Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

Marine mammals from northeast Atlantic: relationship between their trophic status as determined by delta13C and delta15N measurements and their trace metal concentrations

The relationship between trophic position through delta13C and delta15N and trace metal concentrations (Zn, Cd, Cu and Hg) was investigated in the tissues of six marine mammal species from the Northeast Atlantic: striped dolphin Stenella coeruleoalba, common dolphin, Delphinus delphis, Atlantic white-sided dolphin Lagenorhynchus acutus, harbour porpoise Phocoena phocoena, white beaked-dolphin Lagenorhynchus albirostris, grey seal Halichoerus grypus stranded on French Channel and Irish coasts. White-beaked dolphins, harbour porpoises, white-sided dolphins, common and striped dolphins display the same relative and decreasing trophic position, as measured by delta15N values, along both the Irish and French channel coasts, reflecting conservative trophic habits between these two places. Hepatic and renal Cd concentrations were significantly correlated to muscle delta13C and delta15N values while Hg, Zn and Cu did not. These results suggest that Cd accumulation is partly linked to the diet while other factors such as age or body condition might explain Hg, Zn or Cu variability in marine mammals. Combined stable isotope and trace metal analyses appear to be useful tools for the study of marine mammal ecology.     

Nonlinear response of a moored buoy

The nonlinear dynamic analysis of a multipoint slack moored buoy is performed under the action of first and second order wave forces. The nonlinearity of the system is caused by the geometric nonlinearity of the mooring lines. The resulting nonlinear equation of motion is solved by an incremental time marching scheme. The nonlinear responses of the system are analysed to investigate different kinds of dynamic instability phenomena that may arise due to the nonlinearity of the system. As an illustrative example, a hollow cylindrical buoy anchored to the sea bed by means of six slack mooring lines is considered. The responses of the system are obtained and analysed for three regular waves namely, 5 m/5 s, 12 m/10 s and 18 m/15 s. The results of the study show that different kinds of instability phenomena like nT subharmonic oscillations, symmetry breaking bifurcation and aperiodic responses may occur in slack mooring systems. Further, a second order wave force may considerably influence the dynamic stability of such systems.     

An alternative approach to the structural motion analysis of wedge-beam offshore structures supporting a load

For convenience of dynamic analysis, some offshore structures such as fixed-type platforms are often modeled as the wedge beams supporting tip lumped masses. It is well-known that, due to the effect of the surrounding water, the natural frequencies of a beam in air (or dry beam) are different from those of the same beam immersed in water (or wet beam). However, if the natural frequencies and the associated mode shapes of a dry beam are calculated by taking account of the “added mass” for the immersed beam, then the last natural frequencies and mode shapes will be equal to the corresponding ones of the wet beam. Based on the last concept, the closed form solutions for natural frequencies and the associated mode shapes of the dry beam were determined first, then the partial differential equation of motion for the wet beam was transformed into a matrix equation by using the expansion theorem and the foregoing closed form solutions of free vibration responses for the dry beam. Solving the last matrix equation will give the required natural frequencies and the associated mode shapes of the wet beam. The formulation of this paper is available for the fully or partially immersed double tapered beams with either circular, square or rectangular cross-sections. The taper ratio for width and that for depth may be equal or unequal. The numerical results of this paper were compared with the existing results or the finite-element-method results and good agreement was achieved.     

Robust adaptive control of underactuated ships on a linear course with comfort

This paper addresses an important problem in ship control application—the robust stabilization of underactuated ships on a linear course with comfort. Specifically, we develop a multivariable controller to stabilize ocean surface ships without a sway actuator on a linear course and to reduce roll and pitch simultaneously. The controller adapts to unknown parameters of the ship and constant environmental disturbances induced by wave, ocean current and wind. It is also robust to time-varying environmental disturbances, time-varying change in ship parameters and other motions of the ship such as surge and heave. The roll and pitch can be made arbitrarily small while the heading angle and sway are kept to be in reasonably small bounds. The controller development is based on Lyapunov’s direct method and backstepping technique. A Lipschitz continuous projection algorithm is used to update the estimate of the unknown parameters to avoid the parameters’ drift due to time-varying environmental disturbances. Simulations on a full-scale catamaran illustrate the effectiveness of our proposed controller.     

