Effect of Groundwater Fluctuations on Pore Pressures and Earth Pressures on Coastal Excavation Retaining Walls

Pore water and earth pressures acting on retaining structures are investigated using an efficient coastal double-layered excavation model to determine offshore excavation responses to groundwater fluctuations outside foundation pits. Total pore water pressure includes excess pore water pressure (due to groundwater fluctuations) and steady pore water pressure (due to steady seepage) determined using one-dimensional consolidation theory of double-layered soil and one-dimensional steady-state flow theory, respectively. Rankine's active and passive earth pressures are obtained from pore water pressure. This method is applicable to arbitrary groundwater fluctuation conditions. How physical parameters affect pore water pressure is numerically investigated using examples, demonstrating the method's practicality for calculating pore water and earth pressures.     

深海细菌太平洋火色杆菌中α-淀粉酶基因amy608的克隆表达及其酶学性质研究

从来自深海沉积物的太平洋火色杆菌(Flammeovirga pacifica)的基因组中发现了一个全长为1 875 bp的α-淀粉酶基因amy608.该基因在数据库中找不到具有同源性的序列,而所编码的Amy608蛋白与已注册蛋白的氨基酸序列相似性最高仅为56%,但具有α-淀粉酶水解活性所必需的保守基序DXEXD.进化树分析表明其属于糖苷水解酶13(GH13)家族第二亚家族.构建了p ColdΙ-amy608表达载体,在大肠杆菌中进行重组Amy608蛋白的异源表达,并采用镍离子亲和层析柱对重组蛋白进行了纯化.酶学性质分析表明,重组酶Amy608的最适作用温度为40℃,在40℃保温4 d后,仍保留70%以上的酶活,显示出良好的中温热稳定性;最适p H值为7.0,p H值范围在6~9时仍保留60%以上的酶活力,表明该酶具有较宽的p H值作用范围.Ca2+、Na+、K+对α-淀粉酶Amy608有激活作用,尤其是Ca2+可使酶活显著提高40%.薄层色谱分析结果显示该酶水解可溶性淀粉的最终产物以葡萄糖为主,表明该酶是一个糖化型淀粉水解内切酶.这些结果表明Amy608为GH13家族第二亚家族中一个新型的α-淀粉酶.     

Involvement of cyclic electron flow in irradiance stress responding and its potential regulation of the mechanisms in <Emphasis Type="Italic">Pyropia yezoensis</Emphasis>

Pyropia yezoensis, belongs to the genus of Porphyra before 2011, inhabit on intertidal zone rocks where irradiation changes dramatically, implying that the seaweed has gained certain mechanisms to survive a harsh environment. Based on the photosynthetic parameters with or without the inhibitors determined by a Dual-PAM-100 apparatus, we investigated the photosynthetic performance and the changes in electron flow that occurred during the algae were stressed with different light intensities previously. When the irradiation saturation was approaching, the CEF around PS I became crucial since the addition of inhibitors usually led to an increase in non-photochemical quenching. The inhibitor experiments showed that there were at least three different CEF pathways in Py. yezoensis and these pathways compensated each other. In addition to maintaining a proper ratio of ATP/NAD(P)H to support efficient photosynthesis, the potential roles of CEF might also include the regulation of different photoprotective mechanisms in Py. yezoensis. Under the regulation of CEF, chlororespiration is thought to transport electrons from the reduced plastoquinone (PQ) pool to oxygen in order to mitigate the reduction in the electron transfer chain. When irradiation was up to the high-grade stress conditions, the relative value of CEF began to decrease, which implied that the NADP⁺ pool or PQ⁺ pool was very small and that the electrons were transferred from reduced PS I to oxygen. The scavenging enzymes might be activated and the water-water cycle probably became an effective means of removing the active oxygen produced by the irradiation stressed Py. yezoensis. We believe that the different mechanisms could make up the photoprotective network to allow Py. yezoensis for survival in a highly variable light stress habitat, which may enlighten scientists in future studies on irradiance stress in other algae species.     

