Application of enterococci antibiotic resistance patterns for contamination source identification at Huntington Beach,California

Huntington Beach, California, one of the most popular surfing spots in the world, is plagued by sporadic, elevated levels of fecal bacteria. To assist with pollution source identification, we analyzed antibiotic resistance patterns (ARPs) of enterococci from four known sources (bird feces, urban runoff, coastal marsh sediment and sewage effluent from local sanitation district) and one unknown source (seawater) using seven antibiotics at four concentrations each. Of 2491 enterococci tested, all were resistant to at least one antibiotic at some level. Discriminant analysis indicated that the average correct classification rates for bird feces and urban runoff sources were above 80%. Sewage effluent contained mixed fecal sources. Sixty-four percent of the sewage isolates classified with the sewage category, while the other 35% of isolates were assigned evenly across the other three categories. When enterococci isolated from the seawater were classified using the known ARP database, it was evident that bird feces were the source of surf zone contamination on some days while the coastal salt marsh and sewage plume may have impacted the surf zone water quality to various degrees during other times.     

Environmental management of marine fish culture in Hong Kong

Marine fish farming is an important commercial practice in Hong Kong. Marine fish farms located in eutrophic coastal waters often face the threat of severe dissolved oxygen depletion associated with algal blooms and red tides. On the other hand, mariculture activities also contribute to pollution. The sustainable management of mariculture requires proper siting of the fish farms and stocking density control. Both of these are related to the carrying capacity of the water body concerned, which is mainly governed by its flushing characteristics. A simple method to determine the carrying capacity of a fish farm has been developed by using three-dimensional (3D) hydrodynamic modelling and its effective coupling with a diagenetic water quality model. A systematic methodology using numerical tracer experiments has been developed to compute the tidal flushing in a fish farm. The flushing time is determined from the results of a numerical tracer experiment using robust 3D hydrodynamic and mass transport models. A unit tracer concentration is initially prescribed inside the region of interest and zero elsewhere; the subsequent mass transport and the mass removal process are then tracked. The fish farms are usually situated in well-sheltered shallow embayments and may not connect directly to the open water. It is found that it is necessary to define both "local" and "system-wide" flushing times to represent the effectiveness of the mass exchange with the surrounding water body and the open sea respectively. A diagenetic water quality model simulating the sediment-water-pollutant interaction is employed to address the response of the water column and the benthic layer to pollution discharges. With the flushing rate reliably computed, the carrying capacity of the fish farm can be determined in terms of key water quality parameters: chlorophyll-a, dissolved oxygen, organic nitrogen and potential lowest dissolved oxygen level on a day of negligible photosynthetic production. The predictions are well-supported by field data.     

Estimates of Tsunami Risk Zones on the Coasts Adjacent to the East (Japan) Sea based on the Synthetic Catalogue

Prognostic characteristics of tsunamis in the East (Japan) Sea based on numerical simulations are investigated by using linear long wave theory. Due to the lack of observed data, the concept of the synthetic catalogue is applied to generate possible tsunami scenarios. It includes four real events that occurred in the East (Japan) Sea during the 20th century, 24 hypothetical tsunamigenic earthquakes located in the gap zones of the seismic map, and 76 idealized model ‘hydrodynamic’ sources covering the eastern part of the East (Japan) Sea uniformly. The tsunami wave height distributions along the East (Japan) Sea coastline due to these hypothetical events are computed. From the geographical distributions of tsunami wave height for all possible events, it is found that there exist several coastal locations where the tsunami risk is relatively lower than in other zones. The relation between the maximal value of the tsunami height and its average value is analyzed. It is found that the maximal tsunami height does not exceed the mean wave height times a constant. The uniform bounded curve for all areas can be obtained if the mean wave height is replaced by the modified mean wave height (1/3 of largest waves). The problem of quantitative definition of the prognostic tsunami wave height for each location based on the data from the synthetic catalogue is discussed. The results of tsunami wave height analysis based on the synthetic catalogue can be used as a tool for coastal disaster mitigation planning.     

240-kHz bistatic bottom scattering measurements in shallow water

High-frequency bistatic sediment scattering experiment was conducted in the shallow waters off the east coasts of Korea. Acoustic data were taken as a function of grazing angle (30°, 45°, and 60°), scattered angle (30°, 45°, and 60°), and bistatic (azimuthal) angle (0°, 60°, and 120°). Besides a flat bottom it was artificially raked so as to produce directional ripples. The measured scattering strengths for a flat bottom were compared to model predictions of D.R. Jackson et al. (1986). The surface reverberation component is seen to dominate over the volume scattering part at the frequency of 240 kHz. Compared to the flat bottom case, the scattering strengths for directional ripples showed lower and higher variation depending on the ripple's orientation     

Screening of surfactants for harmful algal blooms mitigation

Screening experiments were conducted in order to find promising synthetic surfactants for harmful algal blooms (HABs) mitigation. The chemically synthesized surfactant cocamidopropyl betaine (CAPB) showed characteristics of relatively high inhibition efficiency, high biodegradability and low cost. The motility inhibition ratios of 10 mg/L CAPB on Cochlodinium polykrikoides and Alexandrium tamarense were about 60% after 5 min. The biodegradation test indicated that the half-life of CAPB in seawater was shorter than one day and 90% was biodegraded after five days under the initial concentration of 100 mg/L at 25 degrees C. Further cell lysis experiments revealed the selective lysis effect of CAPB on different HAB organisms. More than 90% of C. polykrikoides lysed at the concentration of 10 mg/L CAPB after 24 h and at 15 mg/L CAPB after 4 h, whereas the lysis effect of CAPB on A. tamarense was slight, no more than 10% after 2 h interaction with 50 mg/L CAPB. This research provided preliminary data for CAPB as a candidate in harmful algal blooms mitigation and pointed out unresolved problems for its practical application in the meantime.     

