Seasonal variation in lysosomal destabilization in oysters,Crassostrea virginica

Lysosomal destabilization assays have been used as valuable biomarkers of pollutant exposures in a variety of bivalve and fish species. The responses of oysters, Crassostrea virginica, deployed at and native to various reference and degraded sites were evaluated for lysosomal destabilization during both summer and winter seasons. In both native and deployed oysters, lysosomal destabilization rates tended to be higher during the winter at both reference and polluted sites. There are at least two hypothetical explanations. Greater lysosomal destabilization rates may be related to physiological changes associated with mobilization of nutrient reserves during the winter and gametogenesis. However, lysosomal destabilization in deployed oysters was correlated with tissue metal concentrations. These data also support a second hypothesis that seasonal differences in physico-chemical factors (such as reduced levels of acid volatile sulfides) may increase the bioavailability of metals during the winter so that adverse effects are more pronounced.     

盛夏南海台风的结构分析

本文通过对南海台风的结构分析,得到如下几点结果: 1.在低压、台风时期,对流层中上层均具有明显的暖心结构,温度距平值发展期达最大,暖心核高度,后者高于前者。湿度中心位于700hPa。 2.台风范围内对流层的高层和低层分别存在东北风和西南风大值区,东风和南风气流对台风的发生发展起重要作用。 3.南海台风各物理量的水平分布不对称,散度具有10~(-5)S~(-1)的相同量级。涡度随气旋的发展而增强,扰动阶段比强盛阶段中心附近的涡度值约小一个量级。 4.垂直运动随着台风的加深,量级逐渐增大,极值高度升高,在强盛期达到最大值,而且它位于300hPa,垂直运动极值及其所在高度的这种变化在很大程度上反映了积云对流活动的强弱。 5.通过与西太平洋台风的比较,认为对流层高、低层的涡度差和散度差与南海台风发生发展的关系密切。     

渤海三维潮流数值模拟

本模型以具有自由面的三维非线性Ｎａｖｉｅｒ－Ｓｒｏｋｅｓ方程为基本方程，经σ坐标变换得到模式方程。基于过程分裂法，将三维流动中的快过程与慢过程劈开，并对每部分选用适合其物理特性和数值行为的计算格式，然后耦联求解。应用本模型详细计算了渤海的三维潮运动。其中，潮波系统模拟获得理想的结果；采用十层模式计算了Ｍ２潮流，以较高的辨率揭示了潮流的空间结构特征。最后本文给出了三维潮流预报的应用实例。     

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.     

近海工程环境应用中各种风资料的平均时间分析

本文从近海工程设计应用的需要出发，对于通常使用的不同来源的测风资料平均时间进行了分析，这些资料来源包括：岸边及近海探查与生产设施上的气象台站、天气观测船、浮标、商船气象报、从受灾情况估计出的极大风速以及动力诊断模型估计出的海面风速。     

等鞭金藻8701培养液的营养组成研究

等鞭金藻８７０１品系是作用（周，孙）新分离的一个单细饵料藻种。其形态构造与绿光等鞭金藻相同，便适温远较后者为低。通过单因子和正交试验表明，培养液的最佳配方是在天然海水中加入ＮａＮＯ３３０ｐｐｍ，ＣＯ（ＨＮ２）２１５ｐｐｍ，ＫＨ２ＰＯ４或ＮａＨ２ＰＯ４－８ｐｐｍ，ＦｅＣ６Ｈ５Ｏ７０．５ｐｐｍ，维生素Ｂ１０．１ｐｐｍ和维生素Ｂ１２０．０００５ｐｐｍ。