Importance of kelp-derived organic carbon to the scallop <Emphasis Type="Italic">Chlamys farreri</Emphasis> in an integrated multi-trophic aquaculture system

Bivalves and seaweeds are important cleaners that are widely used in integrated multi-trophic aquaculture (IMTA) systems. A beneficial relationship between seaweed and bivalve in the seaweed-based IMTA system has been confirmed, but the trophic importance of seaweed-derived particulate organic materials to the co-cultured bivalve is still unclear. We evaluated the trophic importance of the kelp Saccharina japonica to the co-cultured scallop Chlamys farreri in a typical IMTA farm in Sungo Bay (Weihai, North China). The dynamics of detritus carbon in the water were monitored during the culturing period. The proportion of kelp-derived organic matter in the diet of the co-cultured scallop was assessed via the stable carbon isotope method. Results showed that the detritus carbon in the water ranged from 75.52 to 265.19 μg/L, which was 25.6% to 73.8% of total particulate organic carbon (TPOC) during the study period. The amount of detritus carbon and its proportion in the TPOC changed throughout the culture cycle of the kelp. Stable carbon isotope analysis showed that the cultured scallop obtained 14.1% to 42.8% of its tissue carbon from the kelp, and that the percentages were closely correlated with the proportion of detritus carbon in the water (F =0.993, P= 0.003). Evaluation showed that for 17 000 tons (wet weight) of annual scallop production, the kelp contributed about 139.3 tons of carbon (535.8 tons of dry mass). This confirms that cultured kelp plays a similar trophic role in IMTA systems as it does in a natural kelp bed. It is a major contributor to the detritus pool and supplies a vital food source to filter-feeding scallops in the IMTA system, especially during winter and early spring when phytoplankton are scarce.     

Involvement of cyclic electron flow in irradiance stress responding and its potential regulation of the mechanisms in <Emphasis Type="Italic">Pyropia yezoensis</Emphasis>

Pyropia yezoensis, belongs to the genus of Porphyra before 2011, inhabit on intertidal zone rocks where irradiation changes dramatically, implying that the seaweed has gained certain mechanisms to survive a harsh environment. Based on the photosynthetic parameters with or without the inhibitors determined by a Dual-PAM-100 apparatus, we investigated the photosynthetic performance and the changes in electron flow that occurred during the algae were stressed with different light intensities previously. When the irradiation saturation was approaching, the CEF around PS I became crucial since the addition of inhibitors usually led to an increase in non-photochemical quenching. The inhibitor experiments showed that there were at least three different CEF pathways in Py. yezoensis and these pathways compensated each other. In addition to maintaining a proper ratio of ATP/NAD(P)H to support efficient photosynthesis, the potential roles of CEF might also include the regulation of different photoprotective mechanisms in Py. yezoensis. Under the regulation of CEF, chlororespiration is thought to transport electrons from the reduced plastoquinone (PQ) pool to oxygen in order to mitigate the reduction in the electron transfer chain. When irradiation was up to the high-grade stress conditions, the relative value of CEF began to decrease, which implied that the NADP⁺ pool or PQ⁺ pool was very small and that the electrons were transferred from reduced PS I to oxygen. The scavenging enzymes might be activated and the water-water cycle probably became an effective means of removing the active oxygen produced by the irradiation stressed Py. yezoensis. We believe that the different mechanisms could make up the photoprotective network to allow Py. yezoensis for survival in a highly variable light stress habitat, which may enlighten scientists in future studies on irradiance stress in other algae species.     

An integrative study of larval organogenesis of American shad <Emphasis Type="Italic">Alosa sapidissima</Emphasis> in histological aspects

