Bacterial and Virus‐Like Particle Abundances in Purged and Unpurged Groundwater Depth Profiles

Bacteria and viruses are ubiquitous in subterranean aquatic habitats. Bacterial abundance is known to vary with depth in aquifers; however, whether viral abundance varies with depth is less well known. Here we use flow cytometry (FCM) to enumerate bacteria and virus‐like particles (VLP) from groundwater depth profiles. Groundwater samples were obtained from a set of nested piezometers from depths of 15, 30, 45, 60, 80, and 90 m and bacteria and VLP abundances were determined in purged aquifer water and unpurged water at each slot depth. Mean bacterial abundance (cells / mL) was not significantly different in unpurged water (3.2 × 10⁵) compared to purged water (1.4 × 10⁵); however, mean VLP abundance (particles / mL) was significantly greater in unpurged water (4.4 × 10⁵) compared to purged water (2.3 × 10⁵). Purged water was used to investigate the aquifer depth profile and bacterial and VLP abundances were observed to vary significantly between depths. The virus‐bacteria ratio was determined and was observed to steadily increase with depth. Overall, our data indicate the dynamic nature of bacterial and viral abundances in subsurface environments which should be considered when designing groundwater microbial sampling methodologies.     

Potential implications of differential preservation of testate amoeba shells for paleoenvironmental reconstruction in peatlands

Testate amoebae are now commonly used in paleoenvironmental studies but little is known of their taphonomy. There is some experimental evidence for differential preservation of some testate amoeba shell types over others, but it is unclear what, if any impact this has on palaeoenvironmental reconstruction. To investigate this issue we looked at palaeoecological evidence for the preservation of different shell types. We then investigated the possible impact of selective preservation on quantitative palaeoenvironmental inference. We first used existing palaeoecological data sets to assess the vertical patterns of relative abundance in four testate amoeba shell types: (1) shells made of secreted biosilica plates (idiosomes, e.g. Euglypha), (2) idiosomes with thick organic coating (Assulina), (3) proteinaceous shells (e.g. Hyalosphenia), (4) shells built from recycled organic or mineral particles (xenosomes) (e.g. Difflugia, Centropyxis). In three diagrams a clear pattern of decay was only observed for the idiosome type. In order to assess the implications of differential preservation of testate amoeba taxa for paleoenvironmental reconstruction we then carried out simulations using three existing transfer functions and a wide range of scenarios, downweighting different test categories to represent the impact of selective test decomposition. Simulation results showed that downweighting generally reduced overall model performance. However downweighting a shell type only produced a consistent directional bias in inferred water table depth where that shell type is both dominant and shows a clear preference along the ecological gradient. Applying a scenario derived from previous experimental work did not lead to significant difference in inferred water table. Our results show that differential shell preservation has little impact on paleohydrological reconstruction from Sphagnum-dominated peatlands. By contrast, for the minerotrophic peatlands data-set loss of idiosome tests leads to consistent underestimation of water table depth. However there are few studies from fens and it is possible that idiosome tests are not always dominant, and/or that differential decomposition is less marked than in Sphagnum peatlands. Further work is clearly needed to assess the potential of testate amoebae for paleoecological studies of minerotrophic peatlands.     

长白山区典型泥炭藓沼泽的有壳变形虫

选择长白山区哈泥、金川、圆池和赤池4个典型泥炭藓沼泽作为研究地点,在系统采样基础上调查了有壳变形虫的种类组成特征.75个样品共记录到有壳变形虫33种.这些种类均为国外研究中的常见种类,其中优势种类有苔藓鳞盖虫(Assulina muscorum)、表壳圆壳虫(Cyclopyxis arcelloides type)、蛹壳虫/三足虫(Croythion-Trinema type)、巧茄壳虫(Hyalosphenia eleglans)、结节鳞壳虫(Euglypha tuberculata type)、网匣壳虫(Centropyxis cassis type)、染色梨壳虫(Nebela tincta)、泥炭藓截口虫(Heleopera sphagni).特别是凤蝶茄壳虫(H.papilio)为国内新纪录,它是泥炭藓沼泽的典型种类之一.其他研究中常见的Amphitrema flavum在本研究中未发现.     

