Esterases as biomarkers in Nereis (Hediste) diversicolor exposed to temephos and Bacillus thuringiensis var. israelensis used for mosquito control in coastal wetlands of Morbihan (Brittany,France)

Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins.     

俄罗斯沿岸更新世盆地类型

俄罗斯沿岸地区受到分别属于大西洋、北冰洋和太平洋的14个海的海水冲刷，海岸线的总长度超过60000km。在更新世，这些盆地经历过不止一次的幅度不同的海平面的升降作用。在海退时期，广阔的陆架地区变干了，而在海侵时期，沿岸平原和低山岸区被海水淹没。在东北     

Contamination of organotin compounds and imposex in molluscs from Vancouver,Canada

Gastropods and bivalves were collected at 15 sites at Vancouver and Victoria, Canada between 24 May and 7 June, 1999, to establish tissue concentrations of butyltin and phenyltin compounds, to record imposex symptoms in gastropods, and to assess the present status of organotin contamination around Vancouver. No neogastropods (such as Nucella lima) were found around Vancouver. Neogastropod populations could have been extirpated by severe TBT contamination in Vancouver, as relatively high concentrations of TBT were detected in tissues of Mytilus trossulus from Vancouver, and the neogastropods distributed in Vancouver might be sensitive to TBT. Recovery from imposex, however, was observed in neogastropod populations from three sites at Victoria and Mission Point. TBT contamination has continued around Vancouver, arising from continuous use of TBT in antifouling paints for vessels larger than 25 m in length; however, TBT has decreased around Victoria and Mission Point. Different patterns of TBT accumulation in tissue were observed among the bivalve species from Vancouver. The highest TBT concentration detected in Tresus capax suggested some possible adverse effects. TBT was the most predominant butyltin component in almost all bivalve specimens surveyed, suggesting a low rate of TBT metabolism. Phenyltin compounds were not detected in any molluscan specimens in this study.     

Development of two novel CYP-antibodies and their use in a PCB exposure experiment with Mytilus edulis

In an attempt to learn more about the cytochrome P450 (CYP) system of mussels, we used protein databases and alignment software to extract highly conserved CYP sequences. From these alignments synthetic peptides were produced and used for rabbit immunisation, which yielded polyclonal antibodies against the CYP families 2 and 4. The antibodies were evaluated with Western Blot and ELISA assays, using digestive gland microsomal samples from the mussel Mytilus edulis. Western Blots revealed immunoreactions for both antibodies. The anti-CYP2 sequence rendered one major immunopositive protein of approximately 49 kDa size, and weak signals for proteins of approximately 41 and 56 kDa size. The anti-CYP4 sequence rendered two major bands of approximately 56 and 59 kDa size, and also a weak immunoreaction with a protein of approximately 43 kDa size. ELISA rendered only weak signals even with a 1:50 dilution of IgG-purified serum. A 10-day exposure to Aroclor 1254 did not appear to affect any of the immunopositive proteins, while total PCBs in soft bodies increased from 14-40 ng/g DW in controls to 373-638 ng/g DW in exposed mussels.     

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。     

通过对重力波曳力的参数化来减小数值天气预报和大气环流模式中的系统性误差

一、引 言 由于地形性激发的中尺度重力波的中断,在加拿大气候中心大气环流谱模式中有时候采用了波的曳力效应的参数化。这一参数化的细节以及它在大气环流模式气候模拟中影响的讨论,可见Boer等人(1985)的工作。 最近,人们利用激发重力波次尺度地形的更好的描绘,来修改这一参数化方案。还有,使用Lindzen(1981)的波饱和概念来描述曳力效应。这一关于地形重力波曳力参数化的作法已在加拿大气候中心的大气环流谱模式中广泛试验,在加拿大气象中心的业务的谱数值预报模式中也正在普遍进行试验。本文的目的所在于概述在这些模式中正在普遍使用的波的曳力方案,并且证明这一方案在消除中期预报和大气环流模拟的系统性误差方面的用处。     

海南岛珊瑚礁的主要类型

一、前言 中苏海洋生物考察团,于1958年3—6月在中国南海的海南岛进行了考察工作,对该地区的海洋潮间带动物群的栖息条件和沿岸的动物生态学进行了调查研究。工作中除考察了岩石、红树丛、沙滩及泥滩的生物群落外,同时考察了热带海洋中典型的生物群落——珊瑚礁。     

黄海多毛类动物地理学的初步研究

1.黄海多毛类区系包含有中国沿岸特有地方性种,黄海和日本沿岸的特有地方性种,广温性热带暖水种,寒温带冷水种和广布种等不同性质成分。 2.值得提出的是太平洋东西两岸种在黄海的发现,计有19种。这些种的分布应该考虑不仅是由于历史的成因,在某种程度上幼体也许有可能随当前自日本沿岸流向美洲沿 岸的黑潮传布过去。多毛类有些种幼虫期很长,并且能随海流分布至深海(Thorson,1950)。 3.根据与邻近海区区系的比较,黄海多毛类与日本沿岸,特别是日本本州北部金华山附近的女川湾和松岛湾一带的区系最相近似,女川湾和松岛湾一带属亲潮寒流和黑潮暖流交汇区,因之在种类组成上不仅有来自南方的暖水种,同时,还有北方的冷水种,具有混合区系特征。黄海——本州北部应属同一地理区划。 4.黄海多毛类具有十分显著的混合区系特征,与日本的情况不同,黄海的暖水种多分布在潮间带,冷水种大部分在较深水域,井与深水区终年存在的冷水团有关。 5.黄海北部和中部应属北太平洋北温带和印度西太平洋热带区之间的过渡区。     

Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice

The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis.