Polybrominated diphenyl ethers do not affect metamorphosis but alter the proteome of the invasive slipper limpet Crepidula onyx

Man-made polybrominated diphenyl ethers (PBDEs) used as flame retardants in various consumer products may be harmful to marine organisms. Larvae of some marine invertebrates, especially invasive species, can develop resistance to PBDEs through altered protein expression patterns or proteome plasticity. This is the first report of a proteomics approach to study BDE-47 induced molecular changes in the invasive limpet Crepidula onyx. Larvae of C. onyx were cultured for 5 days (hatching to metamorphosis) in the presence of BDE-47 (1 μg L⁻¹). Using a 2-DE proteomics approach with triple quadrupole and high-resolution TOF-MS, we showed that BDE-47 altered the proteome structure but not the growth or metamorphosis of C. onyx larvae. We found eight significant differentially expressed proteins in response to BDE-47, deemed the protein expression signature, consisting of cytoskeletal, stress tolerance, metabolism and energy production related proteins. Our data suggest C. onyx larvae have adequate proteome plasticity to tolerate BDE-47 toxicity.     

Effect of transect location,transect spacing and interpolation methods on river bathymetry accuracy

Digital elevation models (DEMs) of river channel bathymetries are developed by interpolating elevations between data collected at discrete points or along transects. The accuracy of interpolated bathymetries depends on measurement error, the density and distribution of point data, and the interpolation method. Whereas point measurement errors can be minimized by selecting the most efficient equipment, the effect of data density and interpolation method on river bathymetry is relatively unknown. Thus, this study focuses on transect‐based collection methods and investigates the effects of transect location, the spacing between transects, and interpolation methods on the accuracy of interpolated bathymetry. This is accomplished by comparing four control bathymetries generated from accurate and high resolution, sub‐meter scale data to bathymetries interpolated from transect data extracted from the control bathymetries using two transect locating methods and four interpolation methods. The transect locating methods are a morphologically‐spaced and an equally‐spaced model. The four interpolation methods are Ordinary Kriging, Delaunay Triangulation, and Simple Linear, which are applied in curvilinear coordinates (Delaunay Triangulation is also applied in Cartesian coordinates), and Natural Neighbor only in Cartesian Coordinates. The bathymetric data were obtained from morphologically simple and complex reaches of a large (average bankfull width = 90 m) and a small (average bankfull width = 17 m) river. The accuracy of the developed DEMs is assessed using statistical analysis of the differences between the control and interpolated bathymetries and hydraulic parameters assessed from bankfull water surface elevations. Results indicate that DEM accuracy is not influenced by the choice of transect location method (with same averaged cross‐section spacing) or a specific interpolation method, but rather by the coordinate system for which the interpolation method is applied and the spacing between transects. They also show negligible differences between the mean depths and surface areas calculated from bathymetries with dense or coarse spacing. Copyright © 2016 John Wiley & Sons, Ltd.     

Infrared dark clouds as precursors to star clusters

Infrared dark clouds (IRDCs) are cold, dense molecular clouds identified as extinction features against the bright mid-infrared Galactic background. Our recent 1.2 mm continuum emission survey of IRDCs reveals many compact (<0.5 pc) and massive (10–2100 M_⊙) cores within them. These prestellar cores hold the key to understanding IRDCs and their role in star formation. Here, we present high angular resolution spectral-line and mm/sub-mm continuum images obtained with the IRAM Plateau de Bure Interferometer and the Sub-Millimeter Array towards three high-mass IRDC cores. The high angular resolution images reveal that two of the cores are resolved into multiple, compact protostellar condensations, while the remaining core contains a single, compact protostellar condensation with a very rich molecular spectrum, indicating that it is a hot molecular core. The derived gas masses for these condensations suggest that each core is forming at least one high-mass protostar, while two of the cores are also forming lower-mass protostars. The close proximity of multiple protostars of disparate mass indicates that these IRDCs are in the earliest evolutionary states in the formation of stellar clusters.     

Fragmentation of rangelands: Implications for humans, animals, and landscapes

Fragmentation of the ecosystems of the earth into spatially isolated units has emerged as a primary component of global change. Often, fragmentation results from actions that are intended to enhance human livelihoods and well-being; however, there are often costs to ecosystems and human economies that are not considered. We describe the three general categories of processes causing fragmentation of rangelands worldwide: dissection, decoupling, and compression. We show that access to heterogeneity of landscapes is an important attribute of grazing ecosystems worldwide, and that fragmentation of these systems, even when it proceeds in the absence of habitat loss, can limit options of people and animals, options that are particularly important in temporally heterogeneous environments. We discuss the consequences of fragmentation for people, livestock, wildlife, and landscapes and describe potential adaptations that can mitigate its harmful outcomes. We close by reviewing policy options that promote re-aggregation of landscapes and adaptation to fragmentation.     