Testate amoeba analysis of lake sediments: impact of filter size and total count on estimates of density,species richness and assemblage structure

Testate amoebae are informative about palaeoecological conditions, but the methods generally used for their analyses in lake sediments differ from those used for their analyses in peats, making comparisons difficult. This study examines how filter mesh size and total number of individuals counted affect species richness, Shannon diversity, equitability, density and assemblage structure. We analysed the complete testate amoeba contents of six sediment samples from Lake Lautrey, France. The abundance of testate amoebae was high (1,403–10,870 shells cm⁻³), and species smaller than 63 μm in both length and width represented up to 89% of total abundance and 43% of species richness. A simulation showed that using 47- or 63-μm mesh-size filters reduced inter-sample differences and changed the patterns of abundance, species richness and assemblage structure, causing loss of information and leading to potential erroneous palaeoecological interpretation. Rarefaction analyses suggest that although 170 shells are sufficient to assess the general structure of assemblages, such small sample sizes can underestimate species richness by overlooking taxa with relative abundances <4%. Total counts of 400 shells yield better estimates of assemblage structure and recover at least 50% of total species richness, although species with absolute frequencies below 2% may still be missed. Higher counts are required to obtain reliable estimates of species richness and assemblage structure in samples that have high testate amoeba densities but are dominated by a few small taxa. Further studies should determine the bioindicator value and functional roles of small and/or rare species in lakes and thus to what extent overlooking them affects palaeoecological interpretations.     

Compact,concurrent, and contiguous: smart growth and 50 years of residential planning in the Toronto region

In this article, we use parcel-based land-use data to analyze 50 years of residential development in the Toronto region. We test two hypotheses: (1) Toronto’s form does not conform to conventional definitions of suburban sprawl and (2) Toronto’s suburban development shows high levels of continuity over time with relatively high densities and mixed housing types. Contrary to recent research suggesting a convergence of urban forms among North American metropolitan regions, Ontario’s robust planning system has created a distinctive, highly consistent pattern of residential development that has, for half a century, achieved many of the core goals of smart growth including relatively compact, contiguous, and concurrent development. This form continues to be automobile dependent, however, and is not producing many of the benefits ascribed to smart growth. Rather than continuing to adopt United States-inspired smart growth policies, a more ambitious set of initiatives will be required to address current regional challenges.     

GPS tomography in the polar cap: comparison with ionosondes and in situ spacecraft data

Tomographic 4D reconstructions of ionospheric anomalies appearing in the high-latitude polar cap region are compared with plasma density measurements by digital ionosonde located near the north magnetic pole at Eureka station and with in situ plasma measurements on-board DMSP spacecraft. The moderate magnetic storm of 14–17 October 2002 is taken as an example of a geomagnetic disturbance which generates large-scale ionospheric plasma anomalies at mid-latitudes and in the polar cap region. Comparison of the GPS tomographic reconstructions over Eureka station with the ionosonde measurements of the F layer peak densities indicates that the GPS tomography correctly predicts the time of arrival and passage of the ionospheric tongue of ionization over the magnetic pole area, although the tomographic technique appears to under-estimate the value of F peak plasma density. Comparison with the in situ plasma measurements by the DMSP SSIES instruments shows that the GPS tomography correctly reproduces the large-scale spatial structure of ionospheric anomalies over a wide range of latitudes from mid-latitudes to the high-latitude polar cap region, though the tomographic reconstructions tend to over-estimate the density of the topside ionosphere at 840 km DMSP orbit. This study is essential for understanding the quality and limitations of the tomographic reconstruction techniques, particularly in high-latitude regions where GPS TEC measurements and other ionospheric data sources are limited.     

Late Holocene Neoglacial conditions from the Lesotho highlands, southern Africa: phytolith and stable carbon isotope evidence from the archaeological site of Likoaeng

The late Holocene environmental history of the Lesotho highlands, southern Africa, is poorly understood with few detailed studies to date. At Likoaeng, Senqu Valley, Lesotho, a 3 m stratified sedimentary sequence from an open-air archaeological site records vegetation development for the period 3400-1070 cal. BP. Phytolith analyses and bulk sediment organic matter δ¹³C indicate that C₄ grassland dominated the lower part of the sequence until approximately 2960 cal. BP when there was a switch to C₃ Pooid grassland (2960-2160 cal. BP). Also noted was a change from hunting mainly bovids to a dominance of fishing at the site. The change in grassland type and archaeological subsistence strategies corresponds with an episode of neoglacial cooling and the expansion of Alpine sourgrasses into lower altitudes. From 2160 to 1600 cal. BP grassland became a mix of C₃ and C₄ types and by 1600-1070 cal. BP there was a return to C₄ dominated grassland. During this latter phase there was a reversal from fishing to hunting again (and eventually some keeping of domestic livestock) at the site. These data outline the vegetation response to latitudinal shifts of frontal systems, and relatively strong atmospheric circulation variability, perhaps underpinned by variations of polar water into the Benguela Current during the late Holocene.