Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

Design and implementation of web fast sharing platform of massive geological map

It is still a laborious work to consult massive multidisciplinary geological maps which are scattered in different geological departments,and hard to give full play to the potential application value of geological map. Along with the network,the development of communication and computer technology,especially with the Web2.0 technology arisen,provides opportunities to the web for sharing of geological maps. Web fast sharing platform of massive geological maps( WSPGM) is based on the Flex technology development,using B/S architecture,with map navigation,search,display,security and other functions of the software system. Through the map pretreatment and asynchronous communication the system can display any size of geological map,and through the range limits,rights management,sharing system and watermark logo to take effective security on geological map. On the basis of this system,with the Jilin University web sharing system of geological map,the system can greatly improve the efficiency of using geological map. for research.     

电离层E_S层对综合射束污染的初步分析

本文考虑到干涉仪对电离层中不同尺度不规则性的滤波作用、结合密云米波综合孔径系统、提出了电离层E_(?)层是电离层不规则性影响该系统的主要根源。不规则性的尺度约在700～1000米的范围。再考虑到E_(?)层的漂移运动,提出了文中所描述的模型。用电子计算机进行了E_(?)层对综合射束污染的模拟计算,结果指出:射束主瓣的增益下降约10％,副瓣由13％增大到18％;综合射束在赤经方向出现不对称性,从而引起约0.6秒的赤经误差;射束的边缘处和两个扇形区出现了杂瓣。     

Radio variability of the blazar S5 0716+714: a ∼6.1 year quasi-periodicity

We report a ～6.1 yr quasi-periodicity for the blazar S5 0716+714 using the radio light curves at 4.8, 8 and 14.5 GHz observed by the University of Michigan Radio Astronomical Observatory (UMRAO) from 1981 to 2012, by means of the Jurkevich, discrete correlation function (DCF) and power spectral analysis techniques. There are a general correlation among light curves at different frequencies and a time lag of \(170\pm 10\) days between 4.8 and 14.5 GHz light curves can be confirmed. We also estimate the orbit parameters assuming a binary black hole system, and the magnetic field strength under the jet comoving frame.     

GPS掩星技术低轨卫星计划的现状及进展

主要介绍了几个国际上已经实施的GPS掩星计划（GPS／MET，Φrsted,Sunsat,SAC-C,CHAMP)和在研的（COSMIC，ACE）低轨卫星计划的现状及取得的进展。通过总结可知，下一代GPS掩星接收器应具有体积小、质量轻（几百克）、低功耗的特点；能提供实时的卫星物理状态参数；飞行器上的自主计算和控制；星上数据通信和命令解释；所有跟踪数据应符合厘米级精密定轨的要求；能提供掩星和海洋反射实验的测量。     

湖相深水细粒物质的混合沉积作用探讨*

沉积物的混合是自然界的普遍现象,湖盆中广泛发育的深水细粒沉积岩本身就是一类混积岩。以东营凹陷古近系沙河街组三段下亚段—沙河街组四段上亚段细粒沉积岩为研究对象,通过厘米级岩心观察描述、小型沉积构造解剖、岩石薄片观察、X射线衍射全岩矿物分析及微量元素测试等方法,研究细粒物质的产出状态和混合方式;分析湖盆流体、气候及水体性质对细粒物质迁移、混合、沉积的控制作用,尝试探讨细粒物质的混合沉积机制。结果表明,湖相深水细粒沉积岩既有物质成分上的混合,也有结构的混合,且在宏观与微观的不同尺度均存在混合;混合沉积作用有均匀混合、纹层叠置混合、不均匀团块状混合等类型。纹层叠置混合沉积是最主要的混合沉积方式,依据纹层接触关系、所占比例及矿物产出状态可分为泥砂—灰纹层叠置混合型、泥—灰纹层叠置混合型、泥砂粒序—灰纹层叠置混合型、灰—泥—云纹层叠置混合型等。湖相深水细粒物质的混合沉积作用类型多且往往受多个因素同时控制,气候及水动力条件是控制细粒混合沉积岩发育的最主要因素,不同流体(浊流和底流)作用下的细粒沉积物混合作用存在差异。气候引起湖水性质变化及水体分层,从而控制不同类型细粒物质的絮凝沉降及化学沉积。不同构造部位、不同水体深度的混合作用不同;相同构造部位在垂向上也存在混合沉积方式的差异。     

