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21.
J.A. Saunders M.-K. Lee M. Shamsudduha P. Dhakal A. Uddin M.T. Chowdury K.M. Ahmed 《Applied Geochemistry》2008
Here new data from field bioremediation experiments and geochemical modeling are reported to illustrate the principal geochemical behavior of As in anaerobic groundwaters. In the field bioremediation experiments, groundwater in Holocene alluvial aquifers in Bangladesh was amended with labile water-soluble organic C (molasses) and MgSO4 to stimulate metabolism of indigenous SO4-reducing bacteria (SRB). In the USA, the groundwater was contaminated by Zn, Cd and SO4, and contained <10 μg/L As under oxidized conditions, and a mixture of sucrose and methanol were injected to stimulate SRB metabolism. In Bangladesh, groundwater was under moderately reducing conditions and contained ∼10 mg/L Fe and ∼100 μg/L As. In the USA experiment, groundwater rapidly became anaerobic, and dissolved Fe and As increased dramatically (As > 1000 μg/L) under geochemical conditions consistent with bacterial Fe-reducing conditions. With time, groundwater became more reducing and biogenic SO4 reduction began, and Cd and Zn were virtually completely removed due to precipitation of sphalerite (ZnS) and other metal sulfide mineral(s). Following precipitation of chalcophile elements Zn and Cd, the concentrations of Fe and As both began to decrease in groundwater, presumably due to formation of As-bearing FeS/FeS2. By the end of the six-month experiment, dissolved As had returned to below background levels. In the initial Bangladesh experiment, As decreased to virtually zero once biogenic SO4 reduction commenced but increased to pre-experiment level once SO4 reduction ended. In the ongoing experiment, both SO4 and Fe(II) were amended to groundwater to evaluate if FeS/FeS2 formation causes longer-lived As removal. Because As-bearing pyrite is the common product of SRB metabolism in Holocene alluvial aquifers in both the USA and Southeast Asia, it was endeavored to derive thermodynamic data for arsenian pyrite to better predict geochemical processes in naturally reducing groundwaters. Including the new data for arsenian pyrite into Geochemist’s Workbench, its stability field completely dominates in reducing Eh–pH space and “displaces” other As-sulfides (orpiment, realgar) that have been implied to be important in previous modeling exercises and reported in rare field conditions. 相似文献
22.
Diel patterns in the chlorophyll a specific absorption coefficient of surface picoplankton, a*pico (γ) (m2·[mg chlorophyll a]−1), were studied at 7 stations under daily cycle of in situ light condition in the western subarctic Pacific and Japan Sea. All the data were normalized by dividing the anomaly with daily averaged a*pico (γ). Opposite diel patterns were observed for the normalized a*pico (443) and a*pico (675) with maximum toward dawn or dusk and minimum toward midday at 4 stations under low-irradiance (LI) conditions and vice versa at 3 stations under high-irradiance (HI) conditions. The absorption efficiency factors at red absorption peak, Q
a (675), were determined by reconstruction with intracellular chlorophyll a concentration and cell diameter. The normalized Q
a (675) also showed diel pattern with maximum toward midday and minimum toward dawn or dusk under LI. The diel pattern in a*pico (675) and Q
a (675) were primarily caused by changes in intracellular chlorophyll a concentration due to photoadaptation under LI. The diel pattern in a*pico (443) was influenced by pigmentation, as recognized by blue to red ratio [a*pico (443)/a*pico (675)] under HI. This study proposed that the opposite diel pattern in a*pico (γ) might occur for a wide range of algal species. The results presented here have important consequences for the interpretation of diel variations in optical properties observed in the open ocean. 相似文献
23.
R.F. Lee B. Dornseif F. Gonsoulin K. Tenore R. Hanson 《Marine environmental research》1981,5(2):125-143
Addition of a heavy oil to a Spartina salt marsh in the autumn resulted in high concentrations of polycyclic aromatic hydrocarbons in sediment and benthic animals. The highest concentrations of phenanthrene, chrysene and fluoranthene in the sediment were 112, 105 and 75 ng/g sediment, respectively. These concentrations rapidly decreased during the 20 week period following the spill. The times for these hydrocarbons to decrease to 50% of their highest values, i.e. half-life, were approximately 100, 70 and 30 days in sediment, mussels and oysters, respectively. Benthic macrofauna species showed three responses to oil addition which included no change, an increase, or a decrease in the population. No changes were noted in populations of fiddler crabs (Uca pugnax), oysters (Crassostrea virginica), and mussels (Modiolus demissus). Mud snails (Nassarius obsoleta) increased in density after the spill due to immigration of adult snails from untreated areas to scavenge on animals killed by the oil. Many of the adult periwinkles (Littorina irrorata) were killed by the oil. In the spring, juvenile periwinkles recolonised to oiled areas as a result of larvae settling. 相似文献
24.
Lee Shugart 《Marine environmental research》1988,24(1-4)
This paper reports a rapid and facile procedure for obtaining of DNA from small, whole aquatic organisms (fathead minnow, Pimephales promelas) as well as from the liver of another species (bluegill sunfish, Lepomis macrochirus) and the subsequent estimation of its double strandedness. DNA was isolated from various tissues by homogenization in a basic pH solution containing detergent followed by differential extraction using organic solvents. Additional purification was accomplished by molecular sieve chromatography. Under defined alkaline unwinding conditions, the amount of double-stranded and single-stranded DNA was determined using the bisbenzimidazole dye Hoechst 33258. The technique was used to detect the occurrence of strand breaks in DNA of fathead minnows chemically exposed to benzo[a]pyrene. 相似文献
25.
