Molecular and isotopic signatures in sediments and gas hydrate of the central/southwestern Ulleung Basin: high alkalinity escape fuelled by biogenically sourced methane

Natural marine gas hydrate was discovered in Korean territorial waters during a 2007 KIGAM cruise to the central/southwestern Ulleung Basin, East Sea. The first data on the geochemical characterization of hydrate-bound water and gas are presented here for cold seep site 07GHP-10 in the central basin sector, together with analogous data for four sites (07GHP-01, 07GHP-02, 07GHP-03, and 07GHP-14) where no hydrates were detected in other cores from the central/southwestern sectors. Hydrate-bound water displayed very low concentrations of major ions (Cl⁻, SO₄²⁻, Na⁺, Mg²⁺, K⁺, and Ca²⁺), and more positive δD (15.5‰) and δ¹⁸O (2.3‰) signatures compared to seawater. Cl^– freshening and more positive isotopic values were also observed in the pore water at gas hydrate site 07GHP-10. The inferred sulfate–methane interface (SMI) was very shallow (<5 mbsf) at least at four sites, suggesting the widespread occurrence of anaerobic oxidation of methane (AOM) at shallow sediment depths, and possibly high methane flux. Around the SMI, pore water alkalinity was very high (>40 mM), but the carbon isotopic ratios of dissolved inorganic carbon (δ¹³C_DIC) did not show minimum values typical of AOM. Moreover, macroscopic authigenic carbonates were not observed at any of the core sites. This can plausibly be explained by carbon with high δ¹³C values diffusing upward from below the SMI, increasing alkalinity via deep methanogenesis and eventually escaping as alkalinity into the water column, with minor precipitation as solid phase. This contrasts, but is not inconsistent with recent reports of methane-fuelled carbonate formation at other sites in the southwestern basin sector. Methane was the main hydrocarbon component (>99.85%) of headspace, void, and hydrate-bound gases, C₁/C₂₊ ratios were at least 1,000, and δ¹³C_CH4 and δD_CH4 values were in the typical range of methane generated by microbial reduction of CO₂. This is supported by the δ¹³C_C2H6 signatures of void and hydrate-bound gases, and helps clarify some contradictory interpretations existing for the Ulleung Basin as a whole. In combination, these findings suggest that deep biogenic gas and pore waters migrate upward through pathways such as hydrofractures, and measurably influence the shallow carbon cycle. As a result, cation-adjusted alkalinity/removed sulfate diagrams cannot always serve to estimate the degree of alkalinity produced by sulfate reduction and AOM in high methane flux areas.     

Estimation of undrained shear strength for saturated clay using shear wave velocity

Undrained shear strength is a fundamental parameter for estimating the stability of soft soils. This study explores the relationship between undrained shear strength, void ratio, and shear wave velocity for saturated and normally consolidated clay specimens. The undrained shear strength void ratio-shear wave velocity relationship was correlated to empirically determined parameters of selected marine clay specimens. To verify the proposed relationship between undrained shear strength and shear wave velocity, in situ flat dilatometer tests were used for determining the undrained shear strength, and downhole tests were used to assess the shear wave velocity on a natural soil deposit at various depths. The undrained shear strength estimated from the in situ shear wave velocities was compared to the undrained shear strength obtained in the field. The results show that the inferred undrained shear strength yield similar values and follow the same trends as the in situ undrained shear strength data. This method using shear wave velocity can help to nondestructively estimate the undrained shear strength of soft soils in the field and be used in both on-shore and off-shore geotechnical engineering projects.     

Impact of estuary barrage construction on fish assemblages in the lower part of a river and the role of fishways as a passage

The construction of an estuary barrage, an instream structure in the lower reaches of a river, causes significant physical changes in water flow patterns and river morphology, and results in altered environmental conditions. Here, we examined the impact of the Geum River estuary barrage, completed in 1990, on fish assemblages by using a literature search and fresh surveys of fishways in the barrage. We found that fish assemblages upstream and downstream of the barrage were altered following its completion. After construction, more species were found in the freshwater area, with a particularly great increase in freshwater species. Conversely, estuarine and marine species were only consistently caught in the downstream salt-water area, although the number of species increased. In total, 15,829 fish from 47 species and 20 families were identified at the three types (pool and weir, rubble type, and boat passage) of fishways in the barrage. The dominant species were Chelon haematocheilus, an estuarine species, Coilia nasus, a diadromous species, and Erythroculter erythropterus, a freshwater species. The mean total length of fish (101.9 ± 76.0 mm) in the boat passage fishway was approximately 100 mm lesser than those in the pool and weir (207.2 ± 112.8 mm) and rubble type (205.8 ± 112.7 mm) fishways. The boat passage fishway was the most efficient for fish movements. The current fishway system is not sufficient for fish migration, and thus additional ways are required to improve the system such as the boat passage. Few estuarine or diadromous species were found in both freshwater and salt-water areas, but freshwater fishes that accidently moved to salt-water area actively used fishways. Therefore, fishway management in the Geum River estuary barrage has to focus on freshwater fish; however, this may need to change to a focus on migratory fishes depending on ecological life cycles of migratory fish.     

