Effects of polychlorinated biphenyls on liver function and sexual characteristics in Japanese medaka (Oryzias latipes)

The effects of polychlorinated biphenyls (PCBs) on liver function and their differences between sexes were analyzed in Japanese medaka (Oryzias latipes) exposed to PCB126 or Kanechlor-400 (KC-400) using microarray. PCB exposure induced vitellogenin 1 expression in female medaka while suppressing choriogenin genes, which suggests that the effects of PCBs on estrogen-responsive genes do not occur directly through an estrogen receptor-mediated pathway. Reduction of androgen receptor alpha expression was also observed, and the gene expression pattern in PCB-exposed males changed to become more similar to that of females. Furthermore, changes in glycolysis-related genes indicate that PCB exposure might enhance glucose production via gluconeogenesis in the liver of medaka. Taken together, our results suggest that PCBs disrupt the endocrine system, especially androgen function, and may have the potential to cause demasculinizing effects. Additionally, induction of gluconeogenesis might be a response to maintain glucose levels consumed as a result of PCB exposures.     

The first confirmed Perseid lunar impact flash

The first confirmed lunar impact flash due to a non-Leonid meteoroid is reported. The observed Perseid meteoroid impact occurred at 18^h28^m27^s on August 11, 2004 (UT). The selenographic coordinates of the lunar impact flash are 48±1° N and 72±2° E, and the flash had a visual magnitude of ca. 9.5 with duration of about 1/30 s. The mass of the impactor is estimated to have been 12 g based on a nominal model with conversion efficiency from kinetic to optical energy of 2×¹⁰⁻³. Extrapolation of a power law size-frequency distribution fitting the sub-centimeter Perseid meteoric particles to large meteoroids suggests that several flashes should have been observed at this optical efficiency. The detection of only one flash may indicate that the optical efficiency for Perseid lunar impact is much lower, or that the slope of the size distribution differs between large meteoroids and typical sub-centimeter meteoric particles.     

A simulation of continental basin margin sedimentation in response to crustal movements, eustatic sea level change, and sediment accumulation rates

As eustasy, subsidence, and sediment accumulation vary, a 2D computer-based graphical simulation generates on-lapping and off-lapping geometries of both marine and near coastal alluvial deposits, reproducing timelines within sediment-bodies at basin margins. In the simulation, deposition is expressed by creation of new surfaces above previous ones. Thicknesses of layers are reduced by both erosion and compaction while their surfaces move vertically in response to tectonic change and loading. Simulation is divided into a series of equal time steps in which sediment is deposited as an array of en-echelon columns that mark the top of the previous depositional surface. The volume of sediment deposited in each time step is expressed as a 2D cross section and is derived from two right-angle triangles (sand and shale), whose areas are a 2D expression of the quantity of sediment deposited at that time step and whose length matches the width of the offshore sediment wedge seaward of the shoreline. Each column in the array is filled by both marine sediments up to sea level, and alluvial sediments to a surface determined by an alluvial angle that is projected landward from the shore to its intersection with the previous surface. Each time the area representing the sediment column is subtracted from the triangles, the triangle heights are reduced correspondingly. This process is repeated until the triangle heights match the position of sea level above the sediment surface, at which time the remaining area of the sediment triangle is deposited seaward as a single wedge of offshore sediments. This simulation is designed to aid interpretation of stratigraphic sequences. It can be used as a complement to seismic stratigraphy or can be used alone as an inexpensive test of stratigraphic models.     

