Production of the Blood Pressure Lowing Peptides from Brown Alga （ Undaria pinnatifida ）

Brown alga （ Undaria pinnatifida） was treated with alginate lyase and hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates for the angiotensin-I-converting enzyme （ACE） was measured. Four hydrolysates with potent ACE-inhibitory activity were administered singly and orally to spontaneously hypertensive rats （SHRs）. The systolic blood pressure of SHRs decreases significantly after single oral administration of the brown alga hydrolysates by pro- tease S ＇ Amano＇ （from Bacillus stearothermophilus） at the concentration of 10 （mg protein） （kg body weight）^ - 1. In the 17 weeks of feeding experiment, 7-week-old SHRs were fed standard diet supplemented with the brown alga hydrolysates for 10 weeks. In SHRs fed 1.0 and 0.1% brown alga hydrolysates, elevating of systolic bloodpressure was significantly suppressed for 7 weeks. To elucidate the active components, the brown alga hydrolysates were fractionated by 1-butanol extraction and HPLC on a reverse-phase column. Seven kinds of ACE-inhibitory peptides were isolated and identified by amino acid composition analysis, sequence analysis, and LC-MS with the results Val-Tyr, Ile-Tyr, Ala-Trp, Phe-Tyr, Val-Trp, Ile-Trp, and Leu-Trp. Each peptide was determined to have an antihypertensive effect after a single oral administration in SHRs. The brown alga hydrolysates were also confirmed to decrease the blood pressure in humans.     

Notes on non-marine bivalve Trigonioides (Trigonioides?) from the mid-Cretaceous Goshoura Group, Kyushu, Japan

Trigonioides goshourensis n. sp. and Trigonioides amakusensis Kikuchi and Tashiro occur in the late Albian Eboshi Formation of the Goshoura Group in Kyushu, Japan. These Albian species are characterized by three radial pseudocardinal teeth on the thick and wide hinge plate, and are probably ancestors of Cenomanian species of Trigonioides (Kumamotoa) with four radial pseudocardinal teeth. This chronological relation may be important for the correlation of non-marine Cretaceous strata in East Asia. In addition, the habitat of T. amakusensis is interpreted as estuarine tidal flats under brackish water conditions, although Trigonioides is generally a freshwater bivalve genus.     

Changes in tidal currents in the ariake sound due to reclamation

The Ariake Sound is a highly productive estuary located in the western part of Japan. The decline in fisheries and the frequent occurrence of red tides and hypoxia indicate a deterioration in the ecosystem of the sound. A change in tidal currents, which is one of the possible causes of the deterioration, was investigated by numerical experiments. Two major changes in the topography of the sound, which may have changed the tidal currents, are examined. These are the reclamation in the innermost part of the sound and the construction of a dyke in a subembayment called Isahaya Bay. The numerical experiments show that the reclamation caused the tidal currents to decrease by more than 10% over a large area in the innermost part of the sound. The influence of the dyke on the tidal currents is relatively local compared to the reclamation; the area where tidal currents significantly decrease is located mostly in the subembayment.     

Time variation of the X-ray spectrum and optical luminosity of SCO X-1

Results of rocket observations of SCO X-1 over the spectral range of 220 keV are presented. The observations have been performed partly in India and partly in Japan under the collaboration of the three groups. The present results are compared with results of similar observations carried out by the LRL (Lawrence Radiation Laboratory) group. Some of these X-ray observations were accompanied by simultaneous optical observations. Relationships between the hardness of the X-ray spectrum and the X-ray intensity and between the hardness and the optical luminosity are compiled. The relationships among the parameters (temperature, density and size) which characterize the postulated isothermal cloud model of SCO X-1 are given. They indicate that SCO X-1 is characterized by a temperature of about 10⁷–10⁸K, a density of about 10¹⁶–10¹⁷ cm^–3 and a radius of about 10⁸–10⁹ cm respectively. We further show that the temperature is inversely correlated with the size of the source; an increase in temperature corresponds to a decrease in the radius and an increase in density.     

Time variations of hard X-rays from Sco X-1

Simultaneous hard X-ray and optical observations of Sco X-1 were carried out on 1971 May 1 at Hyderabad, India, when Sco X-1 was optically bright. The X-ray intensity observed by balloon-borne counter telescopes increased in coincidence with optical enhancements, while the plasma temperature derived by fitting the X-ray spectrum in the energy range 20–40 keV to the thermal bremsstrahlung spectrum did not appreciably change over the whole period of observation.     

Seasonal barotropic sea surface height fluctuation in relation to regional ocean mass variation

A consistency between seasonal fluctuation of actual sea surface height (SSH) and those caused by mass and density variations in gyre-scale regions is examined. The SSH obtained from satellite altimetry (altimetric SSH) is adopted as the actual SSH. SSH caused by mass variation (mass-related SSH) is simulated using a barotropic global ocean model forced by water flux, wind stress and surface pressure. SSH caused by density variation (steric SSH) is calculated from water density profile, i.e. temperature and salinity profiles. The model SSH well represents mass-related SSH for gyre-scale regional means, and seasonal fluctuation of the altimetric SSH corrected for the model SSH is similar to that of steric SSH above a pressure level larger than 300 dbar. The results indicate that the mass-related SSH does not much respond to the baroclinic adjustment to the seasonally varying wind stress curl. The mass-related SSH forced by wind stress and surface pressure should be accounted for regional evaluation, though it is not necessary for global mean evaluation. Detection of steric SSH from altimetric SSH would be useful for assimilation approaches in which the altimetric SSH is treated as the variable reflecting subsurface temperature and salinity.     