Enhanced photobiological H2 production by the addition of carbon monoxide and hydrogen cyanide in two unicellular N2-fixing cyanobacterial strains isolated from Korean coasts

Photobiological H₂ from marine cyanobacterial strains is widely accepted to be an ideal clean and renewable energy source. Using the two Korean N₂-fixing unicellular cyanobacterial strains (Cyanothece sp. KNU CB MAL-031 and Cyanothece sp. KNU CB MAL-058) and the Synechococcus sp. Miami strain BG043511 we performed flask-scale experiments to measure the effect of CO and HCN addition on photobiological H₂ production. For the test, 1, 5, 10 and 30% v/v of CO in the N₂ atmosphere was applied. Enhancement of H₂ production was remarkable at 1–5% concentration range of CO addition. At CO concentrations over 5% no further cost-effective enhancement of H₂ production was detectable, which suggests to us that 1–5% CO addition should be adopted for practical photobiological H₂ production by the cyanobacterial strains. Maximum enhancement of the photobiological H₂ production by CO additions was 2–6 times over the control flasks without CO. When 3 ppm of HCN was injected into the cell suspension of BG043511, the enhancement of hydrogen production was 50–60% of that under 5% CO. Present result implies the possible recycling of waste CO and HCN for the enhancement of the photobiological H₂ production using marine cyanobacterial strains.     

Germination rate of Phyllospadix japonicus seeds relative to storage methods and periods

To determine the optimal storage method and longest possible storage period of Phyllospadix japonicus seeds, we examined post-storage germination rates using different storage methods and periods for P. japonicus seeds harvested in Korean coastal waters. P. japonicus seeds are classified as recalcitrant seeds with an average moisture content of 45.4%. Germination rates of P. japonicus seeds stored in seawater at 4 °C, seawater at room temperature with air supply, and an aquarium with continuous seawater circulation ranged from 35.0% to 43.5%, whereas seeds stored in seawater at 30°C, a refrigerator at ?20°C, and a desiccator at room temperature did not germinate. Seeds stored at 4°C maintained germination rates of 72.5～73.0% until 30 days of storage, but showed rapidly decreasing germination rates after 60 days and no germination after 180 days. Since few studies have investigated seed storage of P. japonicus, these results will serve as useful data for seed-based P. japonicus habitat restoration.     

Metagenomic characterization of viral communities in Goseong Bay,Korea

In this study, seawater samples were collected from Goseong Bay, Korea in March 2014 and viral populations were examined by metagenomics assembly. Enrichment of marine viral particles using FeCl₃ followed by next-generation sequencing produced numerous sequences. De novo assembly and BLAST search showed that most of the obtained contigs were unknown sequences and only 0.74% of sequences were associated with known viruses. As a result, 138 viruses, including bacteriophages (87%), viruses infecting algae and others (13%) were identified. The identified 138 viruses were divided into 11 orders, 14 families, 34 genera, and 133 species. The dominant viruses were Pelagibacter phage HTVC010P and Roseobacter phage SIO1. The viruses infecting algae, including the Ostreococcus species, accounted for 9.4% of total identified viruses. In addition, we identified pathogenic herpes viruses infecting fishes and giant viruses infecting parasitic acanthamoeba species. This is a comprehensive study to reveal the viral populations in the Goseong Bay using metagenomics. The information associated with the marine viral community in Goseong Bay, Korea will be useful for comparative analysis in other marine viral communities.     

Influence of environmental disturbances and reproductive strategy on genetic diversity and differentiation of Zostera marina populations on the southern coast of Korea

The genetic diversity and differentiation of four Zostera marina populations along the southern coast of Korea were estimated using random amplified polymorphic DNA (RAPD) markers to determine the effects of natural and anthropogenic stresses and reproductive strategy on within‐population genetic diversity. The mean number of alleles and gene diversities, indicating population genetic diversity, was highest in the Z. marina population that was exposed to repeated environmental disturbances, and lowest in the most undisturbed population. The higher genetic diversity in the disturbed population was associated with a higher contribution of sexual reproduction to population persistence. This suggests that both the level of disturbances and the reproductive strategy for population persistence contributed significantly to population genetic diversity at the study sites. According to the analysis of molecular variance (AMOVA), 76% genetic variation was attributable to differences among individuals within populations. The observed genetic differentiation (F_ST = 0.241) among Z. marina populations at the study sites appeared to result from reduced meadow size, increased genetic drift, and a high incidence of asexual reproduction. Increased population genetic diversity can enhance resistance and resilience to environmental disturbances; thus, this investigation of seagrass population genetics provides valuable new insights for the conservation, management, and restoration of seagrass habitats.     

