Simulation of Hydrophysical Processes in Coastal Regions of the Sea

In the present work, we generalize the results of our investigations in the field of simulation of hydrophysical and ecological processes in coastal regions of various seas and some closed basins. The developed and applied mathematical models and the results of numerical experiments are briefly analyzed.     

Probing molecular-level transformations of dissolved organic matter: insights on photochemical degradation and protozoan modification of DOM from electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

Molecular-level characterization of natural organic matter (NOM) has been elusive due to the inherent complexity of natural organic mixtures and to the fact that individual components are often polar and macromolecular. Electrospray ionization (ESI) is a “soft” ionization technique that ionizes polar compounds from aqueous solution prior to injection into a mass spectrometer. The highest resolution and mass accuracy of compounds within NOM have been achieved when ESI is combined with an ultrahigh-resolution mass spectrometer such as the Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS). With this technique, individual molecules within a variety of natural organic mixtures can be detected and their elemental composition can be determined. At low mass-to-charge (m/z) ratio, the resolution is high enough to assign exact molecular formulas allowing specific components of these mixtures to be identified. In addition to molecular identification, we can now use ESI FT-ICR MS to examine molecular-level changes in different organic mixtures as a function of relevant geo-processes, such as microbial alterations and photochemistry. Here we present the results from the application of ESI FT-ICR MS to two geochemical questions: (1) the effect of photoirradiation on the molecular composition of fulvic acids and (2) the role of protozoan grazers in the modification of DOM in aquatic systems.     

Phototoxicity of pyrene and benzo[a]pyrene to embryo-larval stages of the Pacific oyster Crassostrea gigas

There is a growing body of evidence to suggest that certain polycyclic aromatic hydrocarbons (PAHs) pose a greater hazard to aquatic organisms than previously demonstrated, due to their potential to cause photo-induced toxicity when exposed to ultraviolet (UV) radiation. The consequences of photo-induced toxicity are reported here for embryo-larval stages of the pacific oyster Crassostrea gigas, following exposure to pyrene and benzo[a]pyrene. During laboratory investigations, significant increases in toxicity were observed in the presence of environmentally attainable levels of UV-radiation, compared with embryos exposed to PAH alone, at levels previously deemed to have little acute biological effect. The phototoxicity of pyrene and benzo[a]pyrene completely inhibited the development to the D-shell larval stage when embryos were simultaneously exposed to 5 microg l(-1) PAH and ultraviolet light (UVB = 6.3 +/- 0.1 microW/cm2 and UVA = 456.2 +/- 55 microW/cm2). A linear relationship was also demonstrated for benzo[a]pyrene phototoxicity with decreasing UV light intensity.     

Temperature fluctuations in the atmospheric surface layer over the thermally inhomogeneous underlying surface

The influence of both spatial and temporal temperature inhomogeneities of the underlying surface on the temperature field in an unstably stratified atmospheric surface layer is considered. The methods of correlation and spectral analyses are proposed to estimate statistical characteristics of surface-air temperature fluctuations caused by both turbulent mixing and inhomogeneities in the temperature of the underlying surface. Analysis of experimental data obtained from measurements in the atmospheric surface layer yields estimates for the contribution made by the time-dependent thermal properties of the underlying surface to the total variance of air-temperature fluctuations. It is shown that the additional air-temperature fluctuations generated by surface-temperature inhomogeneities and unrelated to shear flow may reach 70% and 30% of the total variance of measured fluctuations under variable cloudiness and clear skies, respectively. For the height z = 2 m within the wave-number range 2 × 10^?3 rad m^?1 < k < 0.1 rad m^?1, the contribution made by a spatial surface-temperature inhomogeneity to the variance of air-temperature fluctuations does not exceed 10% of the total variance. Correlation and spectral analyses of experimental data make it possible to isolate the spectra of properly turbulent fluctuations from the measured fluctuations and thus to obtain more accurate values of the universal function of similarity theory for temperature in the range of small wave numbers beyond the inertial range.     

Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a minimally-invasive biopsy approach

Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 1A (CYP1A), can be used to assess the impact of environmental contaminants on the health of free-ranging marine mammal populations. The objective of the present study was to measure CYP1A in skin and liver biopsies obtained from live harbour seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were captured from the Fraser River estuary, British Columbia, Canada, and temporarily held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) biopsies, obtained while seals were under general anaesthesia, yielded sufficient tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin O-deethylase activity. A short-term exposure experiment, in which harbour seals (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased hepatic and cutaneous CYP1A protein levels, consistent with observations in other mammals. This study is the first to measure CYP1A in skin and liver biopsies from live harbour seals and to report in vivo BNF-associated CYP1A induction in a marine mammal. The results demonstrate that microsamples collected using minimally-invasive techniques can provide toxicologically-relevant information form marine mammals.     