江西九瑞矿集区硅质断裂磨砾岩带的厘定及其成岩成矿意义

在九瑞矿集区研究叠合断裂和叠加成矿作用的基础上,我们进一步详细研究了出露在洋鸡山-丁家山-望夫山一线的硅质角砾岩,指出它们不是原先认为的石炭系沉积硅质岩,而应属于一种断裂磨砾岩,并深入探究其形成过程及与成矿之关系.断裂磨砾岩是断裂分带结构成熟的标志之一,多在剪切作用和热液作用下,断裂岩石经硅化-破裂-碎裂-粉碎-研磨,形成具有一定圆度和球度,大小差异较大的磨砾或磨粒,且又会反复的集结-破碎,不断拓宽断裂构造形成磨砾-角砾岩带.本文研究的断裂磨砾岩,呈北东向展布,延长达十几千米.成分上以硅化角砾岩为主,SiO2含量一般大于90％,石英颗粒由隐晶到显晶.一些角砾岩中含Fe2O3较高,有可能是原先的硫化物经氧化形成的褐铁矿.本区洋鸡山-丁家山-望夫山一线产出的断裂磨砾-角砾岩带,很可能是燕山期构造-岩浆-成矿事件的产物.在城门山和武山铜矿,我们之前的工作己发现存在产于泥盆系五通组和石炭系黄龙组层滑构造体系中的黄铁矿角砾岩,则有可能属于海西期同生断裂活动的产物.因此,这些不同的角砾岩具有多阶段活动和叠加成矿的特征.本文还进一步指出,九瑞地区其他层位(如泥盆系与志留系之间、志留系与奥陶系之间)发育的层滑构造体系和断裂角砾岩及热液蚀变岩,也很可能是成矿有利部位,值得今后找矿工作的关注.     

A New Feature Vector Using Selected Line Spectra for Pulsar Signal Bispectrum Characteristic Analysis and Recognition

Average pulse profiles of pulsar signals are analyzed using the bispectrum tech-nique. The result shows that there are nonlinear phase couplings between the two frequency axes of the bispectrum charts, which indicate nonlinear factors in the generation and prop-agation of pulsar signals. Bispectra can be used as feature vectors of pulsar signals because of their being translation invariant. A one-dimension selected line spectrum algorithm for ex-tracting pulsar signal characteristic is proposed. Compared with selected bispectra, the pro-posed selected line spectra have the maximum interclass separability measurements from the point of view of the whole one-dimension feature vector. Recognition experiments on several pulsar signals received at several frequency bands are carried out. The result shows that the selected line spectrum algorithm is suitable for extracting pulsar signal characteristics and has a good classification performance.     

Isolation and pharmacological activities of bromophenols from Rhodomela confervoides

Four bromophenols were isolated from the extract of marine red alga,Rhodomela confervoides by column chromatography and HPLC methods. By means of spectroscopic methods inclding IR, MS, 1D, and 2D-NMR techniques, their structures were elucidated as (1) 3-bromo-4,5-dihydroxy benzoic acid methyl ester; (2) bis (2,3-dibromo-4,5-dihydroxybenzyl) ether; (3) 3,4-dibromo-5-(methoxymethyl)-1,2-benzenediol and (4) 3-bromo-4,5-dihydroxy-benzaldehyde. Compound 1 was first isolated from the algae in nature, and 1, 4 were found to have selective cytotoxic activities against KB, Bel 7402 and A549 cells, 2 showed powerful antibacterial activities againstStaphylococcus aurens andPseudomonas aeruginosa. Supported by National “863” Program (No. 2004AA625030, 2001AA620503), Quingdao marine sciences project (No. 04-2-NN-26) and Key Knowledge Innovative Project of the Chinese Academy of Sciences (KZCX3-SW-215).