具有含水率高、孔隙比大、塑限高等特征;物理参数与压缩波速之间有较好的相关关系,密度、孔隙比、含水率、塑限、液限及塑性指数与压缩波速的相关性最明显,其中,密度与压缩波速成正比相关关系,而孔隙比、含水率、塑限、液限和塑性指数与压缩波速成反比相关关系;以沉积物天然密度和塑性指数为自变量的双变量回归分析方程能更好地表达沉积物物理特性与压缩波速的相关关系。     

中国九龙江河口营养盐基准值研究

One of the key concerns in estuarine and coastal environments is eutrophication, which is known to be closely connected to nutrient enrichment. To control and improve surface water quality, nutrient criteria are recommended in order to provide reference conditions for the environment. However, the current datasets of nutrient criteria in China are either designed for surface water or sea water, and as such these are not suitable for assessing estuarine water quality, especially given the unique ecosystem and dynamic properties of estuaries. To address this issue in current estuarine water management, nutrient criteria have been developed for a local estuary in the southeast of China. Segmentation of the Jiulong River Estuary, China was firstly analysed through field investigations carried out during 2013–2014, which resulted in the delineation of three segments characterized by their distinct properties in relation to salinity, hydrology, ecosystems, etc. Historical records from 1997 to 2014 were then analysed using statistical modelling to develop candidate nutrient criteria for the estuary.This stage of the analysis was undertaken in combination with the study of nutrient status records from the 1980s,which were used to establish a baseline reference condition. The recommended criteria values of dissolved inorganic nitrogen(DIN) in Segments Ⅰ, Ⅱ and Ⅲ of the Jiulong River Estuary are 64, 21 and 14 μmol/L,respectively, and the corresponding values for soluble reactive phosphorus(SRP) are 0.89, 0.76 and 0.89 μmol/L,respectively. It should be emphasised that these values are site-dependent, and that different results may be achieved at other locations depending on the physical and biogeochemical characteristics of an estuary, or even a single site within a catchment. It is hoped that by demonstrating a possible methodological approach and methods of nutrient criteria derivation in the Jiulong River Estuary, the current study will offer researchers some fundamental basis from which to begin to develop more complete nutrient criteria indices for the study of nutrient conditions in other estuaries throughout China.     

鲫鱼(Carassius auratus)半胱亚磺酸脱酸酶CSAD基因克隆及其表达研究

本研究以鲫鱼(Carassius auratus)为研究对象,采用巢式PCR和RACE技术克隆鲫鱼半胱亚磺酸脱酸酶CSAD基因c DNA序列,并且利用实时荧光定量PCR检测了CSAD m RNA在鲫鱼不同组织和昼夜节律中的相对表达水平,同时还研究了牛磺酸对鲫鱼肠道CSAD m RNA表达的影响。结果表明:(1)鲫鱼CSAD基因c DNA序列包含186bp的5?UTR序列,675bp的3?UTR序列,1503bp开放阅读框,编码500个氨基酸。同源性分析表明,鲫鱼和鲤鱼的同源性为97.2%。系统发育分析表明,鲫鱼与鲤鱼之间的亲缘关系最接近,置信度为100。经预测,其编码的蛋白质的分子量和等电点分别为56.82k Da和5.77;(2)Ca CSAD m RNA在肌肉、心脏、肠道及肝脏中的表达水平较高,在脑和鳃组织中的相对表达量较低;(3)鲫鱼肠道CSAD m RNA的相对表达量在6:00am点时最高,9:00pm点时相对表达量最低;(4)鲫鱼CSAD的相对表达丰度随着牛磺酸添加量的增加而逐渐下降。本研究结果不仅有助于理解鲫鱼CSAD基因的分子特征,同时将为进一步研究鱼类CSAD营养调控功能提供理论依据。     

椭圆度-凹坑双缺陷海底管道局部屈曲特性研究

海底管道在制造、埋设以及使用过程中极易产生椭圆度-凹坑双缺陷,双缺陷影响管道局部屈曲,对含椭圆度-凹坑双缺陷海底管道的局部屈曲特性研究十分必要。现行规范中采用等效椭圆度对含椭圆度-凹坑海底管道进行评估,该方法无法准确评估不同缺陷形式的屈曲特性。采用形状系数对含椭圆度-凹坑双缺陷的海底管道进行评估,运用有限元软件ABAQUS进行数值模拟,并进行试验验证。在此基础上对含有不同凹坑深度、不同椭圆度的海底管道进行局部屈曲的数值模拟,计算不同形状椭圆度、含有不同凹坑深度海底管道的形状系数,对其进行敏感性分析。计算结果表明:形状系数对海底管道椭圆度、凹坑深度、径厚比敏感性较强;对凹坑宽度敏感性较弱。     

