CYP1B1 knockdown does not alter synergistic developmental toxicity of polycyclic aromatic hydrocarbons in zebrafish (Danio rerio)

Polycyclic aromatic hydrocarbons (PAHs) are contaminants increasing in the environment largely due to burning of fossil fuels. Our previous work identified a synergistic toxicity interaction in zebrafish embryos occurring when PAHs that are agonists for the aryl hydrocarbon receptor (AHR) co-occur with PAHs that are CYP1A inhibitors. This toxicity is mediated by the AHR2, and morpholino knockdown of CYP1A exacerbated toxicity. This study tested two hypotheses: (1) in the absence of functional CYP1A, metabolism of PAHs is shunted towards CYP1B1, which has been shown in mammals to produce more reactive metabolites of PAHs; alternatively, (2) CYP1B1 serves a protective role similar to CYP1A. We used a morpholino approach to knockdown CYP1B1 alone and in co-knockdown with CYP1A to determine whether we could alter deformities caused by synergistic toxicity of PAHs. CYP1B1 knockdown was not different from non-injected controls; nor were CYP1B1+CYP1A co-knockdown deformities different from CYP1A knockdown alone. These data suggest that CYP1B1 is not a significant factor in causing synergistic toxicity of PAHs, nor, in contrast to CYP1A, in providing protection.     

Seasonal variations of exposure biomarkers, oxidative stress responses and cell damage in the clams, Tapes philippinarum, and mussels, Mytilus galloprovincialis, from Adriatic sea

This work investigated the natural variability of several biomarkers in Tapes philippinarum and Mytilus galloprovincialis, sampled from Northern Adriatic where these organisms are important sentinel species for future environmental impact assessment. Levels of metallothioneins, peroxisomal enzymes and acetylcholinesterase, showed a significant seasonality and marked differences between clams and mussels. Among antioxidant enzymes, catalase and GST decreased during the warmer period, the latter enzyme activity resulting particularly high in clams. The total oxyradical scavenging capacity toward peroxyl radicals decreased in mussels from winter to summer, indicating a prooxidant challenge due to higher seawater temperature and intensity of light irradiance. Lysosomal membrane stability did not exhibit significant seasonal variations, while some variations were observed for DNA damages. Overall results indicated a significant influence of seasonal variability on several biomarkers and species-specific differences which should be considered to discriminate the appearance of anthropogenic disturbance.     

A comparative analysis of three habitat suitability models for commercial yield estimation of Tapes philippinarum in a North Adriatic coastal lagoon (Sacca di Goro, Italy)

Habitat Suitability (HS) models have been extensively used by conservation planners to estimate the spatial distribution of threatened species and of species of commercial interest. In this work we compare three HS models for the estimation of commercial yield potential and the identification of suitable sites for Tapes philippinarum rearing in the Sacca di Goro lagoon (Italy) on the basis of six environmental factors. The habitat suitability index (HSI) is based on expert opinion while the habitat suitability conditional (HSC) is calibrated on observational data. The habitat suitability mixed (HSM) model is a two-part model combining expert knowledge and regression analysis: the first component of the model uses logistic regression to identify the areas in which clams are likely to be present; the second part applies the same parameter-specific suitability functions of the HSI model only in the areas previously identified as productive by the logistic component.The HS models were validated on an independent data set and estimates of potential yield of the Goro lagoon were compared. The effectiveness of the three approaches is then discussed in terms of predicted yield and identification of suitable sites for farming.     

Timescales and dynamics of the formation of a thermocline

In a state of equilibrium, the constraint of a balanced heat budget for the ocean strongly influences the depth of the tropical thermocline because that depth controls the rate at which the ocean absorbs heat from the atmosphere. Thus, an increase in the oceanic heat loss in high latitudes results in a shoaling of the equatorial thermocline so that the heat gain also increases. How does the ocean adjust to such a new equilibrium state after an abrupt change in the heat flux in high latitudes? The adjustment of the wind-driven circulation of the upper ocean is shown to involve two timescales. The first is the familiar adiabatic wave-adjustment time associated with the horizontal redistribution of warm water above the thermocline in shallow water models. (This is essentially the time it takes Rossby and Kelvin waves to propagate from the disturbed extra-equatorial region to the equator.) The second adjustment-time is associated with the diabatic processes that come into play once the waves from higher latitudes modify the thermal structure in low latitudes and hence the flux of heat into the ocean; it is the timescale on which the ocean recovers a balanced heat budget. The identification of this timescale is the main result of this paper.Through a series of simulations of an idealized ocean basin, we identify the diabatic timescale and argue that it is determined by the strength of the upwelling and the intensity of the air–sea heatfluxes. By simulating the formation of a thermocline from isothermal conditions, we are able to relate this timescale to other relevant timescales such as that associated with diffusive processes and the adiabatic timescale invoked by Gu and Philander [Gu, D., Philander, S.G.H., 1997. Interdecadal climate fluctuations that depend on exchanges between the tropics and extra-topics. Science 275, 805–807].     