40Ar‐39Ar Dating of the Mahuaping W‐Be deposit,Northwestern Yunnnan Province,China, and its Geological Significance

正Objective Located near the intersection of Yangtze craton,TibetSanjiang orogen and Songpan-Ganzi orogen of Northwest Yunnan Province,the Mahuaping deposit is a unique large-scale tungsten-beryllium-fluorite ore deposit ever discovered in this area.In recent years,many studies have been carried out in Sanjiang Tethyan metallogenic belt and     

包气带反硝化强度空间分布规律的整合分析

依据1970~2017年间发表的3955篇包气带反硝化强度相关论文,筛选出197组反硝化强度数据,利用整合分析,重点研究了包气带反硝化强度在典型生态类型（水平）和不同采样深度（垂向）的空间分布规律,识别了包气带反硝化强度的主控因素并研究其函数关系。结果表明:水平空间上,包气带表层0~0.5 m反硝化强度的分布特征显著,由大到小排序为:森林（8.03±0.21 mg/（kg·d））、农田（3.54±0.08 mg/（kg·d））、草地（3.38±0.12 mg/（kg·d））、湿地（2.32±0.23 mg/（kg·d））、沙漠（2.15±0.56 mg/（kg·d））。垂向空间上（6 m内）,各生态类型反硝化强度随深度的增加均呈“S”型变化规律。不同生态类型和不同采样深度下包气带反硝化强度的主控因素存在一定差异,主要为黏粒、有机质、全氮、硝态氮、有效磷,并给出了包气带反硝化强度与主控因素的回归方程。     

崩塌堆积体启动坡面泥石流判别与防治 ——以北川县开坪乡平石板泥石流为例

强震区地震后大量的崩塌堆积体堆积于高陡斜坡中上部,堆积体远离居民点,一旦诱发为坡面泥石流,形成新的次生地质灾害,将严重威胁下方居民的生命财产安全。本文以北川县开坪乡平石板坡面泥石流为例,平石板沟“5.12”地震崩塌堆积体在2010、2013年强降雨作用下失稳转变为坡面泥石流淤积场镇。在平石板泥石流暴发特征介绍基础上,通过粒径不均匀系数、堆积体启动判别指标、后缘汇水面积等来分析崩塌堆积体启动条件及影响因素,并针对此类灾害发育特征,提出了通过上游稳拦固源方法进行治理的防治思路。以期为研究高陡斜坡堆积体的启动机制及防治提供一定的借鉴意义。     

基于斜坡破坏模式的软弱变质岩区岩质滑坡易发性评价

滑坡受控于地质环境条件和坡体地质结构,不同地质结构的斜坡失稳破坏的模式存在差异,本文以后龙门山千枚岩区为例,基于现场调查,梳理总结了区内岩质滑坡发生的力学模式与斜坡地质环境、坡体结构之间的关系,进而选取控制滑坡发生的关键因素,运用ARCGIS软件的加权叠加功能定量划分了不同模式滑坡的易发区域,主要取得以下认识：（1）滑移—拉裂式滑坡主要发生在坡度30-45°的顺向飘倾坡内、滑移—溃曲式滑坡发生在坡度30-45°的顺向伏倾坡内、倾倒变形主要发生在岩层倾角大于65°的反向斜坡内;（2）区内倾倒变形易发区面积为8.73km2、滑移拉裂易发区面积为4.31m2、滑移溃曲易发区面积为3.28km2,以倾倒变形类型的滑坡为主,已发生滑坡与易发分区结果比对证明了滑坡易发分区与实际情况基本吻合。     

The Middle Triassic evolution of the Bangong–Nujiang Tethyan Ocean: evidence from analyses of OIB-type basalts and OIB-derived phonolites in northern Tibet

International Journal of Earth Sciences - In this paper, we present new major and trace element chemical data for the basalts and phonolites of the Nare ocean island fragment (NaOI), as well as...     

A robust mesh optimisation method for multiphase porous media flows

Flows of multiple fluid phases are common in many subsurface reservoirs. Numerical simulation of these flows can be challenging and computationally expensive. Dynamic adaptive mesh optimisation and related approaches, such as adaptive grid refinement can increase solution accuracy at reduced computational cost. However, in models or parts of the model domain, where the local Courant number is large, the solution may propagate beyond the region in which the mesh is refined, resulting in reduced solution accuracy, which can never be recovered. A methodology is presented here to modify the mesh within the non-linear solver. The method allows efficient application of dynamic mesh adaptivity techniques even with high Courant numbers. These high Courant numbers may not be desired but a consequence of the heterogeneity of the domain. Therefore, the method presented can be considered as a more robust and accurate version of the standard dynamic mesh adaptivity techniques.