Comparisons of sea surface height variability observed by pressure-recording inverted echo sounders and satellite altimetry in the Kuroshio Extension

Satellite-measured along-track and gridded sea surface height (SSH) anomaly products from AVISO are compared with in situ SSH anomaly measurements from an array of 43 pressure-recording inverted echo sounders (PIESs) in the Kuroshio Extension. PIESs measure bottom pressure (P _bot) and round-trip acoustic travel time from the sea floor to the sea surface (τ). The P _bot and τ measurements are used to estimate, respectively, the mass-loading and steric height variations in SSH anomaly. All comparisons are made after accurate removal of tidal components from all data. Overall good correlations are found between along-track and PIES-derived SSH anomalies with mean correlation coefficient of 0.97. Comparisons between the two measurements reveal that the mass-loading component estimated from P _bot is relatively small in this geographical region. It improves regression coefficients about 5?% and decreases mean root-mean-squared (rms) differences from 7.8 to 6.4?cm. The AVISO up-to-date gridded product, which merges all available satellite measurements of Jason-1, Envisat, Geosat Follow-On, and TOPEX/Poseidon interlaced, shows better correlations and smaller rms differences than the AVISO reference gridded product, which merges only Jason-1 and Envisat. Especially, the up-to-date gridded product reveals 6.8?cm rms improvement on average at sites away from Jason-1 ground tracks. Gridded products exhibit low correlation (0.75–0.9) with PIES-derived SSH in a subregion where the SSH fluctuations have relatively high energy at periods shorter than 20?days.     

Hydrogeochemistry and environmental isotopes of ground water in Jeju volcanic island,Korea: implications for nitrate contamination

Ground water from springs and public supply wells was investigated for hydrochemistry and environmental isotopes of ³H, ¹⁸O and D in Jeju volcanic island, Korea. The wells are completed in a basaltic aquifer and the upper part of hydrovolcanic sedimentary formation. Nitrate contamination is conspicuous in the coastal area where most of the samples have nitrate concentrations well above 1 mg NO₃ N/l. Agricultural land use seems to have a strong influence on the distribution of nitrate in ground water. Comparison of stable isotopic compositions of precipitation and ground water show that ground water mostly originates from rainy season precipitation without significant secondary modification and that local recharge is dominant. ³H concentration of ground water ranged from nearly zero to 5 TU and is poorly correlated with vertical location of well screens. The occurrence of the ³H‐free, old ground water is due to the presence of low permeability layers near the boundary of the basaltic aquifer and the hydrovolcanic sedimentary formation, which significantly limits ground water flow from the upper basaltic aquifer. The old ground water exhibited background‐level nitrate concentrations despite high nitrate loadings, whereas young ground water had considerably higher nitrate concentrations. This correlation of ³H and nitrate concentration may be ascribed to the history of fertilizer use that has increased dramatically since the early 1960s in the island. This suggests that ³H can be used as a qualitative indicator for aquifer vulnerability to nitrate contamination. Copyright © 2005 John Wiley & Sons, Ltd.     

La rétromorphose du talc dans les dolomies métallifères de Boukdema (Algérie); discussion du rôle des fluides

Résumé Les échantillons étudiés proviennent du gîte stratiforme de Boukdema (Algérie), où l'on observe notamment l'association dolomite-quartz-talcsphalérite. L'étude des inclusions fluides primaires des quartz contemporains, selon toute vraisemblance, de la dissolution partielle du talc, montre que ceux-ci se sont développés dans des solutions de salinités très variables (entre 4 et 28% en équivalent pondéral NaCl). L'hypothèse d'une ébullition des solutions (par suite d'une chute brutale de pression) rend compte de ce fait inhabituel. Dans le cadre d'un tel modèle, les corrections dues à la pression sont nulles et l'on peut en déduire à partir des mesures de températures d'homogénéïsation que la croissance du quartz s'est amorcée vers 250 °C. Compte tenu de cette indication thermométrique, on discute enfin du rôle relatif des facteurs physiques et chimiques (T, f_{CO 2}, a_Mg/a_Ca) sur la stabilité du talc dans le contexte minéralogique du gîte de Boukdema.

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The samples studied were collected in the Boukdema stratabound deposit (Algeria), where the association dolomite-quartz-talc-sphalerite occurs. Cryoscopic investigations of fluid inclusions in quartz indicate a wide salinity range in mineral forming fluids (4 to 28 weight % NaCl eq). Boiling of hydrothermal fluids possibly explains this unusual characteristic. In this hypothesis thermometric data need no pressure corrections and one may conclude that quartz growth (and simultaneously talc dissolution) occurred in the 250°–200 °C range. On the basis of this last indication, the relative role of T, f_{CO 2}, a_Mg/a_Ca, on talc stability is discussed in the mineralogical environment of Boukdema.