Effects of fixation and storage on flow cytometric analysis of marine bacteria

Flow cytometry (FCM) is now becoming a routine tool for the enumeration and optical characterization of bacteria in marine environments. We investigated the effects of sample fixation and storage upon flow cytometric determination of marine bacteria. Fixed and unfixed seawater samples were analyzed by FCM immediately aboard ship and/or later in the laboratory, and the appearances of the fluorescence signals and bacterial counts of these samples were compared. Fixation and storage led to the formation of multiple peaks in fluorescence histograms; this was also seen in 22 out of 36 samples frozen in liquid nitrogen. Fixation did not, but storage did induce a decrease of bacterial counts: a rapid decrease during the first 3 days followed by a slower decline. The decline of cell numbers in stored samples was expressed by a regression model. Our studies indicate that precaution is necessary when interpreting the data from fixed and/or stored marine bacterial samples analyzed by FCM. The possibility that the procedure of fixation and storage leads to the appearance of high DNA and low DNA bacterial groups should be considered.     

Hit or miss: Glacial incisions of snowball Earth

Estimated at ~58 Ma in duration, the Sturtian snowball Earth (ca. 717–659 Ma) is one of the longest‐known glaciations in Earth history. Surprisingly few uncontroversial lines of evidence for glacial incisions associated with such a protracted event exist. We report here multiple lines of geological field evidence for deep but variable glacial erosion during the Sturtian glaciation. One incision, on the scale of several kilometres, represents the deepest incision documented for snowball Earth; another much more modest glacial valley, however, suggests an erosion rate similar to sluggish Quaternary glaciers. The heterogeneity in snowball glacial incisions reported here and elsewhere was likely influenced by actively extending horst‐and‐graben topography associated with the breakup of supercontinent Rodinia.     

Three-dimensional simulations and wind-tunnel experiments on airflow over isolated forest stands

Prediction of windthrow risk to individual or groups of retained trees in harvested stands requires an improved understanding of canopy airflow dynamics. Large-eddy simulations were used to simulate wind-tunnel experiments in two and three dimensions to compare with observations for model validation and to address parameter space considerations for the design of subsequent retention pattern experiments. The three-dimensional simulations were similar to the observed wind-tunnel data for the statistical profiles for but there were greater differences in skewness and kurtosis. These results were obtained using a common leaf-area drag formulation without either skin friction or speed dependent drag that enables scaling with U ₀ (ambient wind speed) and h (height of the canopy). This scaling results in a single non-dimensional parameter h/h _c where h _c (x, y, z) is the momentum range resulting from the canopy drag. The validity of the model scaling was tested using two-dimensional simulations. The irrotational component of the flow (potential flow) was found to be important when defining vertical domain limitations and has significant implications for time dependent flow (i.e. turbulent conditions) when considering retention pattern design. The sudden onset of drag associated with the isolated stand presents some unexpected challenges. The horizontal scales of the shearing instabilities were simulated in two dimensions and found to range between 2h for early times to 7h for later times. The early-time horizontal scales are in the range of logical retention pattern scales and as such need to be taken into account as part of the parameter space, i.e. a range of retention pattern lengths need consideration.     

Palomar and Table Mountain observations of 9P/Tempel 1 during the Deep Impact encounter: First results

We present the first results of the Palomar Adaptive Optics observations taken during the Deep Impact encounter with 9P/Tempel 1 in July 2005. We have combined the Palomar near-IR imaging data with our visual wavelength images obtained simultaneously at JPL's Table Mountain Observatory to cover the total wavelength range from 0.4 to 2.3 μm in the B, V, R, I, J, H, and K filter bands, spanning the dates from 2005 July 03-07. We also include in our overall analysis images taken on the pre-encounter dates of June 1 and June 15, 2005. The broad wavelength range of our observations, along with high temporal resolution, near-IR sensitivity, and spatial resolution of our imaging, have enabled us to place constraints on the temperature of the impact flash and incandescent plume of >700 K, and to provide mean dust velocities of order approximately 1.25 h after impact derived from our 1.64 μm observations. Our ejected dust mass estimates, as derived from our near-IR observations, are an order of magnitude less than those previously reported for visual wavelength observations.     