In this study, we investigate two internal wave generation methods in numerical modeling of time-dependent equations for water wave propagation, i.e., delta source function method and source term addition method, the latter of which has been called the line source method in literatures. We derive delta source functions for the Boussinesq-type equations and extended mild-slope equations. By applying the fractional step splitting method, we show that the delta source function method is equivalent to the source term addition method employing the energy velocity. This suggests that the energy velocity should be used rather than the phase velocity for the transport of incident wave energy in the source term addition method. Finally, the performance of the delta source function method is verified by accurately generating nonlinear cnoidal waves as well as linear waves for horizontally one-dimensional cases. 相似文献
26.
We develop techniques of numerical wave generation in the time-dependent extended mild-slope equations of Suh et al. [1997. Time-dependent equations for wave propagation on rapidly varying topography. Coastal Engineering 32, 91–117] and Lee et al. [2003. Extended mild-slope equation for random waves. Coastal Engineering 48, 277–287] for random waves using a source function method. Numerical results for both regular and irregular waves in one and two horizontal dimensions show that the wave heights and the frequency spectra are properly reproduced. The waves that pass through the wave generation region do not cause any numerical disturbances, showing usefulness of the source function method in avoiding re-reflection problems at the offshore boundary. 相似文献
27.
Laboratory radiotracer experiments were conducted to determine assimilation efficiencies (AE) from ingested algal food and oxic sediment particles, uptake rates from the dissolved phase, and the efflux rates of Cd, Cr and Zn in the Asiatic clamCorbicula fluminea. Among three elements, AE from both algal and sediment food was greatest for Cd, followed by Zn and Cr. The AEs of tested elements from algal food(Phaeodactylum tricornutum) were consistently higher than those from sediments at a given salinity and temperature. The influence of salinity (0, 4 and 8 psu) and temperature (5, 13 and 21¼) on the metal AEs was not evident for most tested elements, except Cd AEs from sediment. The rate constant of metal uptake from the dissolved phase (k u was greatest for Cd, followed by Zn and Cr in freshwater media. However, in saline water, thek u of Zn were greater than those of Cd. The influx rate of all tested metals increased with temperature. The efflux rate constant was greatest for Cr (0.02 d-1), followed by Zn (0.010~0.017 d-1) and Cd (0.006 d-1). The efflux rate constant for Zn in clam tissues depurated in 0 psu (0.017 d-1) was faster than that in 8 psu (0.010 d-1). Overall results showed that the variation of salinity and temperature in estuarine systems can considerably influence the metal bioaccumulation potential in the estuarine clamC. fluminea. The relatively high Cd accumulation capacity ofC. fluminea characterized by the high AE, high dissolved influx rate and low efflux rate, suggested that this clam species can be used as an efficient biomonitor for the Cd contamination in freshwater and estuarine environments. 相似文献
28.
Toru?Iwata Keiko?Yoshikawa Katsutoshi?Nishimura Yoshihisa?Higuchi Takao?Yamashita Shigeru?Kato Eiji?OhtakiEmail author 《Journal of Oceanography》2004,60(6):995-1000
The measurements of the vertical transport of CO2 were carried out over the Sea of Japan using the specially designed pier of Kyoto University on September 20 to 22, 2000. CO2 fluxes were measured by the eddy correlation and aerodynamic techniques. Both techniques showed comparable CO2 fluxes during sea breeze conditions: −0.001 to −0.08 mg m−2s−1 with the mean of −0.05 mg m−2s−1. This means that the measuring site satisfies the fetch requirement for meteorological observations under sea breeze conditions. Moreover, the eddy diffusivity coefficient used in the aerodynamic technique is found to be consistent with the coefficient used in the eddy correlation technique. The present result leads us to conclude that the aerodynamic technique may be applicable to underway CO2 flux measurements over the ocean and may be used in place of the bulk technique. The important point is the need to maintain a measuring accuracy of CO2 concentration difference of the order of 0.1 ppmv on the research vessels or the buoys. 相似文献
29.
Monthon?Ganmanee Teruyoshi?Narita Hideo?SekiguchiEmail author 《Journal of Oceanography》2004,60(6):1071-1083
Based on our long-term data from megabenthos sampling from 1993 to 2002 in Ise Bay, central Japan, we examined spatio-temporal variations in taxon composition, species richness and its distribution of megabenthos in the bay in relation to the occurrence of the oxygen-poor water (i.e. oxygen content less than 3 ppm) in bottom waters of the bay. A total of 261 species were identified including 6 cnidarians, 1 tentaculate, 5 annelids, 71 molluscs, 72 crustaceans, 16 echinoderms, 12 urochordates and 78 pisces. Of the most abundant 10 megabenthos species, the following 4 species of echinoderms made up more than the half of megabenthos biomass: Luidia quinaria, Echinocardium cordatum, Asterias amurensis and Astropecten scoparius. Species richness of megabenthos varied significantly between seasons and among stations. The severity and period of occurrence of the oxygen-poor water developing every summer play an important role in determining spatial distributions of species richness in the bay. 相似文献
30.
To use two small fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) and the Japanese medaka Oryzias latipes (Belloniformes) as testing models in molecular ecotoxicology, we have cloned the cytochrome P450 1A (CYP1A) gene after screening of both genomic DNA libraries, and sequenced 11,863 and 7,243 bp including all the exons and introns with promoter regions, respectively. The Rivulus and the medaka CYP1A gene consisted of seven exons (including non-coding exons) with high homology to mammals. In the promoter region, Rivulus CYP1A gene has seven xenobiotic response elements (XREs) and two metal response elements (MREs), while the Japanese medaka CYP1A gene has six XREs and four MREs. Interestingly, medaka CYP1A gene has a number of MREs at the promoter, which may affect its response on metal exposure. We describe here the gene structure of both fish CYP1A genes. 相似文献