Design of a 3D Chirp Sub-bottom Imaging System

Chirp sub-bottom profilers are marine acoustic devices that use a known and repeatable source signature (1–24 kHz) to produce decimetre vertical resolution cross-sections of the sub-seabed. Here the design and development of the first true 3D Chirp system is described. When developing the design, critical factors that had to be considered included spatial aliasing, and precise positioning of sources and receivers. Full 3D numerical modelling of the combined source and receiver directivity was completed to determine optimal source and receiver geometries. The design incorporates four source transducers (1.5–13 kHz) that can be arranged into different configurations, including Maltese Cross, a square and two separated pairs. The receive array comprises 240 hydrophones in 60 groups whose group-centres are separated by 25 cm in both horizontal directions, with each hydrophone group containing four individual elements and a pre-amplifier. After careful consideration, it was concluded that the only way to determine with sufficient accuracy the source–receiver geometry, was to fix the sources and receivers within a rigid array. Positional information for the array is given by a Real Time Kinematic GPS and attitude system incorporating four antennas to give position, heading, pitch and roll. It is shown that this system offers vertical positioning accuracy with a root-mean-square (rms) error less than 2.6 cm, while the horizontal positioning rms error was less than 2.0 cm. The system is configured so that the Chirp source signature can be chosen by software aboard the acquisition vessel. The complete system is described and initial navigational and seismic data results are presented. These data demonstrate that the approach of using fixed source-receiver geometry combined with RTK navigation can provide complete 3D imaging of the sub-surface.     

Path following control system for a tanker ship model

A two-dimensional path following control system for autonomous marine surface vessels is presented. The guidance system is obtained through a way-point guidance scheme based on line-of-sight projection algorithm and the speed controller is achieved through state feedback linearization. A new approach concerning the calculation of a dynamic line-of-sight vector norm is presented which main idea is to improve the speed of the convergence of the vehicle to the desired path. The results obtained are compared with the traditional line-of-sight scheme. It is intended that the complete system will be tested and implemented in a model of the “Esso Osaka” tanker. The results of simulations are presented here showing the effectiveness of the system aiming in to be robust enough to perform tests either in tanks or lakes.     

Shoreline variability via empirical orthogonal function analysis: Part I temporal and spatial characteristics

Empirical orthogonal functions (EOFs) or principal components were used to extract the significant modes of shoreline variability from several data sets collected at three very different locations. Although EOFs have proven to be a valuable tool in the analysis of nearshore data, most applications have focused on the ability of the technique to describe cross-shore or profile variability. Here however, EOFs were used to help identify the dominant modes of longshore shoreline variability at Duck, North Carolina, the Gold Coast, Australia, and at several locations within the Columbia River Littoral Cell in the U.S. Pacific Northwest. In part one of this analysis, characteristic patterns of shoreline variability identified by the EOF analysis are described in detail. At each site, the dominant modes consisting of the first four eigenfunctions were found to describe nearly 95% of the total shoreline variability. At both Duck and the Gold Coast, several interesting longshore periodic features suggestive of sand waves were identified, while boundary effects related to natural headlands and navigational structures/entrances dominated the Pacific Northwest data sets.     

Time domain modelling of the transient asymmetric flooding of Ro-Ro ships

This work aims at contributing to improve knowledge on transient asymmetric flooding through theoretical and experimental research. First, a time domain theoretical model of ship motions and flooding is described. Results from experimental work are presented evidencing that transient asymmetric flooding may cause the capsizing of a Ro-Ro shaped barge. The theoretical model is used to predict the capsize of the Ro-Ro shaped barge. Reasonable agreement between experimental and theoretical results was found. Finally, a review of the European Gateway accident is given and the theoretical model is applied to the study of this type of accident. The conclusion is that this theoretical model, together with an accurate modelling of the flooding of machinery compartments, reproduces successfully the capsizing of the European Gateway due to transient asymmetric flooding. Therefore, the internal arrangement of Ro-Ro ships should be carefully studied at the design stage in order to avoid this phenomenon.