Use of Continuous Plankton Recorder information in support of marine management: applications in fisheries, environmental protection, and in the study of ecosystem response to environmental change

The Continuous Plankton Recorder (CPR) survey was conceived from the outset as a programme of applied research designed to assist the fishing industry. Its survival and continuing vigour after 70 years is a testament to its utility, which has been achieved in spite of great changes in our understanding of the marine environment and in our concerns over how to manage it. The CPR has been superseded in several respects by other technologies, such as acoustics and remote sensing, but it continues to provide unrivalled seasonal and geographic information about a wide range of zooplankton and phytoplankton taxa. The value of this coverage increases with time and provides the basis for placing recent observations into the context of long-term, large-scale variability and thus suggesting what the causes are likely to be. Information from the CPR is used extensively in judging environmental impacts and producing quality status reports (QSR); it has shown the distributions of fish stocks, which had not previously been exploited; it has pointed to the extent of ungrazed phytoplankton production in the North Atlantic, which was a vital element in establishing the importance of carbon sequestration by phytoplankton.The CPR continues to be the principal source of large-scale, long-term information about the plankton ecosystem of the North Atlantic. It has recently provided extensive information about the biodiversity of the plankton and about the distribution of introduced species. It serves as a valuable example for the design of future monitoring of the marine environment and it has been essential to the design and implementation of most North Atlantic plankton research.     

Nonlinear coupled dynamic response of offshore Spar platforms under regular sea waves

Many innovative floating offshore structures have been proposed for cost effectiveness of oil and gas exploration and production in water depths exceeding one thousand meters in recent years. One such type of platform is the offshore floating Spar platform. The Spar platform is modelled as a rigid body with six degrees-of-freedom, connected to the sea floor by multi-component catenary mooring lines, which are attached to the Spar platform at the fairleads. The response dependent stiffness matrix consists of two parts (a) the hydrostatics provide restoring force in heave, roll and pitch, (b) the mooring lines provide the restoring force which are represented here by nonlinear horizontal springs. A unidirectional regular wave model is used for computing the incident wave kinematics by Airy’s wave theory and force by Morison’s equation. The response analysis is performed in time domain to solve the dynamic behavior of the moored Spar platform as an integrated system using the iterative incremental Newmark’s Beta approach. Numerical studies are conducted for sea state conditions with and without coupling of degrees-of-freedom.     

Outer wave number spectrum and statistical distribution of apparent wave number vector

Based on the ray theory and Longuet-Higgins’s linear model of sea waves, the joint distribution of wave envelope and apparent wave number vector is established. From the joint distribution, we define a new concept, namely the outer wave number spectrum, to describe the outer characteristics of ocean waves. The analytical form of the outer wave number spectrum, the probability distributions of the apparent wave number vector and its components are then derived. The outer wave number spectrum is compared with the inner wave number spectrum for the average status of wind-wave development corresponding to a peakness factor P = 3. Discussions on the similarity and difference between the outer wave number spectrum and inner one are also presented in the paper.     

Distribution, texture and composition of modern phosphate pellets in Peru shelf muds

Textural analysis, including estimates of concentrations of authigenic phosphate pellets, were made for eight sediment cores from the Peru continental margin. Phosphatic pellets separated from these modern organic-rich sediments are black, spherical-ovoidal in shape, and in thin section often display a concentric structure around a nucleus consisting of inorganic mineral grains. Some pellets have a gray-white exterior coating which appears to be secondary diagenetic calcite. Phosphatic pellets account for upwards of 80% of the sediment mass in some cores. Pellets concentrate in specific size classes, generally between 125 and 500 μm in diameter, and occur within a poorly sorted sediment.