Laboratory Experiment of Plasma Flow Around Magnetic Sail

To propel a spacecraft in the direction leaving the Sun, a magnetic sail (MagSail) blocks the hypersonic solar wind plasma flow by an artificial magnetic field. In order to simulate the interaction between the solar wind and the artificially deployed magnetic field produced around a magnetic sail spacecraft, a laboratory simulator was designed and constructed inside a space chamber. As a solar wind simulator, a high-power magnetoplasmadynamic arcjet is operated in a quasisteady mode of 0.8 ms duration. It can generate a simulated solar wind that is a high-speed (above 20 km/s), high-density (10¹⁸ m⁻³) hydrogen plasma plume of ∼0.7 m in diameter. A small coil (2 cm in diameter), which is to simulate a magnetic sail spacecraft and can obtain 1.9-T magnetic field strength at its center, was immersed inside the simulated solar wind. Using these devices, the formation of a magnetic cavity (∼8 cm in radius) was observed around the coil, which indicates successful simulation of the plasma flow of a MagSail in the laboratory.     

Numerical study on the flow characteristics of tidal jets induced by a tidal-jet-generator

The flow characteristics of tidal jets induced by a Tidal-Jet Generator (TJG) are investigated using a finite-difference numerical scheme, named Navier–Stokes (NS)–Marker and Cell (MAC)-TIDE, based on the fully 3D NS equations. The TJG is an enclosed rectangular breakwater, which has vertical opening and a large enclosed volume inside. During both phases of tide, strong and uni-directional jets can be obtained locally from the inlet of the TJG, due to the water level difference between the inner and outer sides of TJG.The computed results are extensively compared with three other independently developed numerical models; 3D-ADI, DVM, and CIP-CSF. These models are based on quasi-3D, 2D depth-averaged, and fully 3D NS equations, respectively. It is seen that the present fully 3D numerical model NS–MAC-TIDE can predict the maximum intensity of inlet velocity with higher accuracy than the other numerical models when compared with the empirical function proposed from the experiments. The numerical simulations based on NS–MAC-TIDE can reproduce successfully the processes of generation, development, and dissipation of tidal jets. The effects of gap opening on the main characteristics of the tidal jet flow are assessed. Through numerical assessment, it is also clearly demonstrated that the residual time of a pollutant distributed around the front of the TJG can be decreased by significant amount due to the locally induced tidal jet. The TJG can thus utilize tidal energy for water purification in local marine environment by providing a flushing mechanism.     

Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126

Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3′,4,4′,5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 μg/g b.w./day) or KC-400 (1 μg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka.     

Alteration of gene expression profiles in the brain of Japanese medaka (Oryzias latipes) exposed to KC-400 or PCB126

Polychlorinated biphenyls (PCBs) are known as neurotoxic chemicals and possibly alter animal behavior. We previously reported that PCB-exposure induced abnormal schooling behavior in Japanese medaka (Oryzias latipes). This abnormal behavior might be caused by the functional alteration of central or terminal nervous system. To understand the mechanism(s) of behavioral change by PCB-exposure, we analyzed the gene expression profiles in the brain of medaka exposed to 3,3',4,4',5-pentachlorobiphenyl (PCB126) or a PCB mixture (Kanechlor-400: KC-400) using a cDNA microarray that we constructed. Twelve FLF-II strain medaka (six individuals per treatment) were dietary exposed to PCB126 (0.01 microg/g b.w./day) or KC-400 (1 microg/g b.w./day) for three weeks. For the control, six fish were fed a control diet. After the exposure period, fish were dissected, and the brain samples were collected. The samples from control fish were pooled and used as a common reference in the microarray experiment. Microarray data were normalized by the LOWESS method, and we screened the genes whose expression levels were altered more than 1.5-fold. Gene expression profiling showed 97 down-regulated and 379 up-regulated genes in the brain of medaka exposed to PCB126. KC-400 exposure suppressed 15 genes and induced 266 genes in medaka brain. Among these genes, the expression levels of 7 and 188 genes were commonly down- or up-regulated, respectively in both treatment groups. On the other hand, 31 gene expressions were significantly different between PCB126 and KC-400 treatment groups, and three out of 31 genes were received opposite effects. In addition, the microarray data showed that thyroid hormone-responsive genes were up-regulated by PCB-exposure, which may imply that PCBs or their metabolites mimic thyroid hormone effects in the brain of PCB-exposed medaka.