Okadaic acid, a causative toxin of diarrhetic shellfish poisoning, in green-lipped mussels Perna viridis from Hong Kong fish culture zones: Method development and monitoring

Green-lipped mussels (Perna viridis) were collected from seven fish culture zones (FCZs) in Hong Kong and analyzed for okadaic acid (OA). A conventional HPLC method was modified by incorporating a proteinase K digestion step. Results suggest that a higher recovery (2.5 times higher) of OA was obtained from spiked samples after the addition of 1.08 mg proteinase K in comparison with samples incubated without the proteolytic enzyme. For the hepatopancreas (HP) of individual field-collected mussels, the additional digestion step can enhance OA extraction by 3.1 times. Spatial and temporal variations in OA concentrations in the mussels from various FCZs were investigated. The highest concentration of OA in mussel HP samples was 1164.9 ng/g HP wet wt. With respect to OA concentrations in whole mussel tissues from seven sites and four seasonal samplings, the concentrations were between 70.0 and 131.0 ng/g wet wt., which did not exceed the generally recognized international regulatory criteria (>200 ng/g) for OA.     

Assessing the role of North Atlantic freshwater forcing in millennial scale climate variability: a tropical Atlantic perspective

This study analyzes a three-member ensemble of experiments, in which 0.1 Sv of freshwater was applied to the North Atlantic for 100 years in order to address the potential for large freshwater inputs in the North Atlantic to drive abrupt climate change. The model used is the GFDL R30 coupled ocean–atmosphere general circulation model. We focus in particular on the effects of this forcing on the tropical Atlantic region, which has been studied extensively by paleoclimatologists. In response to the freshwater forcing, North Atlantic meridional overturning circulation is reduced to roughly 40% by the end of the 100 year freshwater pulse. Consequently, the North Atlantic region cools by up to 8°C. The extreme cooling of the North Atlantic increases the pole-to-equator temperature gradient and requires more heat be provided to the high latitude Atlantic from the tropical Atlantic. To accommodate the increased heat requirement, the ITCZ shifts southward to allow for greater heat transport across the equator. Accompanying this southward ITCZ shift, the Northeast trade winds strengthen and precipitation patterns throughout the tropical Atlantic are altered. Specifically, precipitation in Northeast Brazil increases, and precipitation in Africa decreases slightly. In addition, we find that surface air temperatures warm over the tropical Atlantic and over Africa, but cool over northern South America. Sea-surface temperatures in the tropical Atlantic warm slightly with larger warm anomalies developing in the thermocline. These responses are robust for each member of the ensemble, and have now been identified by a number of freshwater forcing studies using coupled OAGCMs. The model responses to freshwater forcing are generally smaller in magnitude, but have the same direction, as paleoclimate data from the Younger Dryas suggest. In certain cases, however, the model responses and the paleoclimate data directly contradict one another. Discrepancies between the model simulations and the paleoclimate data could be due to a number of factors, including inaccuracies in the freshwater forcing, inappropriate boundary conditions, and uncertainties in the interpretation of the paleoclimate data. Despite these discrepancies, it is clear from our results that abrupt climate changes in the high latitude North Atlantic have the potential to significantly impact tropical climate. This warrants further model experimentation into the role of freshwater forcing in driving climate change.     

Distribution Functions of Tsunami Wave Heights

The problem of describing the distribution functions of tsunami wave heights is discussed. Data on runup heights obtained in field surveys of several tsunamis for the last decade are used to calculate the empirical distribution functions. It is shown that the log-normal distribution describes the observed data well. This means that the irregular topography and coastline are major factors which influence the height distribution. The power distribution related with the geometric decay of the propagated wave is a good approximation for one event (Sulawesi, January 1, 1996) only. Results of a numerical simulation of the tsunami event in the Japan (East) Sea on July 12, 1993 are presented. It is shown that the computed wave height distribution, obtained by using the runup correction in the framework of nonlinear shallow-water theory, is in good agreement with the observed height distribution. Simulations are used to study the transformation of the distribution function on different distances from the source.     

Fe and Mn levels regulated by agricultural activities in alluvial groundwaters underneath a flooded paddy field

Iron and Mn concentrations in fresh groundwaters of alluvial aquifers are generally high in reducing conditions reflecting low SO₄ concentrations. The mass balance and isotopic approaches of this study demonstrate that reduction of SO₄, supplied from agricultural activities such as fertilization and irrigation, is important in lowering Fe and Mn levels in alluvial groundwaters underneath a paddy field. This study was performed to investigate the processes regulating Fe and Mn levels in groundwaters of a point bar area, which has been intensively used for flood cultivation. Four multilevel-groundwater samplers were installed to examine the relationship between geology and the vertical changes in water chemistry. The results show that Fe and Mn levels are regulated by the presence of NO₃ at shallow depths and by SO₄ reduction at the greater depths. Isotopic and mass balance analyses revealed that NO₃ and SO₄ in groundwater are mostly supplied from the paddy field, suggesting that the Fe-and Mn-rich zone of the study area is confined by the agricultural activities. For this reason, the geologic conditions controlling the infiltration of agrochemicals are also important for the occurrence of Fe/Mn-rich groundwaters in the paddy field area.