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Test and evaluation of a moored microstructure recorder

A new moored microstructure recorder （MMR） is designed, developed, tested, and evaluated. The MMR directly measures the high-frequency shear of velocity fluctuations, with which we can estimate the dissipation rate of turbulent kinetic energy. We summarize and discuss methods for estimating the turbulent kinetic energy dissipation rate. Instrument body vibrations contaminate the shear signal in an ocean field experiment, and a compensating correction successfully removes this contamination. In both tank test and ocean field experiment, the dissipation rate measured with the MMR agreed well with that measured using other instruments.     

Measurement of trace nitrate concentrations in seawater by ion chromatography with valve switching

An ion chromatographic method with a valve switching facility was developed to determine trace nitrate concentrations in seawater using two pumps, two different suppressors, and two columns. A carbohydrate membrane desalter was used to reduce the high concentrations of sodium salts in samples. In this method, trace nitrate was eluted from the concentrator column to the analytical columns, while the matrix flowed to waste. Neither chemical pre-treatment nor sample dilution was required. In the optimized separation conditions, the method showed good linearity （R〉0,99） in the 0.05 and 50 mg/L concentration range, and satisfactory repeatability （RSD〈5%, n=6）. The limit of detection for nitrate was 0.02 mg/L. Results showed that the valve switching system was suitable and practical for the determination of trace nitrate in seawater.     

Impacts of internal waves on chlorophyll a distribution in the northern portion of the South China Sea

Internal waves can bring nutrients to the upper level of water bodies and facilitate phytoplankton photosynthesis. Internal waves occur frequently in the northern portion of the South China Sea and inflict an important effect on chlorophyll a distribution. In this study, in-situ observation and satellite remote sensing data were used to study the effects of internal waves on chlorophyll a distribution. Based on the in-situ observations, lower chlorophyll a concentrations were present in the middle and bottom level in areas in which internal waves occur frequently, while the surface chlorophyll a distribution increased irregularly, and a small area with relatively higher chlorophyll a concentrations was observed in the area around the Dongsha Island. Satellite remote sensing showed that the chlorophyll a concentration increased in the area near Dongsha Island, where internal waves frequently occurred. The results of the increased chlorophyll a concentration in the surface water near Dongsha Island in the northern portion of the South China Sea indicated that internal waves could uplift phytoplankton and facilitate phytoplankton growth.     

Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

In this study, we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay. Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h. The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%, respectively, and the half-inhibitory concentration of PE (IC₅₀) on P388 and BEL-7402 cells was 120 μg/mL and >200 μg/mL, respectively. Microscopic observation shows that the morphologic features of tumor cells treated with PE and 5-fluorouracil are markedly different from the normal control group. The inhibitory rate of fraction A₂ isolated from PE by sephadex LH-20 for BEL-7402 and P388 cells at the sample concentration of 70.42 μg/mL was 61.96±7.02% and 40.47±8.70%, respectively. The apoptosis peak for fraction A₂ was the most profound of all fractions used in the flow cytometry assay. The results indicate that the anti-proliferative of this algal extract is associated with the total phlorotannin content.     

Effects of rearing temperature and density on growth, survival and development of sea cucumber larvae, Apostichopus japonicus (Selenka)

In laboratory conditions, effects of rearing temperature and stocking density were examined on hatching of fertilized egg and growth of auricularia larvae of Apostichopus japonicus respectively. Data series like larval length and density, metamorphic time, and survival rate of the larvae were recorded. Statistics showed that for A. japonicus, survival rate (from fertilized egg to late auricularia) decreased significantly with the increasing rearing temperature (P<0.05). At different temperatures SGR was statistically significant as well (P<0.05) from day 1, and maximal SGR was found on day 9 at 24°C (159.26±3.28). This study clearly indicated that at low temperature (<24°C), metamorphic rate was remarkably higher than at higher temperature (>26°C). Hatching rate was significantly different between 0.2–5 ind./ml groups and 20–50 ind./ml groups. Rearing larvae at the higher density had the smaller maximal-length, whereas needed longer time to complete metamorphosis. This study suggested that 21°C and 0.4 ind./ml can be used as the most suitable rearing temperature and stocking density for large -scale artificial breeding of A. japonicus’s larvae.     

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TEQC?????DOS?????????????????TEQC????????????????????????????????????????????????????м??????????????????????????????????????????????????????Visual Basic.NET??TEQC????Windows?????????????????????????????????????????????????????????????????????????     

联合GPS和GRACE数据分析陕甘宁地区地表垂直形变

采用甘肃省CORS网和中国大陆构造环境监测网络中共48个台站的GPS观测数据，解算得到观测台站的垂直位移，并与GRACE时变重力场Mascon模型解CSR RL05M数据计算得到的垂直形变进行比较，分析区域地表垂直形变特征。结果表明，研究区内台站垂直形变存在局部特征，甘肃庆阳和平凉地区垂直形变与其他地区存在明显差异，相关系数、均方根减少量和周年信号减少量均高于其他地区；扣除趋势项后，观测台站GPS垂直位移与GRACE垂直形变时间序列相关系数均值为0.72，GPS和GRACE周年信号振幅均值分别为6.00 mm和3.70 mm，周年信号减少量和均方根误差减少量均值分别为0.51和0.29；研究区内GPS垂直位移和GRACE垂直形变时间序列一致性较强，GRACE垂直形变能有效解释50%以上的GPS垂直位移周年信号，GPS垂直位移时间序列包含的非构造形变中平均约29%来源于环境负载变化所引起的负荷形变。     

涠西南凹陷流沙港组烃源岩生产力及发育模式

涠西南凹陷是中国近海北部湾盆地已证实的富烃凹陷,始新统流沙港组是该凹陷主要烃源岩层系.为深入认识涠西南凹陷流沙港组烃源岩的生产力和发育特征,采用有机地球化学与地球生物学相结合、定性分析与定量计算相结合的方法正演恢复了涠西南凹陷不同次洼流沙港组不同层段烃源岩的古生产力、有机质埋藏效率和有机碳埋藏生产力,进而建立了研究区流沙港组烃源岩形成的地球生物学模式.结果表明,涠西南凹陷流沙港组烃源岩的古生产力、有机质埋藏效率及有机碳埋藏生产力在横向不同次洼和纵向不同层段上均存在差异,横向上以B次洼最优,纵向上以流二段最高;涠西南凹陷流沙港组烃源岩发育超营养湖高埋藏效率高埋藏生产力、富营养湖中等埋藏效率中等埋藏生产力及富营养湖低埋藏效率低埋藏生产力3种代表性的地球生物学模式.