We describe organogenesis at a histological level in American shad (Alosa sapidissima) larvae from 0 until 45 days after hatching (DAH). Larval development was divided into four stages based on the feeding mode, external morphological features, and structural changes in the organs: stage 1 (0–2 DAH), stage 2 (3–5 DAH), stage 3 (6–26 DAH) and stage 4 (27–45 DAH). At early stage 2 (3 DAH), American shad larvae developed the initial digestive and absorptive tissues, including the mouth and anal opening, buccopharyngeal cavity, oesophagus, incipient stomach, anterior and posterior intestine, differentiated hepatocytes, and exocrine pancreas. The digestive and absorptive capacity developed further in stages 2 to 3, at which time the pharyngeal teeth, taste buds, gut mucosa folds, differentiated stomach, and gastric glands could be observed. Four defined compartments were discernible in the heart at 4 DAH. From 3 to 13 DAH, the excretory systems started to develop, accompanied by urinary bladder opening, the appearance and development of primordial pronephros, and the proliferation and convolution of renal tubules. Primordial gills were detected at 2 DAH, the pseudobranch was visible at 6 DAH, and the filaments and lamellae proliferated rapidly during stage 3. The primordial swim bladder was first observed at 2 DAH and started to inflate at 9 DAH; from then on, it expanded constantly. The spleen was first observed at 8 DAH and the thymus was evident at 12 DAH. From stage 4 onwards, most organs essentially manifested an increase in size, number, and complexity of tissue structure.     

Constraints of molybdenite Re–Os and scheelite Sm–Nd ages on mineralization time of the Kukaazi Pb–Zn–Cu–W deposit,Western Kunlun,NW China

The Kukaazi Pb–Zn–Cu–W polymetallic deposit, located in the Western Kunlun orogenic belt, is a newly discovered skarn-type deposit. Ore bodies mainly occur in the forms of lenses and veins along beddings of the Mesoproterozoic metamorphic rocks. Three ore blocks, KI, KII, and KIII, have been outlined in different parts of the Kukaazi deposit in terms of mineral assemblages. The KI ore block is mainly composed of chalcopyrite, scheelite, pyrrhotite, sphalerite, galena and minor pyrite, arsenopyrite, and molybdenite, whereas the other two ore blocks are made up of galena, sphalerite, magnetite and minor arsenopyrite and pyrite. In this study, we obtained a molybdenite isochron Re–Os age of 450.5 ± 6.4 Ma (2σ, MSWD = 0.057) and a scheelite Sm–Nd isochron age of 426 ± 59 Ma (2σ, MSWD = 0.49) for the KI ore block. They are broadly comparable to the ages of granitoid in the region. Scheelite grains from the KI ore block contain high abundances of rare earth elements (REE, 42.0–95.7 ppm) and are enriched in light REE compared to heavy REE, with negative Eu anomalies (δEu = 0.13–0.55). They display similar REE patterns and Sm/Nd ratios to those of the coeval granitoids in the region. Moreover, they also have similar Sr and Nd isotopes [⁸⁷Sr/⁸⁶Sr = 0.7107–0.7118; ε_Nd(t) = ?4.1 to ?4.0] to those of such granitoids, implying that the tungsten-bearing fluids in the Kukaazi deposit probably originate from the granitic magmas. Our results first defined that the Early Paleozoic granitoids could lead to economic Mo–W–(Cu) mineralization at some favorable districts in the Western Kunlun orogenic belt and could be prospecting exploration targets.     

Geochemistry and geochronology of Late Jurassic and Early Cretaceous intrusions related to some Au (Sb) deposits in southern Anhui: a case study and review

Some Au deposits in southern Anhui Province have recently been found to be closely associated with Late Mesozoic intrusions. Typical examples include the Huashan Au (Sb) deposit and Au deposits at Zhaojialing, Wuxi, and Liaojia. In order to understand the mechanisms that led the formation of these Au deposits, we make detailed reviews on the geological characteristics of these Au deposits. Specifically, we present new LA-ICP-MS zircon U–Pb dating, along with elemental and Hf isotopic data from the Huashan Au (Sb) deposit. Our data suggests that the Huashan ore-related intrusions were emplaced during the Late Jurassic and Early Cretaceous periods (144–148 Ma). They are characterized by arc-magma features and high oxygen fugacity and are rich in inherited zircons. Zircon U–Pb ages and Lu–Hf isotopes from intrusions suggest that Proterozoic juvenile lithosphere is the main source of these intrusions. The regional geological history implies that lithosphere beneath southern Anhui was produced during a Proterozoic subduction and was fertilized with Au (Cu) in the process. Integrated with the results of previous studies, we inferred that Late Mesozoic intrusions formed by the remelting of the lithosphere could provide the metal endowment for the Au-rich deposits in southern Anhui.