Dinoflagellate cysts in recent marine sediment from Guangxi, China

Total of 33 species of dinoflagellate cysts were discovered from surface sediment in the searegion of Guangxi, among them 12 cyst types (Diplopsalopsis sp.1, D. sp. 2, D. sp. 3, Cochlodinium sp., Protoperidinium sp. 1, P. sp. 2, P. compressum , Scrippsiella sp. 1, S. sp. 2, Alexandrium sp. 1, A. sp. 2, A. sp. 3) were first reported from the South China Sea. And one cyst type (Cochlodinium sp.) was first reported in the world. Scrippsiella trochoidea is the dominant species in this area, accounting for 45% of all the cysts. There are 2 cysts of toxic dinoflagellate (Alexandrium tamarensis and Gymnodinium catenatum). But there is no relationship between cyst number and grain size distribution.     

渐新世/中新世分界的地层学事件

介绍渐新世/中新世分界面(即古近纪/新近纪分界面)附近的浮游有孔虫和钙质超微化石事件及其新年龄。2004年国际地层年代表确定该分界面位于第58长偏心率周期弱振幅处,古地磁事件C6Cn.2n底面,经天文调谐后的年龄为23.03 Ma。发生在该界面附近的生物地层学事件主要是浮游有孔虫Paragloborotalia kugleri的始现面(22.96 Ma)和钙质超微化石Sphenolithus delphix的末现面(23.11 Ma)。在南海北部东沙群岛附近的大洋钻探ODP1148站,渐新世/中新世界面以崩塌沉积物为特征,有明显沉积间断,标志南海当时有较大范围的构造运动。     

褐藻胶裂解酶产生菌Vibro sp.QY102的发酵条件优化

对海洋来源的Vibro sp.QY102的产褐藻胶裂解酶的发酵条件进行研究。结果表明,该菌株最适液体培养基成分为(w/v):0.5%褐藻酸钠;0.4%蛋白胨;0.3%KH2PO4;0.7%K2HPO4.3H2O;2%NaCl;0.01%MgSO4.7H2O,pH=6.0。按3%的接种量接入培养基,30℃150 r/min振荡培养120 h,产酶达到10.2 U/mL,为优化前的4.5倍。Mg2 是该菌株产酶所必需的,这在其他褐藻胶裂解酶生产菌株中未见报道。该菌株产酶发酵条件的研究,为褐藻胶裂解酶的大规模制备及应用奠定了基础。     

3种鲈形目鱼类精子发生早期的超微结构研究

采用透射电镜技术,研究了3种鲈形目鱼类褐菖鲉Sebastiscus marmoratus、黑鲷Sparus macrocephalus及平鲷Rhabdosargus sarba(Forskal)精子发生早期精原细胞、初级精母细胞及次级精母细胞的超显微结构变化。结果表明,3种鱼生精细胞形态结构及代谢活动变化主要体现在核的变化、线粒体数目与结构的变化及高尔基体和溶酶体的行为变化。3种鱼核的变化基本一致:细胞核由近圆形或椭圆形转变为多角形;核质逐渐浓缩;核仁从明显结构至完全消失。褐菖鲉精原细胞时期线粒体分布较多,并出现拟染色质与线粒体相粘附;而平鲷与黑鲷精原细胞时期的线粒体分布较少,无拟染色质。3种鱼高尔基体与溶酶体的变化也有相似性。黑鲷的初级精母细胞中还观察到典型的环层状的髓样小体的结构。     

栉孔扇贝(Chlamys farreri)自然群体遗传多样性的RAPD分析

利用RAPD技术对栉孔扇贝(Chlamysfarreri)的四个自然群体(大连、烟台、青岛、韩国)的遗传多样性进行了分析。12个随机引物在四个群体中共检测到123个位点,其中多态位点109个,多态位点比例为88.62%;四个群体中均未检测到各群体特有的扩增带。韩国、烟台、大连、青岛四个群体的遗传多样性指数(Shannon指数)分别为0.2557、0.2557、0.2344和0.2249,以韩国和烟台群体遗传多样性较高,其次为大连群体,青岛群体最低,但它们之间无明显差异。不同群体的遗传多样性指数的平均数Hpop为0.2427,整个栉孔扇贝自然群体的遗传多样性指数Hsp为0.2503。遗传多样性剖分显示,绝大多数(97%)的遗传多样性是由群体内不同个体间的差异造成的,只有很小部分(3%)的遗传多样性与群体间的遗传分化有关。而采用基于单倍型(等位基因)之间的进化距离的AMOVA分析得到的这两个数据则分别为99%和1%,Φ值为0.010,且P=0.05,表明群体间遗传分化明显程度位于临界值。     

The stimulation on shedding reactions of sea urchins by K+ and Li+ immersion

INTRODUCTION Sea urchin is a marine animal of economic value. The gonad of Hemicentrotus pulcherrimus A. Agassiz and Anthocidaris crassispina A. Agassiz may be offered as food and medicine, the sea urchin shell may be used as fertilizer and artware and moreover, the sea urchin eggs are important materials for the studies in embryology and molecular biology. With the increasing exportation of sea urchins in China, the natural resources are gradually decreasing. In order to satisfy people's growing needs of sea     

厦门港水动力因素低频波动特征及其机制分析

本文根据历史水文气象资料，用条件谱分析方法，分析了厦门港水动力因素低频波动特征并探讨其发生机制。结果表明，低频波动清楚地表现为水位和表层盐度的波动。厦门港水位的低频波动主要源自平潭站的水位波动，风的贡献是次要的；条件谱分析了难以描述表层盐度低频波动和九江径流波动的复杂关系。     

南极中山站石榴石变粒岩的岩石学矿物学及其变质作用研究

本文报道了南极拉丝曼丘陵“中山站”石榴石变粒岩的岩石学和矿物化学特征;确定了其矿物组合及世代演化关系,利用电子探针分析了其特征变质矿物的化学组成,最后推断东南极拉丝曼丘陵地区曾发生过麻粒岩相变质作用和混合岩化作用。     

波纹巴非蛤Paphia undulata精子发生的超微结构

本文在超微结构水平上,研究波纹巴非蛤精子发生过程中细胞形态结构的变化和细胞器的演变规律,以及成熟精子的形态结构。观察到精细胞核形态变化的特点和线粒体演变过程。尤其是精细胞变态过程中顶体的形成和中心体的移动。精子为典型的初生型。本文还讨论了双壳类成熟精子核型变化的种属间的差异。     

Effects of Irregular Wave Groups on Pile Forces

The wave forces on 1, 2 and 4 vertical circular piles under the action of several wave trains of the same wave parameters, such as Hs,Tp and Mo, but different wave group factor GF are measured in the laboratory. After comparing these forces, it may be concluded that the mean and significant wave forces are almost of no difference for the wave trains with different GF. When GF is larger, the one-tenth of the wave froce extreme is slightly increased and the maximum wave force is much larger than the ones with smaller GF, to which attention must be paid in engineering practice.