Tracing environmental lead sources on the Ao mountain of China using lead isotopic composition and biomonitoring

Atmospheric lead(Pb) and other trace metals can transport over long distance and deposit on remote alpine ecosystems. In this work, the soil profiles, litter and dominant mosses along a large altitude were collected on Ao Mountain, Central China, to obtain the spatial distributions of Pb in these materials, decipher the possible factors controlling the distribution, and quantitatively distinguish the natural versus anthropogenic sources of Pb through the Pb isotopic tracing and biomonitoring. The results show that soil Pb concentrations(mg/kg) decreased significantly with depth, and they were markedly higher in the O(42.6± 2.7) and A(36.4 ± 2.2) horizons than in the litter(7.20 ± 1.9) and mosses(28.0 ± 3.9). The Pb enrichment in the surface soils(O and A horizons),litter and mosses existed in the relatively high altitudes, which was attributed to the influences from atmospheric wet deposition, plants, soil physicochemical properties and human activity. ThePb isotopic ratios identified the Pb sources as originating mainly from Chinese coal combustion,mining and smelting. Atmospheric Pb from southeastern, southwestern and northwestern regions could be deposited in the alpine ecosystem by long distance atmospheric transport. The anthropogenic Pb reached over 50% in the O and A horizons, and over 70% in the litter and mosses, which corresponded to the concentrations of 26.9, 17.7, 5.92 and 21.2 mg/kg, respectively. The results indicate that the mutual effects of climate and regional human activity could increase the Pb accumulation in remote alpine ecosystems.     

广州白云山常绿阔叶林的结构特征

在线路调查和样方调查的基础上分析了广州白云山常绿阔叶林的结构特征.群落主要由亚热带科属组成,优势科为芸香科、五加科、茜草科、山茶科、壳斗科、鼠刺科、樟科等.优势树种也以相应的优势科属树种为主.群落中胸径≥3cm的个体密度较小,只有1964株/hm2;群落较为低矮,平均高度只有6m;乔木垂直分化不明显,树种水平分布不均.白云山的植被是次生植被和人工造林改造的结果,只在山谷少数植被没受人类干扰.其现状植被是天然次生植被和人工植被的混合体.在一些由马尾松发展起来的群落,天然侵入的阔叶树种已在主要层中居优势;群落中可见马尾松衰退的痕迹.     

Otolith development in larval and juvenile Schizothorax davidi: ontogeny and growth increment characteristics

Laboratory-reared Schizothorax davidi larvae and juveniles were examined to assess the formation and characteristics of David’s schizothoracin otoliths.Otolith development was observed and their formation period was verifi ed by monitoring larvae and juveniles of knownage.The results revealed that lapilli and sagittae developed before hatching,and the first otolith increment was identified at 2 days post hatching in both.The shape of lapilli was relatively stable during development compared with that of sagittae;however,growth of four sagittae and lapilli areas was consistent,but the posterior area grew faster than the anterior area and the ventral surface grew faster than the dorsal surface.Similarly,the sum length of the radius of the anterior and posterior areas on sagittae and lapilli were linearly and binomially related to total fish length,respectively.Moreover,daily deposition rates were validated by monitoring knownage larvae and juveniles.The increase in lapilli width was 1.88±0.080 0μm at the ninth increment,which reached a maximum and the decreased gradually toward the otolith edge,whereas that of sagittae increased more slowly.These results illustrate the developmental biology of S.davidi,which will aid in population conservation and fish stock management.     

Effects of varying densities on serum reproductive parameters in pen-reared juvenile female rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis> farms

The primary goal of this study was to assess the effect of varying densities on serum reproductive parameters of immature rainbow trout Oncorhynchus mykiss. Experimental trout were maintained in intensive, pen-reared farms for 300 days in fresh water reservoirs. Initial densities were 4.6, 6.6, and 8.6 kg/m³ (40, 60, 80 ind./m³), indicated as SD1, SD2, SD3, and final densities were 31.1, 40.6, 49.3 kg/m³, respectively. A summary of the ovarian stages were observed by histological examination. Serum E₂ (estradiol), T (testosterone) were evaluated by radioimmunoassay and FSH (follicle-stimulating-hormone), LH (luteinizing-hormone), vitellogenin, 17α,20β-P (17α,20βdihydroxy4-pregnen-3-one) were measured by enzyme-linked immunosorbent assay. Our findings demonstrated that ovarian development were retarded (from stage III to stage IV) at highest rearing density (SD3) after 180 days of intensive culture (over 40.6 kg/m³). In addition, we observed an inverse relationship between serum reproductive parameters and rearing density. Furthermore, compared to serum reproductive parameters of SD1, E₂, T, FSH, vitellogenin, 17α,20β-P, GSI and LH of two higher density groups decreased firstly and significantly at 60 (over 15.9 kg/m 3 ), 180 (over 31.7 kg/m 3 ), 180 (over 40.6 kg/m³), 240 (over 36 kg/m³), 240 (over 36 kg/m³), 240 (over 45 kg/m³) and 300 (over 49.3 kg/m³) days, respectively. Comparing serum reproductive parameters within the same ovarian development stage of rainbow trout from varying densities revealed that higher population density also led to significantly lower overall serum reproductive parameters. Overall, this study presents the reproductive, endocrinological parameters of juvenile female rainbow trout at high rearing densities and indicates the need for rainbow trout (114.44±5.21 g, 19.69±0.31 cm) that are initially stocked at 6.6 or 8.6 kg/m³ should be classified and subdivided into lower density after 180 days of farming (not over 31.7 kg/m³).     

一种基于自适应麦克风阵的智能天线实验平台

智能天线是第三代移动通信的关键技术之一,也是当前信号处理和通信领域的研究热点.在对智能天线算法进行理论分析和仿真的基础上,有必要构建一个灵活、通用的实验平台,用以验证各种智能天线算法的有效性和实用性.探讨了一种基于自适应麦克风阵的智能天线实验平台的实现方法,介绍了该系统的原理、结构和各部分的作用.     

Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades, K. alvarezii, Kappaphycus sp., and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.     

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Nitrogen and phosphorus intake by phytoplankton in the Xiamen Bay

This paper describes a time series experiment examining the nitrogen and phosphorus intake of natural phytoplankton communities by a microcosms approach.Seawater samples containing natural phytoplankton communities were collected from waters around Baozhu Islet in inner Xiamen Bay and around Qingyu Islet in the outer bay.The goal was to elucidate the relationship between phytoplankton population enhancement,the biological removal of nitrogen and phosphorus from the seawater,and the phytoplankton nitrogen an...     

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第二代小波在GPS双差观测值消噪中的应用

讨论第二代小波变换的基本原理和变换过程,并将第二代小波变换引入到GPS数据处理中.实验证明,第二代小波变换对GPS信号去除高频噪声具有一定的效果.