Neutron diffraction determination of the cell dimensions and thermal expansion of the fluoroperovskite KMgF3 from 293 to 3.6 K

The cell dimensions of the fluoroperovskite KMgF₃ synthesized by solid state methods have been determined by powder neutron diffraction and Rietveld refinement over the temperature range 293–3.6 K using Pt metal as an internal standard for calibration of the neutron wavelength. These data demonstrate conclusively that cubic $ Pm\overline{3} m The cell dimensions of the fluoroperovskite KMgF₃ synthesized by solid state methods have been determined by powder neutron diffraction and Rietveld refinement over the temperature range 293–3.6 K using Pt metal as an internal standard for calibration of the neutron wavelength. These data demonstrate conclusively that cubic KMgF₃ does not undergo any phase transitions to structures of lower symmetry with decreasing temperature. Cell dimensions range from 3.9924(2) ? at 293 K to 3.9800(2) ? at 3.6 K, and are essentially constant within experimental error from 50 to 3.6 K. The thermal expansion data are described using a fourth order polynomial function.     

The rheology of cryovolcanic slurries: Motivation and phenomenology of methanol-water slurries with implications for Titan

The Cassini spacecraft has revealed landforms on the surface of Titan suggested to be viscous cryovolcanic flows and possibly eruptive domes. In order to relate those surface features to the processes and chemistries that produced them, it is necessary to construct flow models, which rely on characterization of the rheological properties of the eruptants. This paper describes our initial exploratory attempts to understand the rheological characteristics of cryogenic slurries, using a 40% methanol-water mixture, as a precursor to more detailed experiments. We have devised a new automated cryogenic rotational viscometer system to more fully characterize cryovolcanic slurry rheologies. A series of measurements were performed, varying first temperature, and then strain rate, which revealed development of yield stress-like behaviors, shear-rate dependence, and thixotropic behavior, even at relatively low crystal fractions, not previously reported. At fixed shear rate our data are fit well by the Andrade equation, with the activation energy modified by a solid volume fraction. At fixed temperature, depending on shearing history, a Cross model could describe our data over a wide shear rate range. A Bingham plastic model appears to be a good constitutive model for the data measured at high shear rates when the shear was global. The yield stress like behavior implies that levee formation on cryolava flows is more likely than would be inferred from the previous studies, and may provide a partial explanation for features interpreted as steep-sided volcanic constructs on Titan.     

The impact of methane thermodynamics on seasonal convection and circulation in a model Titan atmosphere

We identify mechanisms controlling the distribution of methane convection and large-scale circulation in a simplified, axisymmetric model atmosphere of Titan forced by gray radiation and moist (methane) convection. The large-scale overturning circulation, or Hadley cell, is global in latitudinal extent and provides fundamental control of precipitation and tropospheric winds. The precipitating, large-scale updraft regularly oscillates in latitude with seasons. The distance of greatest poleward excursion of the Hadley cell updraft is set by the mass of the convective layer of the atmosphere; convection efficiently communicates seasonal warming of the surface through the cold and dense lower atmosphere, increasing the heat capacity of the system. The presence of deep, precipitating convection introduces three effects relative to the case with no methane latent heating: (1) convection is narrowed and enhanced in the large-scale updraft of the Hadley cell; (2) the latitudinal amplitude of Hadley cell updraft oscillations is decreased; and (3) a time lag is introduced. These effects are observable in the location and timing of convective methane clouds in Titan’s atmosphere as a function of season. A comparison of simulations over a range of convective regimes with available observations suggest methane thermodynamic-dynamic feedback is important in the Titan climate.