The significance of groundmass ilmenite and megacryst ilmenite in kimberlites

Criteria are suggested for distinguishing xenocrystic ilmenites from those indigenous to the host kimberlite. For instance, in contrast to groundmass grains, ilmenite xenocrysts usually are larger, have reaction rims of leucoxene and perovskite, exhibit strong magnesium enrichment outward, and sometimes have exsolution lamellae and deformation features. Most of the abundant ilmenite macrocrysts found in kimberlite appear to have been phenocrysts in a crystal mush unrelated to kimberlite. On the other hand, kimberlitic groundmass ilmenite is rare, but consistently more magnesian than the cores of macrocrysts. Strong Mg-enrichment patterns evident in the ilmenite macrocrysts probably developed during their attempt to equilibrate with the more magnesian, fractionating kimberlitic liquid. The hypothesis of extensive reaction of ilmenite with kimberlite melt/ fluid has implications with regard to the following: (1) the degree of differentiation of kimberlite melts; (2) the genesis of mantle megacrysts; (3) the reactivity of kimberlite; and (4) the usefulness of groundmass ilmenite as a petrogenetic indicator.     

Anomalies in temperature and rainfall during warm Arctic seasons as a guide to the formulation of climate scenarios

Recently much concern has been expressed regarding the impact of an increased atmospheric CO₂ concentration on climate. Unfortunately, present understanding and models of the climate system are not good enough for reliable prediction of such impacts. This paper presents an analysis of recent climate data in order to illustrate the nature of regional temperature and rainfall changes in different seasons and to provide some guidance with regard to points which might be borne in mind when scenarios of future climate (especially those taking into account human impacts) are being formulated.Since it is believed that an increased atmospheric CO₂ concentration will cause a warming and models and data suggest that the Arctic is more sensitive to climatic change than other latitudes, anomalies associated with warm Arctic seasons have been studied.The regional temperature, precipitation and pressure anomalies in the northern hemisphere for the 10 warmest Arctic winters and 10 warmest Arctic summers during the last 70 years have been investigated. Even when the Arctic area is warm, there are circulation changes such that large coherent anomalies occur elsewhere, with some regions warming and some cooling. The 10 warmest Arctic winters were characterised by larger amplitude anomalies, in the Arctic and elsewhere, than the 10 warmest summers, illustrating the difference in response between seasons. The precipitation differences for the 10 warmest Arctic winters and summers show for North America large coherent areas of increase or decrease, which again differ according to season. However, in winter the differences are not statistically significant, while the differences in two areas are significant in summer.     

Crater size-shape profiles for the moon and mercury: Terrain effects and interplanetary comparisons

New crater size-shape data were compiled for 221 fresh lunar craters and 152 youthful mercurian craters. Terraces and central peaks develop initially in fresh craters on the Moon in the 0–10 km diameter interval. Above a diameter of 65 km all craters are terraced and have central peaks. Swirl floor texture is most common in craters in the size range 20–30 km, but it occurs less frequently as terraces become a dominant feature of crater interiors. For the Moon there is a correlation between crater shape and geomorphic terrain type. For example, craters on the maria are more complex in terms of central peak and terrace detail at any given crater diameter than are craters in the highlands. These crater data suggest that there are significant differences in substrate and/or target properties between maria and highlands. Size-shape profiles for Mercury show that central peak and terrace onset is in the 10–20 km diameter interval; all craters are terraced at 65 km, and all have central peaks at 45 km. The crater data for Mercury show no clear cut terrain correlation. Comparison of lunar and mercurian data indicates that both central peaks and terraces are more abundant in craters in the diameter range 5–75 km on Mercury. Differences in crater shape between Mercury and the Moon may be due to differences in planetary gravitational acceleration (gMercury=2.3gMoon). Also differences between Mercury and the Moon in target and substrate and in modal impact velocity may contribute to affect crater shape.     

A casting procedure for reproducing coarse‐grained sedimentary surfaces

In the ?eld, the measurement of near‐bed hydraulics remains problematic. Greater precision is possible in the laboratory, but, in the case of gravels, it is dif?cult to create a water‐worked channel‐bed that is realistic enough to replicate faithfully the conditions found in nature. In this paper, a technique to reproduce coarse‐grained sedimentary fabrics of large areal extent is described. It involves moulding natural river‐bed surfaces from which facsimiles are cast. Remarkably realistic casts with dimensions of 1 m by 2 m have been produced and their quality assessed using spatial data derived using automated digital photogrammetry. The casts reproduce the prototype surfaces with errors at millimetre scale (0·5 per cent of the microrelief). The technique has facilitated the introduction of sedimentary surfaces that incorporate natural, complex structures of grains up to cobble size into experimental channels where detailed studies of near‐bed hydraulics can be carried out. Copyright © 2003 John Wiley & Sons, Ltd.