Biotic response of benthic foraminifera in Aso-kai lagoon,central Japan,to changes in terrestrial climate and ocean conditions (~AD 700–1600)

We investigated responses of shallow-water benthic foraminifera to changes in climate and ocean conditions, using sediment core ASC2 from Aso-kai lagoon, central Japan. Six AMS ¹⁴C dates reveal that the studied interval corresponds to sediments deposited from ~AD 700 to 1600. Sulfur content of the bulk sediment and multi-dimensional scaling (MDS) axis 1 of fossil benthic foraminifera indicate that the composition of the benthic foraminifera community was closely related to dissolved oxygen (DO) concentration in the hypolimnion. The sulfur content and MDS axis 1 also revealed two shifts over the 900-year interval. In the first phase (~AD 700–1250), the Shannon–Wiener Index (H′), E (S₂₀₀), evenness and rank abundance curve (RAC) kurtosis indicate a gradual deterioration in structure of the benthic foraminifera community. In that period, there are statistically significant correlations between the faunal composition (MDS axis 1) and faunal structure [Shannon–Wiener (H′), E (S₂₀₀), evenness and RAC kurtosis]. In the second phase (~AD 1250–1600), however, faunal composition and structure show no marked correspondence. Instead, abundance of benthic foraminifera fluctuated on a scale of ~200 years. Thus, a shift in the biotic response of benthic foraminifera in Aso-kai lagoon occurred in ca. AD 1250. Gradual deterioration of benthic foraminifera, with taxonomic losses, is consistent with declining DO in the first phase, possibly associated with the increasing influence of the Tsushima Warm Current. The possibility that closure of Aso-kai lagoon and development of the sand bar affected benthic foraminifera cannot, however, be ruled out. No corresponding response was observed in the second phase, during which there was no distinct taxonomic loss. Large variations in abundance, however, were a consequence of strength of the East Asian summer and winter monsoons. The shift in the biotic response of benthic foraminifera in Aso-kai lagoon during the period AD 700–1600 was apparently a result of changes in climate and ocean conditions on the East Asian continental margin.     

Mineralogy and petrography of C asteroid regolith: The Sutter's Mill CM meteorite

Based upon our characterization of three separate stones by electron and X‐ray beam analyses, computed X‐ray microtomography, Raman microspectrometry, and visible‐IR spectrometry, Sutter's Mill is a unique regolith breccia consisting mainly of various CM lithologies. Most samples resemble existing available CM2 chondrites, consisting of chondrules and calcium‐aluminum‐rich inclusion (CAI) set within phyllosilicate‐dominated matrix (mainly serpentine), pyrrhotite, pentlandite, tochilinite, and variable amounts of Ca‐Mg‐Fe carbonates. Some lithologies have witnessed sufficient thermal metamorphism to transform phyllosilicates into fine‐grained olivine, tochilinite into troilite, and destroy carbonates. One finely comminuted lithology contains xenolithic materials (enstatite, Fe‐Cr phosphides) suggesting impact of a reduced asteroid (E or M class) onto the main Sutter's Mill parent asteroid, which was probably a C class asteroid. One can use Sutter's Mill to help predict what will be found on the surfaces of C class asteroids such as Ceres and the target asteroids of the OSIRIS‐REx and Hayabusa 2 sample return missions (which will visit predominantly primitive asteroids). C class asteroid regolith may well contain a mixture of hydrated and thermally dehydrated indigenous materials as well as a significant admixture of exogenous material would be essential to the successful interpretation of mineralogical and bulk compositional data.     

Observation of cosmic soft X-rays

Cosmic soft X-rays in the energy range between 0.14 and 7 keV were observed with thin polypropylene window proportional counters on board a sounding rocket. The field of view crossed the galactic plane in the Cygnus-Cassiopeia region at a large angle and reached the galactic latitudes of –55° and +30°. Referring also to the result with Be window counters, we obtained the energy spectrum of Cyg XR-2, the flux from the Cas A region and the distribution of the intensity of diffuse X-rays over the scanned region. The turn-over of the Cyg XR-2 spectrum at about 1 keV indicates that the distance of the Cyg XR-2 source lies between 600 and 800 pc, if the turn-over is due entirely to interstellar absorption. The flux from the Cas A region is obtained as 0.23±0.05 photons cm^–2 sec^–1 in the energy range between 1.1 and 4.1 keV. The intensity of diffuse soft X-rays depends on the galactic latitude more weakly than expected from the interstellar absorption of extragalactic X-rays and shows asymmetry with respect to the galactic equator, thus suggesting a contribution of galactic X-rays. The spectrum of extragalactic X-rays is approximately represented by a power lawE ^–1.8.