一种酪氨酸酶基因在笠贝初生壳形成中的表达分析

软体动物初生壳形成是贝壳早期发育研究的重要内容。目前,初生壳形成的机制尚不清晰。本文从笠贝(Lottia goshimai)中克隆了一个酪氨酸酶基因(lgo-tyr1),聚类分析表明其与长牡蛎基因cgi-tyr1是直系同源基因。Lgo-TYR1的序列分析显示它具有两个典型的铜离子结合结构域和一个信号肽。通过整装原位杂交和电子扫描显微镜观察,检测到lgo-tyr1在担轮幼虫的圆形贝壳区以及紧邻的环状细胞带中表达,表明lgo-tyr1可能参与了担轮幼虫初生贝壳的生物合成以及贝壳区边界的确立。     

现行黄河三角洲叶瓣蚀积演化对动力环境的影响

现行黄河三角洲地貌演化已使河口动力环境发生了显著变化,但黄河改道清8汊后,现行河口以及废弃河道清水沟河口动力环境如何变化尚不清楚。基于1976、1995和2002年黄河三角洲实测水深数据,应用Delft3D模型对清水沟流路不同时期冬夏季河口动力环境进行了数值模拟,定量研究了黄河三角洲地貌演化对动力环境的影响。模拟结果表明,黄河现行三角洲地貌演化导致河口动力环境发生了显著变化,1976年河口动力环境弱,随着河嘴向海突出,1995年河口处涨、落潮最大流速较1976年增长了约60%~90%,潮致剪切力增大约2倍,冬季大风条件下浪致剪切力较1976年增加1个数量级,2002年黄河改道清8汊入海后,现行河口处涨、落潮最大流速较1995年减小约20%,潮致剪切力减小1/3,冬季大风条件下浪致剪切力是1995年的1~2倍,而废弃河道河口处涨、落潮最大流速较1995年减小约20%~40%,潮致剪切力减小约20%。黄河三角洲动力环境变化也显著影响了入海泥沙的搬运与沉积,造成清水沟河口呈“南肥北瘦”形态,且废弃后侵蚀速率不断降低。同时,地貌形态与动力环境之间的耦合关系对黄河三角洲冲淤模式的转变有重要影响。     

庙岛群岛海域沉积动力环境分区及沉积物输运趋势

基于庙岛群岛海域的107个表层沉积物样品粒度分析结果,利用Pejrup三角图、Gao-Collins粒径趋势分析等方法,对庙岛群岛海域沉积动力环境进行分区,并探讨了沉积物的输运趋势。结果表明,研究区沉积动力环境可划分为5个分区(A、CⅢ、CⅣ、DⅢ、DⅣ),以庙岛海峡为中心,各分区基本呈左右对称分布,与潮流流速中部辐聚、两侧辐散的分布特征相对应,研究区沉积物分布及沉积动力格局主要受潮流控制。研究区内存在两个沉积物输运趋势的汇聚中心,沉积物的长期输运趋势在海峡区主要受控于往复潮流,波流共同作用在外海区影响较为显著,使其具有与海峡区截然不同的特点。     

光照强度和光色对番红砗磲(Tridacna crocea)氨氮、活性磷酸盐及氧代谢的影响

采用室内实验生态学方法,以水体中氨氮、活性磷酸盐和溶氧变化为指标研究了光照强度和光色对番红砗磲代谢的影响。结果表明:(1)在0—10000lx光照时,番红砗磲排氨量逐渐减少,在光照12000lx时转为吸收海水中氨氮,并在16000lx时达到最高[1.89μg/(ind·h)];在2000lx光照条件下,番红砗磲可吸收海水中活性磷酸盐并释放氧气。其活性磷酸盐吸收量随光照强度增强而增加,在16000lx时达到最高峰。在实验光照范围内,番红砗磲产氧率随光照强度的增强逐渐增加。上述结果显示番红砗磲最佳光照强度约为16000lx。(2)当番红砗磲自黑暗转移至各光照组后,其氨氮、活性磷酸盐吸收率均随时间逐渐降低,而产氧率则逐渐升高。(3)光色显著影响番红砗磲合成代谢,蓝光最佳,红光次之,白光最差。