Inclined K–Ar illite age spectra in brittle fault gouges: effects of fault reactivation and wall‐rock contamination

K–Ar clay fraction ages of brittle faults often vary with grain size, decreasing in the finer size fractions, producing an inclined age–grain‐size spectrum. K–Ar ages and mineralogical characterization of gouges from two normal faults in the Kongsberg silver mines, southern Norway, suggest that inclined spectra derived from brittle fault rocks reflect the mixing of inherited components with authigenic mineral phases. The ages of the coarsest and finest fractions constrain faulting at c. 260–270 Ma and reactivation around 200–210 Ma, respectively. This study demonstrates how wall‐rock contamination influences the K–Ar age of the coarsest size fractions and that authigenic illite and K‐feldspar can crystallize synkinematically under equivalent conditions and thus yield the same K–Ar ages.     

Direct 40Ar/39Ar dating of Late Ordovician and Silurian brittle faulting in the southwestern Norwegian Caledonides

Structural data as well as U–Pb zircon and ⁴⁰Ar/³⁹Ar biotite and muscovite ages were collected from the Rolvsnes granodiorite in western Norway. The granodiorite intruded at c. 466 Ma, cooled quickly and escaped later viscous deformation. Brittle top‐to‐the‐NNW thrust faults (Set I) and WNW–ESE striking dextral strike‐slip faults (Set II) formed in a NNW–SSE transpressional regime. ⁴⁰Ar/³⁹Ar dating of synkinematic mica from both sets reveals a c. 450 Ma (Late Ordovician) age of faulting, which constrains early‐Caledonian brittle deformation. Set I and II faults are overprinted by a set of lower‐grade, variably oriented chlorite‐ and epidote‐coated faults (Set III) constraining WNW–ESE shortening. A lamprophyric dyke oriented compatibly with this stress field intruded at c. 435 Ma (Silurian), indicating that Set III formed at the onset of the Scandian Baltica–Laurentia collision. The preservation of Caledonian brittle structures indicates that the Rolvsnes granodiorite occupied a high tectonic level throughout the Caledonian orogeny.     

Evaluation of site effects in the Aterno river valley (Central Italy) from aftershocks of the 2009 L��Aquila earthquake

A temporary network of 33 seismic stations was deployed in the area struck by the 6th April 2009, Mw 6.1 (Scognamiglio et?al. in Seism Res Lett 6/81, 2010), L??Aquila earthquake (central Italy), with the aim to investigate the site amplification within the Aterno river Valley. The seismograms of 18 earthquakes recorded by 14 of the 33 stations were used to evaluate the average horizontal to vertical spectral ratio (HVSR) for each site and the standard horizontal spectral ratio (SSR) between a site and a reference station. The obtained results have been compared to the geological and geophysical information in order to explain the resonance frequencies and the amplification levels with respect to surface geology of the valley. The results indicate that there is no uniform pattern of amplification, because of the complex geologic setting, as the thickness and degree of cementation of the deposits is highly variable.     

Diagenetic and thermal history of the Jurassic-Tertiary succession of the Zagros Mountains in the Dezful Embayment (SW Iran): constraints from fluid inclusions

To constraint the diagenetic and thermal history in the Mesozoic-Tertiary succession of the Zagros Fold-Belt, a study was performed on fluid inclusions trapped in intergranular, vug, and fracture-filling cements in Jurassic to Miocene outcrop samples collected along the Anneh and Fahliyan Valleys of Khuzestan Province, southwest Iran. Petrographic observations showed at least two systems of fractures that postdate intergranular cementation. Two different types of oil-filled fluid inclusions occur in the intergranular cements and in the first fracture network, but they are absent in the second fracture network. Microthermometry of fluid inclusions was used to determine the temperature and salinity of fluids responsible for mineral precipitation. Within intergranular cements and calcite fillings the oldest fractures, precipitation occurred from high saline fluids in a temperature range of 60–120°C, depending on the stratigraphic depth. The strong correlation between fluid inclusions temperature and stratigraphic depth suggests that the intergranular cementation and the first fracture-filling event occurred prior to structural deformation. Furthermore, this correlation indicates a relatively constant paleogeothermal gradient at the time of cement precipitation of about 28°C/Km. On the contrary, fluid inclusions trapped in cements within the second fracture network lack oil and contain freshwater trapped at low temperatures that do not correlate to the stratigraphic position. This suggests second fracturing event occurred after oil migration and during or after deformation of the Zagros Fold-Belt, when the samples were in a near-surface position.     

Flexural response of the Venetian foreland to the Southalpine tectonics along the TRANSALP profile

The Venetian Basin was affected by flexure related to the Southalpine shortening phase during the Middle Miocene – Early Pliocene. This downbending is quantified here using a two‐dimensional flexural model. A recently improved data set on basin geometry based on the bottom of the Serravallian–Tortonian clastic wedge, on palaeobathymetry and gravity anomalies is used to constrain the components of flexure and to test the importance of the initial bathymetry in evaluating the contribution of surface loads to deflection. A good fit is obtained assuming a northward broken plate configuration of the downbent Adriatic plate with an effective elastic thickness of 20 km. Results highlight that, in the studied region, flexure related to the Eastern Southern Alps is totally due to surface loads (topographic load partly replacing initial bathymetry) and that no hidden loads are required. Furthermore, the palaeobathymetry contributes up to 50% to the total flexure in the studied region.